{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320877549568.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1083/jcb.147.7.1569"}},{"identifier":{"@type":"URI","@value":"https://rupress.org/jcb/article-pdf/147/7/1569/1855411/9908015.pdf"}}],"dc:title":[{"@value":"Cingulin Contains Globular and Coiled-Coil Domains and Interacts with Zo-1, Zo-2, Zo-3, and Myosin"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>We characterized the sequence and protein interactions of cingulin, an Mr 140–160-kD phosphoprotein localized on the cytoplasmic surface of epithelial tight junctions (TJ). The derived amino acid sequence of a full-length Xenopus laevis cingulin cDNA shows globular head (residues 1–439) and tail (1,326–1,368) domains and a central α-helical rod domain (440–1,325). Sequence analysis, electron microscopy, and pull-down assays indicate that the cingulin rod is responsible for the formation of coiled-coil parallel dimers, which can further aggregate through intermolecular interactions. Pull-down assays from epithelial, insect cell, and reticulocyte lysates show that an NH2-terminal fragment of cingulin (1–378) interacts in vitro with ZO-1 (Kd ∼5 nM), ZO-2, ZO-3, myosin, and AF-6, but not with symplekin, and a COOH-terminal fragment (377–1,368) interacts with myosin and ZO-3. ZO-1 and ZO-2 immunoprecipitates contain cingulin, suggesting in vivo interactions. Full-length cingulin, but not NH2-terminal and COOH-terminal fragments, colocalizes with endogenous cingulin in transfected MDCK cells, indicating that sequences within both head and rod domains are required for TJ localization. We propose that cingulin is a functionally important component of TJ, linking the submembrane plaque domain of TJ to the actomyosin cytoskeleton.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320877549570","@type":"Researcher","foaf:name":[{"@value":"Michelangelo Cordenonsi"}],"jpcoar:affiliationName":[{"@value":"aDepartment of Biology, University of Padova, 35121 Padova, Italy"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320877549569","@type":"Researcher","foaf:name":[{"@value":"Fabio D'Atri"}],"jpcoar:affiliationName":[{"@value":"bDepartment of Biochemistry, University of Geneva, 1211 Geneva 4, Switzerland"},{"@value":"cDepartment of Molecular Biology, University of Geneva, 1211 Geneva 4, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320877549571","@type":"Researcher","foaf:name":[{"@value":"Eva Hammar"}],"jpcoar:affiliationName":[{"@value":"cDepartment of Molecular Biology, University of Geneva, 1211 Geneva 4, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320877549574","@type":"Researcher","foaf:name":[{"@value":"David A.D. Parry"}],"jpcoar:affiliationName":[{"@value":"dInstitute of Fundamental Sciences, Massey University, Palmerston North, New Zealand"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320877549568","@type":"Researcher","foaf:name":[{"@value":"John Kendrick-Jones"}],"jpcoar:affiliationName":[{"@value":"eMedical Research Council Laboratory of Molecular Biology, Cambridge, CB22QH UK"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320877549572","@type":"Researcher","foaf:name":[{"@value":"David Shore"}],"jpcoar:affiliationName":[{"@value":"cDepartment of Molecular Biology, University of Geneva, 1211 Geneva 4, Switzerland"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320877549573","@type":"Researcher","foaf:name":[{"@value":"Sandra Citi"}],"jpcoar:affiliationName":[{"@value":"aDepartment of Biology, University of Padova, 35121 Padova, Italy"},{"@value":"cDepartment of Molecular Biology, University of Geneva, 1211 Geneva 4, Switzerland"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00219525"},{"@type":"EISSN","@value":"15408140"}],"prism:publicationName":[{"@value":"The Journal of Cell Biology"}],"dc:publisher":[{"@value":"Rockefeller University Press"}],"prism:publicationDate":"1999-12-27","prism:volume":"147","prism:number":"7","prism:startingPage":"1569","prism:endingPage":"1582"},"reviewed":"false","url":[{"@id":"https://rupress.org/jcb/article-pdf/147/7/1569/1855411/9908015.pdf"}],"createdAt":"2002-07-26","modifiedAt":"2023-07-29","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360017282434371456","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Inhibition of HDAC and Signal Transduction Pathways Induces Tight Junctions and Promotes Differentiation in p63-Positive Salivary Duct Adenocarcinoma"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283693327528320","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Apical cytoskeletons and junctional complexes as a combined system in epithelial cell sheets"}]},{"@id":"https://cir.nii.ac.jp/crid/1360290617773622912","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"E74-Like Factor 3 Is a Key Regulator of Epithelial Integrity and Immune Response Genes in Biliary Tract Cancer"}]},{"@id":"https://cir.nii.ac.jp/crid/1360588381051444224","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The roles of tight junction protein cingulin in human endometrioid endometrial cancer"}]},{"@id":"https://cir.nii.ac.jp/crid/1360846640183726208","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Regulation of intestinal epithelial permeability by tight junctions"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848658419225984","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"AMPK-dependent phosphorylation of cingulin reversibly regulates its binding to actin filaments and microtubules"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848663203219072","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Frequent <i>CLDN18-ARHGAP</i> fusion in highly metastatic diffuse-type gastric cancer with relatively early onset"}]},{"@id":"https://cir.nii.ac.jp/crid/1360853567390319872","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Pyk2 inhibitor prevents epithelial hyperpermeability induced by HMGB1 and inflammatory cytokines in Caco-2 cells"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1083/jcb.147.7.1569"},{"@type":"CROSSREF","@value":"10.3390/cancers14112584_references_DOI_aIrb7AStE9SjAn4ok8prKRwHaI5"},{"@type":"CROSSREF","@value":"10.1111/nyas.13432_references_DOI_aIrb7AStE9SjAn4ok8prKRwHaI5"},{"@type":"CROSSREF","@value":"10.1158/0008-5472.can-19-2988_references_DOI_aIrb7AStE9SjAn4ok8prKRwHaI5"},{"@type":"CROSSREF","@value":"10.1080/21688370.2024.2361976_references_DOI_aIrb7AStE9SjAn4ok8prKRwHaI5"},{"@type":"CROSSREF","@value":"10.1007/s00018-012-1070-x_references_DOI_aIrb7AStE9SjAn4ok8prKRwHaI5"},{"@type":"CROSSREF","@value":"10.1038/s41598-018-33418-7_references_DOI_aIrb7AStE9SjAn4ok8prKRwHaI5"},{"@type":"CROSSREF","@value":"10.18632/oncotarget.25464_references_DOI_aIrb7AStE9SjAn4ok8prKRwHaI5"},{"@type":"CROSSREF","@value":"10.1080/21688370.2021.1890526_references_DOI_aIrb7AStE9SjAn4ok8prKRwHaI5"}]}