{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320942667264.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1126/science.1076184"}},{"identifier":{"@type":"URI","@value":"https://www.science.org/doi/pdf/10.1126/science.1076184"}},{"identifier":{"@type":"NAID","@value":"30020382239"}}],"dc:title":[{"@value":"Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Tomography"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>\n                    Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We applied this technique to\n                    <jats:italic>Dictyostelium</jats:italic>\n                    cells, focusing on the actin cytoskeleton. In actin networks reconstructed without prior removal of membranes or extraction of soluble proteins, the cross-linking of individual microfilaments, their branching angles, and membrane attachment sites can be analyzed. At a resolution of 5 to 6 nanometers, single macromolecules with distinct shapes, such as the 26\n                    <jats:italic>S</jats:italic>\n                    proteasome, can be identified in an unperturbed cellular environment.\n                  </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320942667136","@type":"Researcher","foaf:name":[{"@value":"Ohad Medalia"}],"jpcoar:affiliationName":[{"@value":"Max Planck Institute for Biochemistry, D-82152 Martinsried, Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320942667264","@type":"Researcher","foaf:name":[{"@value":"Igor Weber"}],"jpcoar:affiliationName":[{"@value":"Max Planck Institute for Biochemistry, D-82152 Martinsried, Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320942667137","@type":"Researcher","foaf:name":[{"@value":"Achilleas S. Frangakis"}],"jpcoar:affiliationName":[{"@value":"Max Planck Institute for Biochemistry, D-82152 Martinsried, Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320942667266","@type":"Researcher","foaf:name":[{"@value":"Daniela Nicastro"}],"jpcoar:affiliationName":[{"@value":"Max Planck Institute for Biochemistry, D-82152 Martinsried, Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320942667138","@type":"Researcher","foaf:name":[{"@value":"Günther Gerisch"}],"jpcoar:affiliationName":[{"@value":"Max Planck Institute for Biochemistry, D-82152 Martinsried, Germany."}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320942667265","@type":"Researcher","foaf:name":[{"@value":"Wolfgang Baumeister"}],"jpcoar:affiliationName":[{"@value":"Max Planck Institute for Biochemistry, D-82152 Martinsried, Germany."}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00368075"},{"@type":"EISSN","@value":"10959203"}],"prism:publicationName":[{"@value":"Science"}],"dc:publisher":[{"@value":"American Association for the Advancement of Science (AAAS)"}],"prism:publicationDate":"2002-11-08","prism:volume":"298","prism:number":"5596","prism:startingPage":"1209","prism:endingPage":"1213"},"reviewed":"false","url":[{"@id":"https://www.science.org/doi/pdf/10.1126/science.1076184"}],"createdAt":"2002-11-07","modifiedAt":"2024-01-09","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050001335841232512","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane"}]},{"@id":"https://cir.nii.ac.jp/crid/1050283688375732736","@type":"Article","resourceType":"学術雑誌論文(journal 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