{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670319774987776.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1530/rep.1.00735"}},{"identifier":{"@type":"URI","@value":"https://rep.bioscientifica.com/view/journals/rep/130/4/1300485.xml"}},{"identifier":{"@type":"URI","@value":"https://academic.oup.com/reproduction/article-pdf/130/4/485/64607369/1300485.pdf"}}],"dc:title":[{"@value":"Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes\n                    <i>in vitro</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n                  <jats:p>In this study concentration and composition of non-esterified fatty acids (NEFA) in follicular fluid (FF) of high-yielding dairy cows were determined during the period of negative energy balance (NEB) early post partum. NEFA were then added during in vitro maturation at concentrations measured previously in FF to evaluate their effect on the oocyte’s developmental competence. At 16 and 44 days post partum, FF of the dominant follicle and blood were collected from nine high-yielding dairy cows. Samples were analysed for NEFA concentration and composition. NEFA concentrations in FF (0.2–0.6 mmol/l) during NEB remained ± 40% lower compared with serum (0.4–1.2 mmol/l). The NEFA composition differed significantly between serum and FF with oleic acid (OA), palmitic acid (PA) and stearic acid (SA) being the predominant fatty acids in FF. Based on these results, 5115 oocytes were matured for 24 h in serum-free media with or without (negative control) the addition of 0.200 mmol/l OA, 0.133 mmol/l PA or 0.067 mmol/l SA dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in SOF medium. Addition of PA or SA during oocyte maturation had negative effects on maturation, fertilization and cleavage rate and blastocyst yield. More (late) apoptotic cumulus cells were observed in cumulus–oocyte complexes matured in the presence of SA or PA. Ethanol or OA had no effect. These in vitro results suggest that NEB may hamper fertility of high-yielding dairy cows through increased NEFA concentrations in FF affecting oocyte quality.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670319774987781","@type":"Researcher","foaf:name":[{"@value":"J L M R Leroy"}],"jpcoar:affiliationName":[{"@value":"Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319774987649","@type":"Researcher","foaf:name":[{"@value":"T Vanholder"}],"jpcoar:affiliationName":[{"@value":"Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319774987648","@type":"Researcher","foaf:name":[{"@value":"B Mateusen"}],"jpcoar:affiliationName":[{"@value":"Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319774987780","@type":"Researcher","foaf:name":[{"@value":"A Christophe"}],"jpcoar:affiliationName":[{"@value":"Department of Internal Medicine, Division of Nutrition, Faculty of Medicine, Ghent University, De Pintelaan 185, 9000 Ghent, Belgium"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319774987777","@type":"Researcher","foaf:name":[{"@value":"G Opsomer"}],"jpcoar:affiliationName":[{"@value":"Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319774987778","@type":"Researcher","foaf:name":[{"@value":"A de Kruif"}],"jpcoar:affiliationName":[{"@value":"Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319774987776","@type":"Researcher","foaf:name":[{"@value":"G Genicot"}],"jpcoar:affiliationName":[{"@value":"Institut des Sciences de la Vie, Unité des Sciences Vétérinaires, Catholic University of Louvain, Place Croix du Sud 5 box 10, B-1348 Louvain-la-Neuve, Belgium"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670319774987779","@type":"Researcher","foaf:name":[{"@value":"A Van Soom"}],"jpcoar:affiliationName":[{"@value":"Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"14701626"},{"@type":"EISSN","@value":"17417899"}],"prism:publicationName":[{"@value":"Reproduction"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2005-10-01","prism:volume":"130","prism:number":"4","prism:startingPage":"485","prism:endingPage":"495"},"reviewed":"false","dc:rights":["https://academic.oup.com/pages/standard-publication-reuse-rights"],"url":[{"@id":"https://rep.bioscientifica.com/view/journals/rep/130/4/1300485.xml"},{"@id":"https://academic.oup.com/reproduction/article-pdf/130/4/485/64607369/1300485.pdf"}],"createdAt":"2005-09-23","modifiedAt":"2026-01-07","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050587981427282944","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Postpartum cows showed high oocyte triacylglycerols concurrently with high plasma free fatty acids"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567184497158272","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Palmitic acid induces ceramide accumulation, mitochondrial protein hyperacetylation, and mitochondrial dysfunction in porcine oocytes†"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567185421534592","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Palmitic acid stimulates interleukin‐8 via the TLR4/NF‐κB/ROS pathway and induces mitochondrial dysfunction in bovine oviduct epithelial cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567185438698496","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Midkine and cytoplasmic maturation of mammalian oocytes in the context of ovarian follicle physiology"}]},{"@id":"https://cir.nii.ac.jp/crid/1360588380618348160","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Impact of negative energy balance and postpartum diseases during the transition period on oocyte quality and embryonic development in dairy cows"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206337116288","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Factors Influencing the Biochemical Markers for Predicting Mammalian Oocyte Quality"}]},{"@id":"https://cir.nii.ac.jp/crid/1390014183333639808","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Effects of short-term dietary supplementation on the number of ovarian follicles, quantity and quality of oocytes, and <i>in vitro</i> embryo production in Japanese Black cows"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681313734272","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Multifactorial Analysis of the Follicular Environment is Predictive of Oocyte          Morphology in Cattle"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282681406123904","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Improvement of Superovulatory Response and Pregnancy Rate after Transfer of          Embryos Recovered from Japanese Black Cows Fed Rumen Bypass Polyunsaturated Fatty          Acids"}]},{"@id":"https://cir.nii.ac.jp/crid/1390300379549915264","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Lipopolysaccharide-binding protein in follicular fluid is associated with the follicular inflammatory status and granulosa cell steroidogenesis in dairy cows"}]},{"@id":"https://cir.nii.ac.jp/crid/1390306200188066560","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The effect 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