{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320677316224.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/anie.201907460"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fanie.201907460"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.201907460"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/anie.201907460"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/am-pdf/10.1002/anie.201907460"}}],"dc:title":[{"@value":"Site‐Selective Functionalization of (sp<sup>3</sup>)C−H Bonds Catalyzed by Artificial Metalloenzymes Containing an Iridium‐Porphyrin Cofactor"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p>The selective functionalization of one C−H bond over others in nearly identical steric and electronic environments can facilitate the construction of complex molecules. We report site‐selective functionalizations of C−H bonds, differentiated solely by remote substituents, catalyzed by artificial metalloenzymes (ArMs) that are generated from the combination of an evolvable P450 scaffold and an iridium‐porphyrin cofactor. The generated systems catalyze the insertion of carbenes into the C−H bonds of a range of phthalan derivatives containing substituents that render the two methylene positions in each phthalan inequivalent. These reactions occur with site‐selectivity ratios of up to 17.8:1 and, in most cases, with pairs of enzyme mutants that preferentially form each of the two constitutional isomers. This study demonstrates the potential of abiotic reactions catalyzed by metalloenzymes to functionalize C−H bonds with site selectivity that is difficult to achieve with small‐molecule catalysts.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320677316227","@type":"Researcher","foaf:name":[{"@value":"Yang Gu"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry University of California  Berkeley CA 94720 USA"},{"@value":"Chemical Sciences Division Lawrence Berkeley National Laboratory  1 Cyclotron Road Berkeley CA 94720 USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320677316225","@type":"Researcher","foaf:name":[{"@value":"Sean N. Natoli"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry University of California  Berkeley CA 94720 USA"},{"@value":"Chemical Sciences Division Lawrence Berkeley National Laboratory  1 Cyclotron Road Berkeley CA 94720 USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320677316224","@type":"Researcher","foaf:name":[{"@value":"Zhennan Liu"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry University of California  Berkeley CA 94720 USA"},{"@value":"Chemical Sciences Division Lawrence Berkeley National Laboratory  1 Cyclotron Road Berkeley CA 94720 USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320677316226","@type":"Researcher","foaf:name":[{"@value":"Douglas S. Clark"}],"jpcoar:affiliationName":[{"@value":"Department of Chemical and Biomolecular Engineering University of California  Berkeley CA 94720 USA"},{"@value":"Molecular Biophysics and Integrated Bioimaging Division Lawrence Berkeley National Laboratory  1 Cyclotron Road Berkeley CA 94720 USA"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320677316228","@type":"Researcher","foaf:name":[{"@value":"John F. Hartwig"}],"jpcoar:affiliationName":[{"@value":"Department of Chemistry University of California  Berkeley CA 94720 USA"},{"@value":"Chemical Sciences Division Lawrence Berkeley National Laboratory  1 Cyclotron Road Berkeley CA 94720 USA"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"14337851"},{"@type":"EISSN","@value":"15213773"}],"prism:publicationName":[{"@value":"Angewandte Chemie International Edition"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2019-08-21","prism:volume":"58","prism:number":"39","prism:startingPage":"13954","prism:endingPage":"13960"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#am","http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fanie.201907460"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.201907460"},{"@id":"https://onlinelibrary.wiley.com/doi/full-xml/10.1002/anie.201907460"},{"@id":"https://onlinelibrary.wiley.com/doi/am-pdf/10.1002/anie.201907460"}],"createdAt":"2019-07-29","modifiedAt":"2023-09-09","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360572092711540736","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Artificial Metalloenzymes: From Selective Chemical Transformations to Biochemical Applications"}]},{"@id":"https://cir.nii.ac.jp/crid/2050870367081136768","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Exploring and adapting the molecular selectivity of artificial metalloenzymes"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/anie.201907460"},{"@type":"CROSSREF","@value":"10.1246/bcsj.20200316_references_DOI_AtD80uhACngpjXLAyfniGa9dckF"},{"@type":"CROSSREF","@value":"10.3390/molecules25132989_references_DOI_AtD80uhACngpjXLAyfniGa9dckF"}]}