{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320452907648.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.0305476101"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.0305476101"}}],"dc:title":[{"@value":"Linear-After-The-Exponential (LATE)–PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n            Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the reaction annealing temperature. Linear-After-The-Exponential (LATE)–PCR describes a new paradigm for primer design that renders assays as efficient as symmetric PCR assays, regardless of primer ratio. LATE-PCR generates single-stranded products with predictable kinetics for many cycles beyond the exponential phase. LATE-PCR also introduces new probe design criteria that uncouple hybridization probe detection from primer annealing and extension, increase probe reliability, improve allele discrimination, and increase signal strength by 80–250% relative to symmetric PCR. These improvements in PCR are particularly useful for real-time quantitative analysis of target numbers in small samples. LATE-PCR is adaptable to high throughput applications in fields such as clinical diagnostics, biodefense, forensics, and DNA sequencing. We showcase LATE-PCR via amplification of the cystic fibrosis\n            <jats:italic>CF</jats:italic>\n            Δ\n            <jats:italic>508</jats:italic>\n            allele and the Tay-Sachs disease\n            <jats:italic>TSD 1278</jats:italic>\n            allele from single heterozygous cells.\n          </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320452907648","@type":"Researcher","foaf:name":[{"@value":"J. Aquiles Sanchez"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, MS 008, Brandeis University, 415 South Street, Waltham, MA 02454-9110"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320452907650","@type":"Researcher","foaf:name":[{"@value":"Kenneth E. Pierce"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, MS 008, Brandeis University, 415 South Street, Waltham, MA 02454-9110"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320452907651","@type":"Researcher","foaf:name":[{"@value":"John E. Rice"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, MS 008, Brandeis University, 415 South Street, Waltham, MA 02454-9110"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320452907649","@type":"Researcher","foaf:name":[{"@value":"Lawrence J. Wangh"}],"jpcoar:affiliationName":[{"@value":"Department of Biology, MS 008, Brandeis University, 415 South Street, Waltham, MA 02454-9110"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"2004-02-09","prism:volume":"101","prism:number":"7","prism:startingPage":"1933","prism:endingPage":"1938"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.0305476101"}],"createdAt":"2004-02-17","modifiedAt":"2022-04-12","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390851087355449728","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Rapid and Simple Detection of Isoniazid-Resistant <i>Mycobacterium tuberculosis</i> Utilizing a DNA Chromatography-Based Technique"},{"@value":"Rapid and Simple Detection of Isoniazid-Resistant Mycobacterium tuberculosis Utilizing a DNA Chromatography-Based Technique"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1073/pnas.0305476101"},{"@type":"CROSSREF","@value":"10.7883/yoken.jjid.2020.754_references_DOI_EuApsSX2QOWDgnCSjzJCzUicXAH"}]}