{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320897225472.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/(sici)1098-2280(1999)34:1<1::aid-em1>3.0.co;2-p"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2F(SICI)1098-2280(1999)34:1%3C1::AID-EM1%3E3.0.CO;2-P"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/full/10.1002/(SICI)1098-2280(1999)34:1%3C1::AID-EM1%3E3.0.CO;2-P"}},{"identifier":{"@type":"PMID","@value":"10462717"}}],"dc:title":[{"@value":"Spectra ofgpt mutations in ethylnitrosourea-treated and untreated transgenic mice"}],"description":[{"notation":[{"@value":"We have established a new transgenic mouse mutagenicity assay for the efficient detection of point mutations and deletions in vivo (Nohmi et al. [1996] Env. Mol. Mutagen. 28:465-470). In this assay, the gpt gene of Escherichia coli is used as a reporter for the detection of point mutations. Treatment of mice with ethylnitrosourea (ENU, 150 mg/kg) enhances by several-fold the mutant frequency of gpt in bone marrow. Here, we report the mutation spectra of the gpt gene recovered from bone marrow of ENU-treated and untreated transgenic mice. In the gpt mutants rescued from ENU-treated mice, more than 90% of the mutations were base change mutations; the predominant types were A:T to T:A transversions and G:C to A:T transitions. On the contrary, in the mutants rescued from untreated mice, 54% were base substitutions and the remainders were short deletions and insertions. Among untreated mice, the most frequently observed base substitution was G:C to A:T transitions (7/14 mutants). Three of these occurred at 5'-CpG-3' sites. Interestingly, the mutation spectra of the gpt gene were different from those of the gpt gene in ENU-treated and untreated E.coli, whereas they were similar to those of the lacZ and lacI genes in ENU-treated and untreated other transgenic mice or cultured mammalian cells. We also report the establishment of homozygous transgenic mice that have transgene lambdaEG10 DNA in both chromosome 17 of C57BL/6J mouse."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380004057904408591","@type":"Researcher","foaf:name":[{"@value":"K. Masumura"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320897225478","@type":"Researcher","foaf:name":[{"@value":"M. Matsui"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320897225477","@type":"Researcher","foaf:name":[{"@value":"M. Katoh"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320897225481","@type":"Researcher","foaf:name":[{"@value":"N. Horiya"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320897225480","@type":"Researcher","foaf:name":[{"@value":"O. Ueda"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320897225475","@type":"Researcher","foaf:name":[{"@value":"H. Tanabe"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320897225476","@type":"Researcher","foaf:name":[{"@value":"M. Yamada"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320897225479","@type":"Researcher","foaf:name":[{"@value":"H. Suzuki"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320897225474","@type":"Researcher","foaf:name":[{"@value":"T. Sofuni"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320897225473","@type":"Researcher","foaf:name":[{"@value":"T. Nohmi"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"08936692"},{"@type":"EISSN","@value":"10982280"}],"prism:publicationName":[{"@value":"Environmental and Molecular Mutagenesis"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"1999","prism:volume":"34","prism:number":"1","prism:startingPage":"1","prism:endingPage":"8"},"reviewed":"false","dc:rights":["http://doi.wiley.com/10.1002/tdm_license_1.1"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2F(SICI)1098-2280(1999)34:1%3C1::AID-EM1%3E3.0.CO;2-P"},{"@id":"https://onlinelibrary.wiley.com/doi/full/10.1002/(SICI)1098-2280(1999)34:1%3C1::AID-EM1%3E3.0.CO;2-P"}],"createdAt":"2002-08-25","modifiedAt":"2021-07-01","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Base%20Sequence","dc:title":"Base Sequence"},{"@id":"https://cir.nii.ac.jp/all?q=Mutagenicity%20Tests","dc:title":"Mutagenicity Tests"},{"@id":"https://cir.nii.ac.jp/all?q=Escherichia%20coli%20Proteins","dc:title":"Escherichia coli Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Homozygote","dc:title":"Homozygote"},{"@id":"https://cir.nii.ac.jp/all?q=Molecular%20Sequence%20Data","dc:title":"Molecular Sequence Data"},{"@id":"https://cir.nii.ac.jp/all?q=Chromosome%20Mapping","dc:title":"Chromosome Mapping"},{"@id":"https://cir.nii.ac.jp/all?q=Proteins","dc:title":"Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Transgenic","dc:title":"Mice, Transgenic"},{"@id":"https://cir.nii.ac.jp/all?q=Bacteriophage%20lambda","dc:title":"Bacteriophage lambda"},{"@id":"https://cir.nii.ac.jp/all?q=Chromosomes","dc:title":"Chromosomes"},{"@id":"https://cir.nii.ac.jp/all?q=Chromosome%20Banding","dc:title":"Chromosome Banding"},{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Inbred%20C57BL","dc:title":"Mice, Inbred C57BL"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Bacterial%20Proteins","dc:title":"Bacterial Proteins"},{"@id":"https://cir.nii.ac.jp/all?q=Bone%20Marrow","dc:title":"Bone Marrow"},{"@id":"https://cir.nii.ac.jp/all?q=Ethylnitrosourea","dc:title":"Ethylnitrosourea"},{"@id":"https://cir.nii.ac.jp/all?q=Mutation","dc:title":"Mutation"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Point%20Mutation","dc:title":"Point Mutation"},{"@id":"https://cir.nii.ac.jp/all?q=Female","dc:title":"Female"},{"@id":"https://cir.nii.ac.jp/all?q=Pentosyltransferases","dc:title":"Pentosyltransferases"},{"@id":"https://cir.nii.ac.jp/all?q=In%20Situ%20Hybridization,%20Fluorescence","dc:title":"In Situ Hybridization, Fluorescence"},{"@id":"https://cir.nii.ac.jp/all?q=Mutagens","dc:title":"Mutagens"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285706146641152","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The application of hepatic P450 reductase null gpt delta mice in studying the role of hepatic P450 in genotoxic carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced mutagenesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1360285709741534720","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Integration of In Vivo Genotoxicity and Short-term Carcinogenicity Assays Using F344 gpt Delta Transgenic Rats: In Vivo Mutagenicity of 2,4-Diaminotoluene and 2,6-Diaminotoluene Structural Isomers"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567180060660864","@type":"Article","resourceType":"学術雑誌論文(journal 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