{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320127632128.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1182/blood-2007-07-103309"}},{"identifier":{"@type":"URI","@value":"http://ashpublications.org/blood/article-pdf/111/7/3665/1481133/zh800708003665.pdf"}}],"dc:title":[{"@value":"VAMP-8 segregates mast cell–preformed mediator exocytosis from cytokine trafficking pathways"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>Inflammatory responses by mast cells are characterized by massive exocytosis of prestored granular mediators followed by cytokine/chemokine release. The vesicular trafficking mechanisms involved remain poorly understood. Vesicular-associated membrane protein-8 (VAMP-8), a member of the soluble N-ethylmaleimide–sensitive factor (NSF) attachment protein receptor (SNARE) family of fusion proteins initially characterized in endosomal and endosomal-lysosomal fusion, may also function in regulated exocytosis. Here we show that in bone marrow–derived mast cells (BMMCs) VAMP-8 partially colocalized with secretory granules and redistributed upon stimulation. This was associated with increased SNARE complex formation with the target t-SNAREs, SNAP-23 and syntaxin-4. VAMP-8–deficient BMMCs exhibited a markedly reduced degranulation response after IgE+ antigen-, thapsigargin-, or ionomycin-induced stimulation. VAMP-8–deficient mice also showed reduced plasma histamine levels in passive systemic anaphylaxis experiments, while cytokine/chemokine release was not affected. Unprocessed TNF accumulated at the plasma membrane where it colocalized with a VAMP-3–positive vesicular compartment but not with VAMP-8. The findings demonstrate that VAMP-8 segregates secretory lysosomal granule exocytosis in mast cells from cytokine/chemokine molecular trafficking pathways.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320127632261","@type":"Researcher","foaf:name":[{"@value":"Neeraj Tiwari"}],"jpcoar:affiliationName":[{"@value":"Inserm U699, Paris, France;"},{"@value":"Université Paris 7-Denis Diderot, Faculté de Médecine, Site Xavier Bichat, Paris, France;"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320127632262","@type":"Researcher","foaf:name":[{"@value":"Cheng-Chun Wang"}],"jpcoar:affiliationName":[{"@value":"Membrane Biology Laboratory, Institute of Molecular and Cellular Biology, Proteos, Singapore;"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320127632257","@type":"Researcher","foaf:name":[{"@value":"Cristiana Brochetta"}],"jpcoar:affiliationName":[{"@value":"Inserm U699, Paris, France;"},{"@value":"Université Paris 7-Denis Diderot, Faculté de Médecine, Site Xavier Bichat, Paris, France;"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320127632264","@type":"Researcher","foaf:name":[{"@value":"Gou Ke"}],"jpcoar:affiliationName":[{"@value":"Membrane Biology Laboratory, Institute of Molecular and Cellular Biology, Proteos, Singapore;"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320127632256","@type":"Researcher","foaf:name":[{"@value":"Francesca Vita"}],"jpcoar:affiliationName":[{"@value":"Department of Physiology and Pathology, University of Trieste, Trieste, Italy; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320127632258","@type":"Researcher","foaf:name":[{"@value":"Zeng Qi"}],"jpcoar:affiliationName":[{"@value":"Membrane Biology Laboratory, Institute of Molecular and Cellular Biology, Proteos, Singapore;"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320127632260","@type":"Researcher","foaf:name":[{"@value":"Juan Rivera"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Immune Cell Signaling, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320127632263","@type":"Researcher","foaf:name":[{"@value":"Maria Rosa Soranzo"}],"jpcoar:affiliationName":[{"@value":"Department of Physiology and Pathology, University of Trieste, Trieste, Italy; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320127632259","@type":"Researcher","foaf:name":[{"@value":"Giuliano Zabucchi"}],"jpcoar:affiliationName":[{"@value":"Department of Physiology and Pathology, University of Trieste, Trieste, Italy; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320127632266","@type":"Researcher","foaf:name":[{"@value":"Wanjin Hong"}],"jpcoar:affiliationName":[{"@value":"Membrane Biology Laboratory, Institute of Molecular and Cellular Biology, Proteos, Singapore;"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320127632265","@type":"Researcher","foaf:name":[{"@value":"Ulrich Blank"}],"jpcoar:affiliationName":[{"@value":"Inserm U699, Paris, France;"},{"@value":"Université Paris 7-Denis Diderot, Faculté de Médecine, Site Xavier Bichat, Paris, France;"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00064971"},{"@type":"EISSN","@value":"15280020"}],"prism:publicationName":[{"@value":"Blood"}],"dc:publisher":[{"@value":"American Society of Hematology"}],"prism:publicationDate":"2008-04-01","prism:volume":"111","prism:number":"7","prism:startingPage":"3665","prism:endingPage":"3674"},"reviewed":"false","url":[{"@id":"http://ashpublications.org/blood/article-pdf/111/7/3665/1481133/zh800708003665.pdf"}],"createdAt":"2008-01-19","modifiedAt":"2021-09-02","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050283687764469760","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Vesicular Polyamine Transporter Mediates Vesicular Storage and Release of Polyamine from Mast Cells"},{"@value":"Vesicular polyamine transporter mediates vesicular storage and release of polyamines from mast cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283690771443456","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Omalizumab inhibits acceleration of FcεRI-mediated responsiveness of immature human mast cells by immunoglobulin E"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283690957097088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Crucial role for autophagy in degranulation of mast cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706983353088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The suppression of IgE-mediated histamine release from mast cells following exocytic exclusion of biodegradable polymeric nanoparticles"}]},{"@id":"https://cir.nii.ac.jp/crid/1360290617731089152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Rab44 isoforms similarly promote lysosomal exocytosis, but exhibit differential localization in mast cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567179933591040","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Phosphorylation of syntaxin‐3 at Thr 14 negatively regulates exocytosis in RBL‐2H3 mast cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567181955116544","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Real-time imaging of mast cell degranulation in vitro and in vivo"}]},{"@id":"https://cir.nii.ac.jp/crid/1360584340525580288","@type":"Article","resourceType":"学術雑誌論文(journal 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