{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320732264704.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1038/s41467-018-07224-8"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41467-018-07224-8.pdf"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41467-018-07224-8"}}],"dc:title":[{"@value":"Transcriptome-wide identification of transient RNA G-quadruplexes in human cells"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n                  <jats:p>Guanine-rich RNA sequences can fold into four-stranded structures, termed G-quadruplexes (G4-RNAs), whose biological roles are poorly understood, and in vivo existence is debated. To profile biologically relevant G4-RNA in the human transcriptome, we report here on G4RP-seq, which combines G4-RNA-specific precipitation (G4RP) with sequencing. This protocol comprises a chemical crosslinking step, followed by affinity capture with the G4-specific small-molecule ligand/probe BioTASQ, and target identification by sequencing, allowing for capturing global snapshots of transiently folded G4-RNAs. We detect widespread G4-RNA targets within the transcriptome, indicative of transient G4 formation in living human cells. Using G4RP-seq, we also demonstrate that G4-stabilizing ligands (BRACO-19 and RHPS4) can change the G4 transcriptomic landscape, most notably in long non-coding RNAs. G4RP-seq thus provides a method for studying the G4-RNA landscape, as well as ways of considering the mechanisms underlying G4-RNA formation, and the activity of G4-stabilizing ligands.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320732264579","@type":"Researcher","foaf:name":[{"@value":"Sunny Y. Yang"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320732264578","@type":"Researcher","foaf:name":[{"@value":"Pauline Lejault"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320732264705","@type":"Researcher","foaf:name":[{"@value":"Sandy Chevrier"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320732264576","@type":"Researcher","foaf:name":[{"@value":"Romain Boidot"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320732264577","@type":"Researcher","foaf:name":[{"@value":"A. Gordon Robertson"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320732264704","@type":"Researcher","foaf:name":[{"@value":"Judy M. Y. Wong"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320732264706","@type":"Researcher","foaf:name":[{"@value":"David Monchaud"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"20411723"}],"prism:publicationName":[{"@value":"Nature Communications"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2018-11-09","prism:volume":"9","prism:number":"1","prism:startingPage":"4730"},"reviewed":"false","dc:rights":["https://creativecommons.org/licenses/by/4.0","https://creativecommons.org/licenses/by/4.0"],"url":[{"@id":"https://www.nature.com/articles/s41467-018-07224-8.pdf"},{"@id":"https://www.nature.com/articles/s41467-018-07224-8"}],"createdAt":"2018-11-05","modifiedAt":"2022-12-20","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050004225395312384","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Modulation of histone 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