{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670319751691136.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1152/ajprenal.00341.2003"}},{"identifier":{"@type":"URI","@value":"https://www.physiology.org/doi/pdf/10.1152/ajprenal.00341.2003"}}],"dc:title":[{"@value":"Distribution of the tight junction proteins ZO-1, occludin, and claudin-4, -8, and -12 in bladder epithelium"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>In mammals, the bladder stores urine without permitting the passage of urine contents into the bloodstream, a function, in part, of the uroepithelial-associated tight junction complex. The protein constituents that make up this high-resistance barrier in the bladder are currently unknown, although the claudins, a multigene family, are thought to govern paracellular transport in other epithelia. Reverse transcriptase-polymerase chain reaction analysis was used to define that mRNA for claudin-2, -4, -8, -12, and -13 was expressed in mouse bladder tissue. The localization of these claudins, as well as the tight junction-associated proteins zonula occludens-1 (ZO-1) and occludin, within the bladder epithelium was determined by immunofluorescence microscopy. As expected, occludin and ZO-1 were localized to the tight junctions of rat, mouse, and rabbit umbrella cells. Intriguingly, ZO-1 in mouse epithelium, ZO-1 in the dome region of rabbit bladders and occludin in rat and mouse bladders were also expressed in the underlying intermediate and basal cell layers. Claudin-4, -8, and -12 were found in the umbrella cell tight junction; however, additional staining of claudin-4 was observed along the sites of cell-cell contact in the underlying cell layers of rat, mouse, and rabbit tissue. No claudin-2 staining was associated with tight junctions in the uroepithelium. Our results indicate that claudin-4, -8, and -12 are expressed in umbrella cells, where they may impart the high-resistance phenotype associated with this cell type, and that in some instances tight junction proteins are also associated at the sites of cell contact of the underlying cell layers, perhaps playing some role in cell-cell adhesion.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670319751691140","@type":"Researcher","foaf:name":[{"@value":"Prasad Acharya"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319751691141","@type":"Researcher","foaf:name":[{"@value":"Jonathan Beckel"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319751691138","@type":"Researcher","foaf:name":[{"@value":"Wily G. Ruiz"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319751691136","@type":"Researcher","foaf:name":[{"@value":"Edward Wang"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319751691142","@type":"Researcher","foaf:name":[{"@value":"Raul Rojas"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319751691137","@type":"Researcher","foaf:name":[{"@value":"Lori Birder"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319751691139","@type":"Researcher","foaf:name":[{"@value":"Gerard Apodaca"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"1931857X"},{"@type":"EISSN","@value":"15221466"}],"prism:publicationName":[{"@value":"American Journal of Physiology-Renal Physiology"}],"dc:publisher":[{"@value":"American Physiological Society"}],"prism:publicationDate":"2004-08","prism:volume":"287","prism:number":"2","prism:startingPage":"F305","prism:endingPage":"F318"},"reviewed":"false","url":[{"@id":"https://www.physiology.org/doi/pdf/10.1152/ajprenal.00341.2003"}],"createdAt":"2004-04-13","modifiedAt":"2021-06-15","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050565162619004416","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Claudin 4 in pancreatic β cells is involved in regulating the functional state of adult islets"}]},{"@id":"https://cir.nii.ac.jp/crid/1050869456402742272","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Collagen 17A1 in the Urothelium Regulates Epithelial Cell Integrity and Local Immunologic Responses in Obstructive Uropathy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004234785308032","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Loss of β1‐integrin from urothelium results in overactive bladder and incontinence in mice: a mechanosensory rather than structural phenotype"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565169386607744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The Reversible Increase in Tight Junction Permeability Induced by Capsaicin Is Mediated via Cofilin-Actin Cytoskeletal Dynamics and Decreased Level of Occludin"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567180191540224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Aquaporin‐2 plays an important role in water transportation through the bladder wall in rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567187466363776","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Claudin-4 Deficiency Results in Urothelial Hyperplasia and Lethal Hydronephrosis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848660684647552","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Sensor Mechanism and Afferent Signal Transduction of the Urinary Bladder: Special Focus on transient receptor potential Ion Channels"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681401038592","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Restricted Localization of Claudin-16 at the Tight Junction in the Thick Ascending Limb of Henle's Loop Together with Claudins 3, 4, and 10 in Bovine Nephrons"},{"@language":"ja","@value":"牛クローディン-16の腎尿細管ヘンレの太い上行脚タイトジャンクションにおける局在,ならびにクローディン-3,-4,-10との共在"},{"@value":"Restricted localization of claudin-16 at the tight junction in the thick ascending limb of Henle's loop together with claudin 3, 4, and 10 in bovine nephrons"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1152/ajprenal.00341.2003"},{"@type":"CROSSREF","@value":"10.1096/fj.12-223404_references_DOI_VO4QwxYldPQayhZAN6skFHvssZW"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0079954_references_DOI_VO4QwxYldPQayhZAN6skFHvssZW"},{"@type":"CROSSREF","@value":"10.1002/nau.23715_references_DOI_VO4QwxYldPQayhZAN6skFHvssZW"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0052272_references_DOI_VO4QwxYldPQayhZAN6skFHvssZW"},{"@type":"CROSSREF","@value":"10.1016/j.ajpath.2024.04.009_references_DOI_VO4QwxYldPQayhZAN6skFHvssZW"},{"@type":"CROSSREF","@value":"10.1111/j.1757-5672.2010.00074.x_references_DOI_VO4QwxYldPQayhZAN6skFHvssZW"},{"@type":"CROSSREF","@value":"10.1292/jvms.68.453_references_DOI_VO4QwxYldPQayhZAN6skFHvssZW"},{"@type":"CROSSREF","@value":"10.1002/2211-5463.12735_references_DOI_VO4QwxYldPQayhZAN6skFHvssZW"}]}