{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320156573824.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1007/s00253-008-1393-6"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1007/s00253-008-1393-6.pdf"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/article/10.1007/s00253-008-1393-6/fulltext.html"}},{"identifier":{"@type":"URI","@value":"http://link.springer.com/content/pdf/10.1007/s00253-008-1393-6"}},{"identifier":{"@type":"PMID","@value":"18340445"}}],"dc:title":[{"@value":"Isoflavone aglycones production from isoflavone glycosides by display of β-glucosidase from Aspergillus oryzae on yeast cell surface"}],"description":[{"notation":[{"@value":"For efficient production of isoflavone aglycones from soybean isoflavones, we isolated three novel types of beta-glucosidase (BGL1, BGL3, and BGL5) from the filamentous fungi Aspergillus oryzae. Three enzymes were independently displayed on the cell surface of a yeast Saccharomyces cerevisiae as a fusion protein with alpha-agglutinin. Three beta-glucosidase-displaying yeast strains hydrolyzed isoflavone glycosides efficiently but exhibited different substrate specificities. Among these beta-glucosidases, BGL1 exhibited the highest activity and also broad substrate specificity to isoflavone glycosides. Although glucose released from isoflavone glycosides are generally known to inhibit beta-glucosidase, the residual ratio of isoflavone glycosides in the reaction mixture with BGL1-displaying yeast strain (Sc-BGL1) reached approximately 6.2%, and the glucose concentration in the reaction mixture was maintained at lower level. This result indicated that Sc-BGL1 assimilated the glucose before they inhibited the hydrolysis reaction, and efficient production of isoflavone aglycones was achieved by engineered yeast cells displaying beta-glucosidase."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320156573828","@type":"Researcher","foaf:name":[{"@value":"Masahiko Kaya"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320156573696","@type":"Researcher","foaf:name":[{"@value":"Junji Ito"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320156573830","@type":"Researcher","foaf:name":[{"@value":"Atsushi Kotaka"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320156573826","@type":"Researcher","foaf:name":[{"@value":"Kengo Matsumura"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320156573824","@type":"Researcher","foaf:name":[{"@value":"Hiroki Bando"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320156573833","@type":"Researcher","foaf:name":[{"@value":"Hiroshi Sahara"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320156573697","@type":"Researcher","foaf:name":[{"@value":"Chiaki Ogino"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320156573825","@type":"Researcher","foaf:name":[{"@value":"Seiji Shibasaki"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320156573831","@type":"Researcher","foaf:name":[{"@value":"Kouichi Kuroda"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320156573829","@type":"Researcher","foaf:name":[{"@value":"Mitsuyoshi Ueda"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320156573827","@type":"Researcher","foaf:name":[{"@value":"Akihiko Kondo"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320156573832","@type":"Researcher","foaf:name":[{"@value":"Yoji Hata"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"01757598"},{"@type":"EISSN","@value":"14320614"}],"prism:publicationName":[{"@value":"Applied Microbiology and Biotechnology"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2008-05","prism:volume":"79","prism:number":"1","prism:startingPage":"51","prism:endingPage":"60"},"reviewed":"false","dc:rights":["http://www.springer.com/tdm"],"url":[{"@id":"http://link.springer.com/content/pdf/10.1007/s00253-008-1393-6.pdf"},{"@id":"http://link.springer.com/article/10.1007/s00253-008-1393-6/fulltext.html"},{"@id":"http://link.springer.com/content/pdf/10.1007/s00253-008-1393-6"}],"createdAt":"2008-03-13","modifiedAt":"2020-02-06","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Aspergillus%20oryzae","dc:title":"Aspergillus oryzae"},{"@id":"https://cir.nii.ac.jp/all?q=Hydrolysis","dc:title":"Hydrolysis"},{"@id":"https://cir.nii.ac.jp/all?q=Recombinant%20Fusion%20Proteins","dc:title":"Recombinant Fusion Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Genetic%20Vectors","dc:title":"Genetic Vectors"},{"@id":"https://cir.nii.ac.jp/all?q=Saccharomyces%20cerevisiae","dc:title":"Saccharomyces cerevisiae"},{"@id":"https://cir.nii.ac.jp/all?q=Isoflavones","dc:title":"Isoflavones"},{"@id":"https://cir.nii.ac.jp/all?q=Substrate%20Specificity","dc:title":"Substrate Specificity"},{"@id":"https://cir.nii.ac.jp/all?q=Glucose","dc:title":"Glucose"},{"@id":"https://cir.nii.ac.jp/all?q=Transformation,%20Genetic","dc:title":"Transformation, Genetic"},{"@id":"https://cir.nii.ac.jp/all?q=Cellulases","dc:title":"Cellulases"},{"@id":"https://cir.nii.ac.jp/all?q=Glycosides","dc:title":"Glycosides"},{"@id":"https://cir.nii.ac.jp/all?q=Cloning,%20Molecular","dc:title":"Cloning, Molecular"},{"@id":"https://cir.nii.ac.jp/all?q=Plasmids","dc:title":"Plasmids"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360025023080839808","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Aspergillus oryzae strains heterogeneously produce plant polysaccharide degradation-related 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