{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320338076160.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1261/rna.029769.111"}},{"identifier":{"@type":"URI","@value":"https://syndication.highwire.org/content/doi/10.1261/rna.029769.111"}}],"dc:title":[{"@value":"Production of artificial piRNAs in flies and mice"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>In animals a discrete class of small RNAs, the piwi-interacting RNAs (piRNAs), guard germ cell genomes against the activity of mobile genetic elements. piRNAs are generated, via an unknown mechanism, from apparently single-stranded precursors that arise from discrete genomic loci, termed piRNA clusters. Presently, little is known about the signals that distinguish a locus as a source of piRNAs. It is also unknown how individual piRNAs are selected from long precursor transcripts. To address these questions, we inserted new artificial sequence information into piRNA clusters and introduced these marked clusters as transgenes into heterologous genomic positions in mice and flies. Profiling of piRNA from transgenic animals demonstrated that artificial sequences were incorporated into the piRNA repertoire. Transgenic piRNA clusters are functional in non-native genomic contexts in both mice and flies, indicating that the signals that define piRNA generative loci must lie within the clusters themselves rather than being implicit in their genomic position. Comparison of transgenic animals that carry insertions of the same artificial sequence into different ectopic piRNA-generating loci showed that both local and long-range sequence environments inform the generation of individual piRNAs from precursor transcripts.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320338076163","@type":"Researcher","foaf:name":[{"@value":"Felix Muerdter"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320338076161","@type":"Researcher","foaf:name":[{"@value":"Ivan Olovnikov"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320338076166","@type":"Researcher","foaf:name":[{"@value":"Antoine Molaro"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320338076167","@type":"Researcher","foaf:name":[{"@value":"Nikolay V. Rozhkov"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320338076164","@type":"Researcher","foaf:name":[{"@value":"Benjamin Czech"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320338076162","@type":"Researcher","foaf:name":[{"@value":"Assaf Gordon"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320338076160","@type":"Researcher","foaf:name":[{"@value":"Gregory J. Hannon"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320338076165","@type":"Researcher","foaf:name":[{"@value":"Alexei A. Aravin"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13558382"},{"@type":"EISSN","@value":"14699001"}],"prism:publicationName":[{"@value":"RNA"}],"dc:publisher":[{"@value":"Cold Spring Harbor Laboratory"}],"prism:publicationDate":"2011-11-17","prism:volume":"18","prism:number":"1","prism:startingPage":"42","prism:endingPage":"52"},"reviewed":"false","url":[{"@id":"https://syndication.highwire.org/content/doi/10.1261/rna.029769.111"}],"createdAt":"2011-11-17","modifiedAt":"2021-11-21","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004232090498816","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Small RNAs: Artificial piRNAs for Transcriptional Silencing"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004232090525952","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Is the expression of sense and antisense transgenes really sufficient for artificial piRNA production?"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283692514623232","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Transcriptome profiling reveals infection strategy of an insect maculavirus"}]},{"@id":"https://cir.nii.ac.jp/crid/1360294643700313088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Potential for small RNA production against <i>Bombyx mori latent virus</i> in <i>Bombyx mori</i> ovaries"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565169112557056","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Hsp90 facilitates accurate loading of precursor piRNAs into PIWI proteins"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848657020628608","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Induction of DNA Methylation by Artificial piRNA Production in Male Germ Cells"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848661729315456","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"piRNA clusters and open chromatin structure"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848662035090560","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"A role for transcription from a piRNA cluster in de novo piRNA production"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848662035181696","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Artificial “ping-pong” cascade of PIWI-interacting RNA in silkworm cells"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1261/rna.029769.111"},{"@type":"CROSSREF","@value":"10.1016/j.cub.2015.01.060_references_DOI_S0Yp9U8c0K6l7DABxM8AxMDma6M"},{"@type":"CROSSREF","@value":"10.1093/dnares/dsx056_references_DOI_S0Yp9U8c0K6l7DABxM8AxMDma6M"},{"@type":"CROSSREF","@value":"10.1186/1759-8753-5-22_references_DOI_S0Yp9U8c0K6l7DABxM8AxMDma6M"},{"@type":"CROSSREF","@value":"10.1002/arch.21761_references_DOI_S0Yp9U8c0K6l7DABxM8AxMDma6M"},{"@type":"CROSSREF","@value":"10.1261/rna.058875.116_references_DOI_S0Yp9U8c0K6l7DABxM8AxMDma6M"},{"@type":"CROSSREF","@value":"10.1261/rna.037200.112_references_DOI_S0Yp9U8c0K6l7DABxM8AxMDma6M"},{"@type":"CROSSREF","@value":"10.1261/rna.029777.111_references_DOI_S0Yp9U8c0K6l7DABxM8AxMDma6M"},{"@type":"CROSSREF","@value":"10.1016/j.cub.2015.02.009_references_DOI_S0Yp9U8c0K6l7DABxM8AxMDma6M"},{"@type":"CROSSREF","@value":"10.1016/j.cub.2015.07.001_references_DOI_S0Yp9U8c0K6l7DABxM8AxMDma6M"}]}