{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1363670321339910400.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1073/pnas.96.23.13062"}},{"identifier":{"@type":"URI","@value":"https://pnas.org/doi/pdf/10.1073/pnas.96.23.13062"}}],"dc:title":[{"@value":"Geranyl diphosphate synthase: Cloning, expression, and characterization of this prenyltransferase as a heterodimer"}],"description":[{"type":"abstract","notation":[{"@value":"<jats:p>Geranyl diphosphate synthase, which catalyzes the condensation of dimethylallyl diphosphate and isopentenyl diphosphate to geranyl diphosphate, the key precursor of monoterpene biosynthesis, was purified from isolated oil glands of spearmint. Peptide fragments generated from the pure proteins of 28 and 37 kDa revealed amino acid sequences that matched two cDNA clones obtained by random screening of a peppermint-oil gland cDNA library. The deduced sequences of both proteins showed some similarity to existing prenyltransferases, and both contained a plastid-targeting sequence. Expression of each cDNA individually yielded no detectable prenyltransferase activity; however, coexpression of the two together produced functional geranyl diphosphate synthase. Antibodies raised against each protein were used to demonstrate that both subunits were required to produce catalytically active native and recombinant enzymes, thus confirming that geranyl diphosphate synthase is a heterodimer.</jats:p>"}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1380576285506924929","@type":"Researcher","foaf:name":[{"@value":"Charles C. Burke"}],"jpcoar:affiliationName":[{"@value":"Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576285506924930","@type":"Researcher","foaf:name":[{"@value":"Mark R. Wildung"}],"jpcoar:affiliationName":[{"@value":"Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340"}]},{"@id":"https://cir.nii.ac.jp/crid/1380576285506924928","@type":"Researcher","foaf:name":[{"@value":"Rodney Croteau"}],"jpcoar:affiliationName":[{"@value":"Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"00278424"},{"@type":"EISSN","@value":"10916490"}],"prism:publicationName":[{"@value":"Proceedings of the National Academy of Sciences"}],"dc:publisher":[{"@value":"Proceedings of the National Academy of Sciences"}],"prism:publicationDate":"1999-11-09","prism:volume":"96","prism:number":"23","prism:startingPage":"13062","prism:endingPage":"13067"},"reviewed":"false","url":[{"@id":"https://pnas.org/doi/pdf/10.1073/pnas.96.23.13062"}],"createdAt":"2002-07-26","modifiedAt":"2022-06-07","relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1050585658873035008","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Structure–function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204163424128","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Geranylgeranyl Diphosphate Synthase from Scoparia dulcis and Croton sublyratus. Plastid Localization and Conversion to a Farnesyl Diphosphate Synthase by Mutagenesis."}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204326606208","@type":"Article","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Recent progress in secondary metabolism of plant glandular trichomes"}]}],"dataSourceIdentifier":[{"@type":"CROSSREF","@value":"10.1073/pnas.96.23.13062"},{"@type":"CROSSREF","@value":"10.5511/plantbiotechnology.14.0701a_references_DOI_IMcnguZcfN6xzL0sBZM8Dr0qS0c"},{"@type":"CROSSREF","@value":"10.1248/cpb.49.197_references_DOI_IMcnguZcfN6xzL0sBZM8Dr0qS0c"},{"@type":"CROSSREF","@value":"10.1038/s42003-021-01739-5_references_DOI_IMcnguZcfN6xzL0sBZM8Dr0qS0c"}]}