{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320271265152.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1097/sla.0b013e31820563a8"}},{"identifier":{"@type":"URI","@value":"http://journals.lww.com/00000658-201102000-00032"}},{"identifier":{"@type":"PMID","@value":"21217515"}}],"dc:title":[{"@value":"Angiogenesis in Wounds Treated by Microdeformational Wound Therapy"}],"description":[{"notation":[{"@value":"Mechanical forces play an important role in tissue neovascularization and are a constituent part of modern wound therapies. The mechanisms by which vacuum assisted closure (VAC) modulates wound angiogenesis are still largely unknown.To investigate how VAC treatment affects wound hypoxia and related profiles of angiogenic factors as well as to identify the anatomical characteristics of the resultant, newly formed vessels.Wound neovascularization was evaluated by morphometric analysis of CD31-stained wound cross-sections as well as by corrosion casting analysis. Wound hypoxia and mRNA expression of HIF-1α and associated angiogenic factors were evaluated by pimonidazole hydrochloride staining and quantitative reverse transcription-polymerase chain reaction (RT-PCR), respectively. Vascular endothelial growth factor (VEGF) protein levels were determined by western blot analysis.VAC-treated wounds were characterized by the formation of elongated vessels aligned in parallel and consistent with physiological function, compared to occlusive dressing control wounds that showed formation of tortuous, disoriented vessels. Moreover, VAC-treated wounds displayed a well-oxygenated wound bed, with hypoxia limited to the direct proximity of the VAC-foam interface, where higher VEGF levels were found. By contrast, occlusive dressing control wounds showed generalized hypoxia, with associated accumulation of HIF-1α and related angiogenic factors.The combination of established gradients of hypoxia and VEGF expression along with mechanical forces exerted by VAC therapy was associated with the formation of more physiological blood vessels compared to occlusive dressing control wounds. These morphological changes are likely a necessary condition for better wound healing."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320271265163","@type":"Researcher","foaf:name":[{"@value":"Paolo Erba"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320271265158","@type":"Researcher","foaf:name":[{"@value":"Rei Ogawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320271265162","@type":"Researcher","foaf:name":[{"@value":"Maximilian Ackermann"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320271265152","@type":"Researcher","foaf:name":[{"@value":"Avner Adini"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320271265155","@type":"Researcher","foaf:name":[{"@value":"Lino F. Miele"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320271265160","@type":"Researcher","foaf:name":[{"@value":"Pouya Dastouri"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320271265159","@type":"Researcher","foaf:name":[{"@value":"Doug Helm"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320271265157","@type":"Researcher","foaf:name":[{"@value":"Steven J. Mentzer"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320271265154","@type":"Researcher","foaf:name":[{"@value":"Robert J. D’Amato"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320271265153","@type":"Researcher","foaf:name":[{"@value":"George F. Murphy"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320271265161","@type":"Researcher","foaf:name":[{"@value":"Moritz A. Konerding"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320271265156","@type":"Researcher","foaf:name":[{"@value":"Dennis P. Orgill"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00034932"}],"prism:publicationName":[{"@value":"Annals of Surgery"}],"dc:publisher":[{"@value":"Ovid Technologies (Wolters Kluwer Health)"}],"prism:publicationDate":"2011-02","prism:volume":"253","prism:number":"2","prism:startingPage":"402","prism:endingPage":"409"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://journals.lww.com/00000658-201102000-00032"}],"createdAt":"2011-01-07","modifiedAt":"2021-04-10","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Vascular%20Endothelial%20Growth%20Factor%20A","dc:title":"Vascular