{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320964589056.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.0915022107"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.0915022107"}}],"dc:title":[{"@value":"miRNA-based mechanism for the commitment of multipotent progenitors to a single cellular fate"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>When stem cells and multipotent progenitors differentiate, they undergo fate restriction, enabling a single fate and blocking differentiation along alternative routes. We herein present a mechanism whereby such unequivocal commitment is achieved, based on microRNA (miRNA)-dependent repression of an alternative cell fate. We show that the commitment of monocyte RAW264.7 progenitors to active macrophage differentiation involves rapid up-regulation of miR-155 expression, which leads to the suppression of the alternative pathway, namely RANK ligand-induced osteoclastogenesis, by repressing the expression of MITF, a transcription factor essential for osteoclast differentiation. A temporal asymmetry, whereby miR-155 expression precedes and overrides the activation of the osteoclast transcriptional program, provides the means for coherent macrophage differentiation, even in the presence of osteoclastogenic signals. Based on these findings, we propose that miRNA may provide a general mechanism for the unequivocal commitment underlying stem cell differentiation.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380013247338258178","@type":"Researcher","foaf:name":[{"@value":"Mati Mann"}],"jpcoar:affiliationName":[{"@value":"Departments of aMolecular Genetics and"},{"@value":"Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013247338258179","@type":"Researcher","foaf:name":[{"@value":"Omer Barad"}],"jpcoar:affiliationName":[{"@value":"Departments of aMolecular Genetics and"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013247338258176","@type":"Researcher","foaf:name":[{"@value":"Reuven Agami"}],"jpcoar:affiliationName":[{"@value":"Gene Regulation Division, Netherlands Cancer Institute, 1066 CX, Amsterdam, The Netherlands"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013247338258048","@type":"Researcher","foaf:name":[{"@value":"Benjamin Geiger"}],"jpcoar:affiliationName":[{"@value":"Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel; and"}]},{"@id":"https://cir.nii.ac.jp/crid/1380013247338258177","@type":"Researcher","foaf:name":[{"@value":"Eran Hornstein"}],"jpcoar:affiliationName":[{"@value":"Departments of aMolecular Genetics and"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"2010-08-18","prism:volume":"107","prism:number":"36","prism:startingPage":"15804","prism:endingPage":"15809"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.0915022107"}],"createdAt":"2010-08-19","modifiedAt":"2022-06-07","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360009142655185280","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"The miR‐155‐5p inhibits osteoclast differentiation through targeting CXCR2 in orthodontic root resorption"}]},{"@id":"https://cir.nii.ac.jp/crid/1360565168296641664","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Expression profiling of micro<scp>RNA</scp>s in <scp>RAW</scp>264.7 cells treated with a combination of tumor necrosis factor alpha and <scp>RANKL</scp> during osteoclast differentiation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360567189451898624","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"MicroRNAs and Osteolytic Bone Metastasis: The Roles of MicroRNAs in Tumor-Induced Osteoclast Differentiation"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848664423318400","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"MicroRNAs: Potential Biomarkers and Therapeutic Targets for Alveolar Bone Loss in Periodontal Disease"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1073/pnas.0915022107"},{"@type":"CROSSREF","@value":"10.1111/jre.12875_references_DOI_C6Fy9SadjSOK4q9yDiYV8OI27AM"},{"@type":"CROSSREF","@value":"10.1111/jre.12017_references_DOI_C6Fy9SadjSOK4q9yDiYV8OI27AM"},{"@type":"CROSSREF","@value":"10.3390/jcm4091741_references_DOI_C6Fy9SadjSOK4q9yDiYV8OI27AM"},{"@type":"CROSSREF","@value":"10.3390/ijms17081317_references_DOI_C6Fy9SadjSOK4q9yDiYV8OI27AM"}]}