{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670320964924288.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1038/s41467-020-16032-y"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41467-020-16032-y.pdf"}},{"identifier":{"@type":"URI","@value":"https://www.nature.com/articles/s41467-020-16032-y"}}],"dc:title":[{"@value":"Molecular mechanism of light-driven sodium pumping"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title>\n                  <jats:p>\n                    The light-driven sodium-pumping rhodopsin KR2 from\n                    <jats:italic>Krokinobacter eikastus</jats:italic>\n                    is the only non-proton cation active transporter with demonstrated potential for optogenetics. However, the existing structural data on KR2 correspond exclusively to its ground state, and show no sodium inside the protein, which hampers the understanding of sodium-pumping mechanism. Here we present crystal structure of the O-intermediate of the physiologically relevant pentameric form of KR2 at the resolution of 2.1 Å, revealing a sodium ion near the retinal Schiff base, coordinated by N112 and D116 of the characteristic NDQ triad. We also obtained crystal structures of D116N and H30A variants, conducted metadynamics simulations and measured pumping activities of putative pathway mutants to demonstrate that sodium release likely proceeds alongside Q78 towards the structural sodium ion bound between KR2 protomers. Our findings highlight the importance of pentameric assembly for sodium pump function, and may be used for rational engineering of enhanced optogenetic tools.\n                  </jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670320964924420","@type":"Researcher","foaf:name":[{"@value":"Kirill Kovalev"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924160","@type":"Researcher","foaf:name":[{"@value":"Roman Astashkin"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924425","@type":"Researcher","foaf:name":[{"@value":"Ivan Gushchin"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924297","@type":"Researcher","foaf:name":[{"@value":"Philipp Orekhov"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924293","@type":"Researcher","foaf:name":[{"@value":"Dmytro Volkov"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924289","@type":"Researcher","foaf:name":[{"@value":"Egor Zinovev"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924419","@type":"Researcher","foaf:name":[{"@value":"Egor Marin"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924300","@type":"Researcher","foaf:name":[{"@value":"Maksim Rulev"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924294","@type":"Researcher","foaf:name":[{"@value":"Alexey Alekseev"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924292","@type":"Researcher","foaf:name":[{"@value":"Antoine Royant"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924416","@type":"Researcher","foaf:name":[{"@value":"Philippe Carpentier"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924418","@type":"Researcher","foaf:name":[{"@value":"Svetlana Vaganova"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924290","@type":"Researcher","foaf:name":[{"@value":"Dmitrii Zabelskii"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924298","@type":"Researcher","foaf:name":[{"@value":"Christian Baeken"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924417","@type":"Researcher","foaf:name":[{"@value":"Ilya Sergeev"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924423","@type":"Researcher","foaf:name":[{"@value":"Taras Balandin"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924299","@type":"Researcher","foaf:name":[{"@value":"Gleb Bourenkov"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924296","@type":"Researcher","foaf:name":[{"@value":"Xavier Carpena"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924422","@type":"Researcher","foaf:name":[{"@value":"Roeland Boer"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924421","@type":"Researcher","foaf:name":[{"@value":"Nina Maliar"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924288","@type":"Researcher","foaf:name":[{"@value":"Valentin Borshchevskiy"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924295","@type":"Researcher","foaf:name":[{"@value":"Georg Büldt"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924291","@type":"Researcher","foaf:name":[{"@value":"Ernst Bamberg"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670320964924424","@type":"Researcher","foaf:name":[{"@value":"Valentin Gordeliy"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"20411723"}],"prism:publicationName":[{"@value":"Nature Communications"}],"dc:publisher":[{"@value":"Springer Science and Business Media LLC"}],"prism:publicationDate":"2020-05-01","prism:volume":"11","prism:number":"1","prism:startingPage":"2137"},"reviewed":"false","dc:rights":["https://creativecommons.org/licenses/by/4.0","https://creativecommons.org/licenses/by/4.0"],"url":[{"@id":"https://www.nature.com/articles/s41467-020-16032-y.pdf"},{"@id":"https://www.nature.com/articles/s41467-020-16032-y"}],"createdAt":"2020-05-01","modifiedAt":"2022-12-05","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050291158071078912","@type":"Article","resourceType":"学術雑誌論文(journal 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