{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320692246272.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1128/jvi.70.11.8098-8108.1996"}},{"identifier":{"@type":"URI","@value":"https://journals.asm.org/doi/pdf/10.1128/jvi.70.11.8098-8108.1996"}}],"dc:title":[{"@value":"Efficient long-term gene transfer into muscle tissue of immunocompetent mice by adeno-associated virus vector"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Muscle-directed gene transfer is being considered for the treatment of several metabolic diseases, including hemophilia and Duchene's muscular dystrophy. Previous efforts to target this tissue for somatic delivery with various vector systems have resulted in transient expression due to silencing of the transgene or to an immune response against the vector-transduced cells. We introduced recombinant adeno-associated virus vector (rAAV) carrying a lacZ reporter into muscle tissue of immunocompetent mice. The lacZ reporter gene was efficiently transduced and expressed with no evidence of a cellular immune response. Moreover, gene expression persisted for more than 1.5 years. Molecular characterization of rAAV vector DNA suggests a mechanism for persistence, since vector episomes convert to high-molecular-weight genomic DNA. These data provide the first report for establishing long-term gene transduction into mammalian muscle cells in vivo without the need for immune modulation of the organism.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380567187830927872","@type":"Researcher","foaf:name":[{"@value":"X Xiao"}],"jpcoar:affiliationName":[{"@value":"Gene Therapy Center, University of North Carolina at Chapel Hill, 27599, USA."}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320692246273","@type":"Researcher","foaf:name":[{"@value":"J Li"}],"jpcoar:affiliationName":[{"@value":"Gene Therapy Center, University of North Carolina at Chapel Hill, 27599, USA."}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320692246274","@type":"Researcher","foaf:name":[{"@value":"R J Samulski"}],"jpcoar:affiliationName":[{"@value":"Gene Therapy Center, University of North Carolina at Chapel Hill, 27599, USA."}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0022538X"},{"@type":"EISSN","@value":"10985514"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/0022538X"}],"prism:publicationName":[{"@value":"Journal of Virology"}],"dc:publisher":[{"@value":"American Society for Microbiology"}],"prism:publicationDate":"1996-11","prism:volume":"70","prism:number":"11","prism:startingPage":"8098","prism:endingPage":"8108"},"reviewed":"false","dc:rights":["https://journals.asm.org/non-commercial-tdm-license"],"url":[{"@id":"https://journals.asm.org/doi/pdf/10.1128/jvi.70.11.8098-8108.1996"}],"createdAt":"2020-01-06","modifiedAt":"2022-03-05","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050870696494010880","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Adeno-Associated Virus as an Effective Malaria Booster Vaccine Following Adenovirus Priming"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283691773989632","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Protein-anchoring Strategy for Delivering Acetylcholinesterase to the Neuromuscular Junction"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285706978381568","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Improved transduction efficiencies of adeno-associated virus vectors by synthetic cell-permeable peptides"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285709455846144","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Protein-Anchoring Therapy of Biglycan for\n                    <i>Mdx</i>\n                    Mouse Model of Duchenne Muscular Dystrophy"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567187830927744","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Leptin gene therapy in the fight against diabetes"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656438107264","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"An Adeno-Associated Virus Vector Efficiently and Specifically Transduces Mouse Skeletal Muscle"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282679249985024","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"A new paradigm for the progression of advanced heart failure."},{"@language":"ja","@value":"心不全の重症化機構－その新しい概念－"},{"@language":"ja-Kana","@value":"シンフゼン ノ ジュウショウカ キコウ ソノ アタラシイ ガイネン"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1128/jvi.70.11.8098-8108.1996"},{"@type":"CROSSREF","@value":"10.1038/mt.2012.34_references_DOI_Y4A3BW77nhMVzACaiJ55rIJwXGQ"},{"@type":"CROSSREF","@value":"10.1016/j.bbrc.2016.09.014_references_DOI_Y4A3BW77nhMVzACaiJ55rIJwXGQ"},{"@type":"CROSSREF","@value":"10.1517/14712598.2010.512286_references_DOI_W32PZaUHdfJaTI1DyobJ6UPnldk"},{"@type":"CROSSREF","@value":"10.1254/fpj.123.55_references_DOI_W32PZaUHdfJaTI1DyobJ6UPnldk"},{"@type":"CROSSREF","@value":"10.1089/hum.2015.088_references_DOI_Y4A3BW77nhMVzACaiJ55rIJwXGQ"},{"@type":"CROSSREF","@value":"10.3389/fimmu.2019.00730_references_DOI_Y4A3BW77nhMVzACaiJ55rIJwXGQ"},{"@type":"CROSSREF","@value":"10.1007/s12033-010-9369-z_references_DOI_W32PZaUHdfJaTI1DyobJ6UPnldk"}]}