Endothelial Growth Factor A"},{"@id":"https://cir.nii.ac.jp/all?q=Wound%20Healing","dc:title":"Wound Healing"},{"@id":"https://cir.nii.ac.jp/all?q=Reverse%20Transcriptase%20Polymerase%20Chain%20Reaction","dc:title":"Reverse Transcriptase Polymerase Chain Reaction"},{"@id":"https://cir.nii.ac.jp/all?q=Neovascularization,%20Physiologic","dc:title":"Neovascularization, Physiologic"},{"@id":"https://cir.nii.ac.jp/all?q=Occlusive%20Dressings","dc:title":"Occlusive Dressings"},{"@id":"https://cir.nii.ac.jp/all?q=Biomechanical%20Phenomena","dc:title":"Biomechanical Phenomena"},{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Inbred%20C57BL","dc:title":"Mice, Inbred C57BL"},{"@id":"https://cir.nii.ac.jp/all?q=Oxygen","dc:title":"Oxygen"},{"@id":"https://cir.nii.ac.jp/all?q=Platelet%20Endothelial%20Cell%20Adhesion%20Molecule-1","dc:title":"Platelet Endothelial Cell Adhesion Molecule-1"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Microvessels","dc:title":"Microvessels"},{"@id":"https://cir.nii.ac.jp/all?q=Granulation%20Tissue","dc:title":"Granulation Tissue"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Negative-Pressure%20Wound%20Therapy","dc:title":"Negative-Pressure Wound Therapy"},{"@id":"https://cir.nii.ac.jp/all?q=Skin","dc:title":"Skin"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285706295161088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Noninvasive induction of angiogenesis in tissues by external suction: sequential optimization for use in reconstructive surgery"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567184861326976","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Moderate-Intensity Intermittent External Volume Expansion Optimizes the Soft-Tissue Response in a Murine Model"}]},{"@id":"https://cir.nii.ac.jp/crid/1360580232172453504","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Novel cell culture system for monitoring cells during continuous and variable negative‐pressure wound therapy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360584340696482816","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Negative-Pressure Wound Therapy: What We Know and What We Need to Know"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848659838164864","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Noninvasive Flap Preconditioning by Foam-Mediated External Suction Improves the Survival of Fasciocutaneous Axial-Pattern Flaps in a Type 2 Diabetic Murine Model"}]},{"@id":"https://cir.nii.ac.jp/crid/1360861707158061568","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Accelerated Angiogenesis of Human Umbilical Vein Endothelial Cells Under Negative Pressure Was Associated With the Regulation of Gene Expression Involved in the Proliferation and Migration"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680250633344","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Use of Negative Pressure Wound Therapy for Lower Limb Bypass Incisions"}]},{"@id":"https://cir.nii.ac.jp/crid/1390845713057528192","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"ja","@value":"後腹膜膿瘍の胸腔内穿破に起因する膿胸に対して，開窓術後に局所陰圧閉鎖療法により残存腔を閉鎖し得た1例"},{"@language":"en","@value":"A case of empyema due to rupture of retroperitoneal abscess successfully treated with negative pressure wound therapy after open-window thoracostomy"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1097/sla.0b013e31820563a8"},{"@type":"OPENAIRE","@value":"doi_dedup___::c2737391112417b45e381e0b9437c328"},{"@type":"CROSSREF","@value":"10.1007/s10456-017-9586-1_references_DOI_2SFLtAIbVqq3pt70Bqabe6BHMxq"},{"@type":"CROSSREF","@value":"10.1097/prs.0000000000005038_references_DOI_2SFLtAIbVqq3pt70Bqabe6BHMxq"},{"@type":"CROSSREF","@value":"10.1111/srt.13262_references_DOI_2SFLtAIbVqq3pt70Bqabe6BHMxq"},{"@type":"CROSSREF","@value":"10.1007/5584_2023_773_references_DOI_2SFLtAIbVqq3pt70Bqabe6BHMxq"},{"@type":"CROSSREF","@value":"10.3400/avd.oa.17-00052_references_DOI_2SFLtAIbVqq3pt70Bqabe6BHMxq"},{"@type":"CROSSREF","@value":"10.2995/jacsurg.33.145_references_DOI_2SFLtAIbVqq3pt70Bqabe6BHMxq"},{"@type":"CROSSREF","@value":"10.1097/sap.0000000000003298_references_DOI_2SFLtAIbVqq3pt70Bqabe6BHMxq"},{"@type":"CROSSREF","@value":"10.1097/prs.0000000000003190_references_DOI_2SFLtAIbVqq3pt70Bqabe6BHMxq"}]}