{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320233139712.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1101/gad.257048.114"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1101/gad.257048.114"}}],"dc:title":[{"@value":"Protein arginine methyltransferase CARM1 attenuates the paraspeckle-mediated nuclear retention of mRNAs containing IR<i>Alu</i>s"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>In many cells, mRNAs containing inverted repeated <jats:italic>Alu</jats:italic> elements (IR<jats:italic>Alu</jats:italic>s) in their 3′ untranslated regions (UTRs) are inefficiently exported to the cytoplasm. Such nuclear retention correlates with paraspeckle-associated protein complexes containing p54<jats:sup>nrb</jats:sup>. However, nuclear retention of mRNAs containing IR<jats:italic>Alu</jats:italic>s is variable, and how regulation of retention and export is achieved is poorly understood. Here we show one mechanism of such regulation via the arginine methyltransferase CARM1 (coactivator-associated arginine methyltransferase 1). We demonstrate that disruption of CARM1 enhances the nuclear retention of mRNAs containing IR<jats:italic>Alu</jats:italic>s. CARM1 regulates this nuclear retention pathway at two levels: CARM1 methylates the coiled-coil domain of p54<jats:sup>nrb</jats:sup>, resulting in reduced binding of p54<jats:sup>nrb</jats:sup> to mRNAs containing IR<jats:italic>Alu</jats:italic>s, and also acts as a transcription regulator to suppress <jats:italic>NEAT1</jats:italic> transcription, leading to reduced paraspeckle formation. These actions of CARM1 work together synergistically to regulate the export of transcripts containing IR<jats:italic>Alu</jats:italic>s from paraspeckles under certain cellular stresses, such as poly(I:C) treatment. This work demonstrates how a post-translational modification of an RNA-binding protein affects protein–RNA interaction and also uncovers a mechanism of transcriptional regulation of the long noncoding RNA <jats:italic>NEAT1</jats:italic>.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320233139842","@type":"Researcher","foaf:name":[{"@value":"Shi-Bin Hu"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320233139840","@type":"Researcher","foaf:name":[{"@value":"Jian-Feng Xiang"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320233139841","@type":"Researcher","foaf:name":[{"@value":"Xiang Li"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320233139712","@type":"Researcher","foaf:name":[{"@value":"Yefen Xu"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320233139716","@type":"Researcher","foaf:name":[{"@value":"Wei Xue"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320233139717","@type":"Researcher","foaf:name":[{"@value":"Min Huang"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320233139718","@type":"Researcher","foaf:name":[{"@value":"Catharine C. Wong"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320233139714","@type":"Researcher","foaf:name":[{"@value":"Cari A. Sagum"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320233139713","@type":"Researcher","foaf:name":[{"@value":"Mark T. Bedford"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320233139719","@type":"Researcher","foaf:name":[{"@value":"Li Yang"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320233139968","@type":"Researcher","foaf:name":[{"@value":"Donghang Cheng"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320233139715","@type":"Researcher","foaf:name":[{"@value":"Ling-Ling Chen"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"08909369"},{"@type":"EISSN","@value":"15495477"}],"prism:publicationName":[{"@value":"Genes & Development"}],"dc:publisher":[{"@value":"Cold Spring Harbor Laboratory"}],"prism:publicationDate":"2015-03-15","prism:volume":"29","prism:number":"6","prism:startingPage":"630","prism:endingPage":"645"},"reviewed":"false","url":[{"@id":"https://syndication.highwire.org/content/doi/10.1101/gad.257048.114"}],"createdAt":"2015-03-19","modifiedAt":"2021-11-17","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004233537805184","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Paraspeckles modulate the intranuclear distribution of paraspeckle-associated Ctn RNA"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004234918185088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Molecular dissection of nuclear paraspeckles: towards understanding the emerging world of the RNP milieu"}]},{"@id":"https://cir.nii.ac.jp/crid/1360013168729832832","@type":"Article","resourceType":"学術雑誌論文(journal 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