{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320790093056.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1093/jaoac/75.5.829"}},{"identifier":{"@type":"URI","@value":"http://academic.oup.com/jaoac/article-pdf/75/5/829/32455041/jaoac0829.pdf"}}],"dc:title":[{"@value":"Liquid Chromatographic Determination of Fumonisins with 4-Fluoro-7-nitrobenzofurazan"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n               <jats:p>Fumonisins B1 and B2 are closely related mycotoxins produced by Fusarium moniliforme, F. proliferatum, and related species. Disadvantages of 2 fluorescence derivatizing reagents currently used for liquid chromatography (LC) are instability of the derivatives (with o-phthaldialdehyde-mercaptoethanol) and formation of 2 peaks (with fluorescamine). 4-Fluoro-7-nitrobenzofurazan (4-fluoro-7- nitrobenz-2-oxa-1,3-diazole; NBD-F) was, therefore, investigated. Although a larger molar excess of this reagent is required for fumonisins than for amino acids, the derivatives formed are moderately stable, and as little as 1 ng fumonisins B1 or B2 can be detected, with a linear response up to at least 50 ng injected. Reversed-phase LC (C18 column) was carried out with a mobile phase of methanol- 0.05M sodium dihydrogen phosphate adjusted to pH 5 (1 -1-1), which was mixed with an equal volume of acetonitrile-water (8 + 2) after 5 min. Using a modification of a strong anion exchange cleanup procedure, good recoveries (averages of 94 and 80%, respectively) of fumonisins B1 and B2 from ground corn, corn meal, and corn flakes in the 125̶ 5000 ng/g range were generally obtained; the limit of detection of the overall method was about100 ng/g. Similar results were obtained in studies with ground corn, corn meal, and corn flakes using naphthalene-2,3-dicarboxaldehyde-potassium cyanide (NDA-KCN) for derivatization; average recoveries of fumonisins B1 and B2 were 94 and 83%, respectively.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320790093057","@type":"Researcher","foaf:name":[{"@value":"Peter M Scott"}],"jpcoar:affiliationName":[{"@value":"Health and Welfare Canada, Health Protection Branch, Food Directorate, Bureau of Chemical Safety, Food Research Division, Ottawa, ON, KlA 0L2, Canada"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320790093056","@type":"Researcher","foaf:name":[{"@value":"Guillaume A Lawrence"}],"jpcoar:affiliationName":[{"@value":"Health and Welfare Canada, Health Protection Branch, Food Directorate, Bureau of Chemical Safety, Food Research Division, Ottawa, ON, KlA 0L2, Canada"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"10603271"},{"@type":"EISSN","@value":"19447922"},{"@type":"PISSN","@value":"http://id.crossref.org/issn/10603271"}],"prism:publicationName":[{"@value":"Journal of AOAC INTERNATIONAL"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"1992-09-01","prism:volume":"75","prism:number":"5","prism:startingPage":"829","prism:endingPage":"833"},"reviewed":"false","dc:rights":["https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"],"url":[{"@id":"http://academic.oup.com/jaoac/article-pdf/75/5/829/32455041/jaoac0829.pdf"}],"createdAt":"2020-02-14","modifiedAt":"2020-03-19","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1390282679200599936","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Analysis of Fumonisin B1 and B2 in Beer and Raw Materials of Beer by Liquid Chromatography/Tandem Mass Spectrometry"},{"@language":"ja","@value":"ＬＣ／ＭＳ／ＭＳによるビールおよびビール原料中フモニシンＢ１およびＢ２の分析"},{"@language":"ja-Kana","@value":"LC MS MS ニ ヨル ビール オヨビ ビール ゲンリョウ チュウ フモニシン B1 オヨビ B2 ノ ブンセキ"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1093/jaoac/75.5.829"},{"@type":"CROSSREF","@value":"10.3358/shokueishi.45.255_references_DOI_AdU3JsgSTcU7LyryQnvyUpmeK2L"}]}