{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320349027328.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1104/pp.111.1.243"}},{"identifier":{"@type":"URI","@value":"http://academic.oup.com/plphys/article-pdf/111/1/243/37085076/plphys_v111_1_243.pdf"}},{"identifier":{"@type":"NAID","@value":"30019361414"}}],"dc:title":[{"@value":"Transgenic AEQUORIN Reveals Organ-Specific Cytosolic Ca2+ Responses to Anoxia in Arabidopsis thaliana Seedlings"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n               <jats:p>Using the transgenic AEQUORIN system, we showed that the cotyledons and leaves of Arabidopsis thaliana seedlings developed a biphasic luminescence response to anoxia, indicating changes in cytosolic Ca2+ levels. A fast and transient luminescence peak occurred within minutes of anoxia, followed by a second, prolonged luminescence response that lasted 1.5 to 4 h. The Ca2+ channel blockers Gd3+, La3+, and ruthenium red (RR) partially inhibited the first response and promoted a larger and earlier second response, suggesting different origins for these responses. Both Gd3+ and RR also partially inhibited anaerobic induction of alcohol dehydrogenase gene expression. However, although anaerobic alcohol dehydrogenase gene induction occurred in seedlings exposed to water-agar medium and in roots, related luminescence responses were absent. Upon return to normoxia, the luminescence of cotyledons, leaves, and roots dropped quickly, before increasing again in a Gd3+-, La3+-, ethyleneglycol-bis(β-aminoethyl ether)-N,N′-tetraacetic acid-, and RR-sensitive fashion.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320349027332","@type":"Researcher","foaf:name":[{"@value":"J. C. Sedbrook"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Genetics, University of Wisconsin-Madison, 445 Henry Mall, Madison, Wisconsin 53706 (J.C.S., P.H.M.)"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320349027330","@type":"Researcher","foaf:name":[{"@value":"P. J. Kronebusch"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Genetics, University of Wisconsin-Madison, 445 Henry Mall, Madison, Wisconsin 53706 (J.C.S., P.H.M.)"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320349027331","@type":"Researcher","foaf:name":[{"@value":"G. G. Borisy"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Genetics, University of Wisconsin-Madison, 445 Henry Mall, Madison, Wisconsin 53706 (J.C.S., P.H.M.)"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320349027329","@type":"Researcher","foaf:name":[{"@value":"A. J. Trewavas"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Genetics, University of Wisconsin-Madison, 445 Henry Mall, Madison, Wisconsin 53706 (J.C.S., P.H.M.)"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320349027328","@type":"Researcher","foaf:name":[{"@value":"P. H. Masson"}],"jpcoar:affiliationName":[{"@value":"Laboratory of Genetics, University of Wisconsin-Madison, 445 Henry Mall, Madison, Wisconsin 53706 (J.C.S., P.H.M.)"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"15322548"},{"@type":"PISSN","@value":"00320889"}],"prism:publicationName":[{"@value":"Plant Physiology"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"1996-05-01","prism:volume":"111","prism:number":"1","prism:startingPage":"243","prism:endingPage":"257"},"reviewed":"false","dc:rights":["https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"],"url":[{"@id":"http://academic.oup.com/plphys/article-pdf/111/1/243/37085076/plphys_v111_1_243.pdf"}],"createdAt":"2002-07-27","modifiedAt":"2021-04-17","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390282681455819520","@type":"Article","relationType":["isReferencedBy","isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Hypoxia-Inducible Genes Encoding Small EF-Hand Proteins in Rice and Tomato"}]},{"@id":"https://cir.nii.ac.jp/crid/1522825129559411456","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"Evidence for the Involvement of Phospholipase C in the Anaerobic Signal Transduction"},{"@language":"ja-Kana","@value":"Evidence for the Involvement of Phospholipase C in the Anaerobic Signal Transduction"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1104/pp.111.1.243"},{"@type":"CIA","@value":"30019361414"},{"@type":"CROSSREF","@value":"10.1271/bbb.100549_references_DOI_UaPnwjvhYC3xz7HDBVOyAnAyMih"}]}