{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320531887360.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/mcb.24.14.6525-6535.2004"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/MCB.24.14.6525-6535.2004"}},{"identifier":{"@type":"URI","@value":"https://www.tandfonline.com/doi/pdf/10.1128/MCB.24.14.6525-6535.2004"}},{"identifier":{"@type":"PMID","@value":"15226451"}}],"dc:title":[{"@value":"Transcriptional Coactivator PC4 Stimulates Promoter Escape and Facilitates Transcriptional Synergy by GAL4-VP16"}],"description":[{"notation":[{"@value":"Positive cofactor 4 (PC4) is a coactivator that strongly augments transcription by various activators, presumably by facilitating the assembly of the preinitiation complex (PIC). However, our previous observation of stimulation of promoter escape in GAL4-VP16-dependent transcription in the presence of PC4 suggested a possible role for PC4 in this step. Here, we performed quantitative analyses of the stimulatory effects of PC4 on initiation, promoter escape, and elongation in GAL4-VP16-dependent transcription and found that PC4 possesses the ability to stimulate promoter escape in response to GAL4-VP16 in addition to its previously demonstrated effect on PIC assembly. This stimulatory effect of PC4 on promoter escape required TFIIA and the TATA box binding protein-associated factor subunits of TFIID. Furthermore, PC4 displayed physical interactions with both TFIIH and GAL4-VP16 through its coactivator domain, and these interactions were regulated distinctly by PC4 phosphorylation. Finally, GAL4-VP16 and PC4 stimulated both initiation and promoter escape to similar extents on the promoters with three and five GAL4 sites; however, they stimulated promoter escape preferentially on the promoter with a single GAL4 site. These results provide insight into the mechanism by which PC4 permits multiply bound GAL4-VP16 to attain synergy to achieve robust transcriptional activation."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320531887362","@type":"Researcher","foaf:name":[{"@value":"Aya Fukuda"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology, Saitama Medical School, Moroyama, Iruma-gun, Saitama 350-0495, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320531887367","@type":"Researcher","foaf:name":[{"@value":"Tomoyoshi Nakadai"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology, Saitama Medical School, Moroyama, Iruma-gun, Saitama 350-0495, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320531887361","@type":"Researcher","foaf:name":[{"@value":"Miho Shimada"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology, Saitama Medical School, Moroyama, Iruma-gun, Saitama 350-0495, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320531887365","@type":"Researcher","foaf:name":[{"@value":"Tohru Tsukui"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology, Saitama Medical School, Moroyama, Iruma-gun, Saitama 350-0495, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320531887366","@type":"Researcher","foaf:name":[{"@value":"Masahito Matsumoto"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology, Saitama Medical School, Moroyama, Iruma-gun, Saitama 350-0495, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320531887360","@type":"Researcher","foaf:name":[{"@value":"Yasuhisa Nogi"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology, Saitama Medical School, Moroyama, Iruma-gun, Saitama 350-0495, Japan"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320531887363","@type":"Researcher","foaf:name":[{"@value":"Michael Meisterernst"}],"jpcoar:affiliationName":[{"@value":"Gene Expression, Institute of Molecular Immunology, GSF-National Research Center for Environment and Health, Munich, Germany"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320531887364","@type":"Researcher","foaf:name":[{"@value":"Koji Hisatake"}],"jpcoar:affiliationName":[{"@value":"Department of Molecular Biology, Saitama Medical School, Moroyama, Iruma-gun, Saitama 350-0495, Japan"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"10985549"}],"prism:publicationName":[{"@value":"Molecular and Cellular Biology"}],"dc:publisher":[{"@value":"Informa UK Limited"}],"prism:publicationDate":"2004-07-01","prism:volume":"24","prism:number":"14","prism:startingPage":"6525","prism:endingPage":"6535"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/MCB.24.14.6525-6535.2004"},{"@id":"https://www.tandfonline.com/doi/pdf/10.1128/MCB.24.14.6525-6535.2004"}],"createdAt":"2004-06-29","modifiedAt":"2023-03-28","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Models,%20Genetic","dc:title":"Models, Genetic"},{"@id":"https://cir.nii.ac.jp/all?q=Transcription,%20Genetic","dc:title":"Transcription, Genetic"},{"@id":"https://cir.nii.ac.jp/all?q=Repressor%20Proteins","dc:title":"Repressor Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Protein%20Subunits","dc:title":"Protein Subunits"},{"@id":"https://cir.nii.ac.jp/all?q=Gene%20Expression%20Regulation","dc:title":"Gene Expression Regulation"},{"@id":"https://cir.nii.ac.jp/all?q=Trans-Activators","dc:title":"Trans-Activators"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Promoter%20Regions,%20Genetic","dc:title":"Promoter Regions, Genetic"},{"@id":"https://cir.nii.ac.jp/all?q=Transcription%20Factors","dc:title":"Transcription Factors"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001202618561408","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Heterogeneous Nuclear Ribonucleoprotein R Cooperates with Mediator to Facilitate Transcription Reinitiation on the c-Fos Gene"}]},{"@id":"https://cir.nii.ac.jp/crid/1050001202633751296","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"The RNA Binding Complexes NF45-NF90 and NF45-NF110 Associate Dynamically with the c-fos Gene and Function as Transcriptional Coactivators"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285710497801472","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Transcription cofactor <scp>PC</scp>4 plays essential roles in collaboration with the small subunit of general transcription factor <scp>TFIIE</scp>"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/mcb.24.14.6525-6535.2004"},{"@type":"OPENAIRE","@value":"doi_dedup___::8bc9bd67a5d2c3cc16887fad5512e9ef"},{"@type":"CROSSREF","@value":"10.1074/jbc.m115.688317_references_DOI_5Efzdy329fXA96IAhADtBWh4AKs"},{"@type":"CROSSREF","@value":"10.1111/gtc.12187_references_DOI_5Efzdy329fXA96IAhADtBWh4AKs"},{"@type":"CROSSREF","@value":"10.1371/journal.pone.0072496_references_DOI_5Efzdy329fXA96IAhADtBWh4AKs"}]}