{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320768633088.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1016/0014-5793(87)80761-5"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2887%2980761-5"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(87)80761-5"}},{"identifier":{"@type":"URI","@value":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2887%2980761-5"}}],"dc:title":[{"@value":"Role of acidic phosphoproteins in the partial reconstitution of the active 60 S ribosomal subunit"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>We have recently shown that rat liver 60 S ribosomal subunits active in protein synthesis can be reconstituted from inactive core particles lacking 30% of the total proteins, mainly L10a, L12, L22, L24, A33 and the acidic phosphoproteins P1–P2, obtained by treatment of 60 S subunits with dimethylmaleic anhydride [(1987) Eur. J. Biochem. 163, 15–20]. In this study, an ethanol extract of the 60 S subunit which contains only P1–P2 was also shown to be effective in reconstitution with the DMMA‐core‐particies: it strongly stimulated the EF‐2‐dependent GTP hydrolysis and, to a lesser extent, polyphenylalanine synthesis; like the DMMA wash it shifted the thermal denaturation curve of the DMMA‐core particles towards that of control subunits. Prior dephosphorylation of the ethanol extract by alkaline phosphatase inhibited the reconstruction process.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320768633090","@type":"Researcher","foaf:name":[{"@value":"Jean-Pierre Lavergne"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320768633089","@type":"Researcher","foaf:name":[{"@value":"François Conquet"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320768633088","@type":"Researcher","foaf:name":[{"@value":"Jean-Paul Reboud"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320768633091","@type":"Researcher","foaf:name":[{"@value":"Anne-Marie Reboud"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00145793"},{"@type":"EISSN","@value":"18733468"}],"prism:publicationName":[{"@value":"FEBS Letters"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"1987-05-25","prism:volume":"216","prism:number":"1","prism:startingPage":"83","prism:endingPage":"88"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2887%2980761-5"},{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(87)80761-5"},{"@id":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2887%2980761-5"}],"createdAt":"2002-07-25","modifiedAt":"2023-09-16","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360567183206825856","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Proteomic Analysis of Rodent Ribosomes Revealed Heterogeneity Including Ribosomal Proteins L10-like, L22-like 1, and L39-like"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1016/0014-5793(87)80761-5"},{"@type":"CROSSREF","@value":"10.1021/pr9008964_references_DOI_GgglDBmmDBVblRCRffmNq70ZjxS"}]}