{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670321305747712.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1002/yea.1023"}},{"identifier":{"@type":"URI","@value":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fyea.1023"}},{"identifier":{"@type":"URI","@value":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/yea.1023"}}],"dc:title":[{"@value":"Regulation of <scp>D</scp>‐amino acid oxidase expression in the yeast <i>Rhodotorula gracilis</i>"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:title>Abstract</jats:title><jats:p><jats:italic>Rhodotorula gracilis</jats:italic> is a oleaginous yeast which utilizes <jats:sc>D</jats:sc>‐amino acids as a source of carbon and/or nitrogen. <jats:sc>D</jats:sc>‐amino acid oxidase (DAAO), which converts <jats:sc>D</jats:sc>‐amino acids in the corresponding α‐keto acids and ammonia, is the first enzyme involved in the catabolism of <jats:sc>D</jats:sc>‐amino acids. DAAO activity is induced by the presence of <jats:sc>D</jats:sc>‐alanine, but the presence of the <jats:sc>L</jats:sc>‐isomer prevents induction by inhibiting the transport of <jats:sc>D</jats:sc>‐alanine into cells. To understand how DAAO expression is regulated, <jats:italic>R. gracilis</jats:italic> cells were grown on media containing different nitrogen and/or carbon sources. As a general rule, the level of DAAO mRNA reached a maximum after 15 h growth and preceded by ∼6 h the maximum level of DAAO activity. The inducer <jats:sc>D</jats:sc>‐alanine acts by increasing the rate of DAAO mRNA transcription: the increase in DAAO expression is due essentially to <jats:italic>de novo</jats:italic> synthesis. The presence of a supplemental carbon source (e.g. succinate or glucose) does not repress DAAO expression. Ammonium sulphate appears to have a negative effect on DAAO mRNA translation and on the expression of DAAO activity: DAAO is only partially active when the yeast is grown in the presence of <jats:sc>D</jats:sc>‐alanine and ammonium sulphate. The best expression of DAAO activity was obtained by growing the cells for 12 h at 30 °C in the presence of glucose and <jats:sc>D</jats:sc>‐alanine using cells pre‐cultured for 10 h on glucose and <jats:sc>L</jats:sc>‐alanine (0.99 U/mg protein, corresponding to ∼1.0% total proteins in the crude extract). Under these growth conditions a six‐fold increase in DAAO production was achieved. Copyright © 2003 John Wiley & Sons, Ltd.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1383670321305747715","@type":"Researcher","foaf:name":[{"@value":"Gianluca Molla"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670321305747712","@type":"Researcher","foaf:name":[{"@value":"Laura Motteran"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670321305747714","@type":"Researcher","foaf:name":[{"@value":"Luciano Piubelli"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670321305747713","@type":"Researcher","foaf:name":[{"@value":"Mirella S. Pilone"}]},{"@id":"https://cir.nii.ac.jp/crid/1383670321305747716","@type":"Researcher","foaf:name":[{"@value":"Loredano Pollegioni"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"0749503X"},{"@type":"EISSN","@value":"10970061"}],"prism:publicationName":[{"@value":"Yeast"}],"dc:publisher":[{"@value":"Wiley"}],"prism:publicationDate":"2003-08-05","prism:volume":"20","prism:number":"12","prism:startingPage":"1061","prism:endingPage":"1069"},"reviewed":"false","dc:rights":["http://onlinelibrary.wiley.com/termsAndConditions#vor"],"url":[{"@id":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fyea.1023"},{"@id":"https://onlinelibrary.wiley.com/doi/pdf/10.1002/yea.1023"}],"createdAt":"2003-09-03","modifiedAt":"2023-10-07","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360567181156263168","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"d-Amino Acid-Induced Expression of d-Amino Acid Oxidase in the Yeast Schizosaccharomyces pombe"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848656489876352","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"D-Amino acid oxidase of Streptomyces coelicolor and the effect of D-amino acids on the bacterium"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1002/yea.1023"},{"@type":"CROSSREF","@value":"10.1007/s00284-012-0227-z_references_DOI_9qvyHOLQYFkcQcR4OTARlrVW1Y"},{"@type":"CROSSREF","@value":"10.1007/s13213-013-0756-0_references_DOI_9qvyHOLQYFkcQcR4OTARlrVW1Y"}]}