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We show here that Nucling was up-regulated by proapoptotic stimuli and important for the induction of apoptosis after cytotoxic stress. We further demonstrated that overexpressed Nucling was able to induce apoptosis. In Nucling-deficient cells, the expression levels of Apaf-1 and cytochrome c, which are the major components of an apoptosis-promoting complex named apoptosome, were both down-regulated under cellular stress. A deficiency of Nucling also conferred resistance to apoptotic stress on the cell. After UV irradiation, Nucling was shown to reside in an Apaf-1/pro-caspase-9 complex, suggesting that Nucling might be a key molecule for the formation and maintenance of this complex. Nucling induced translocation of Apaf-1 to the nucleus, thereby distributing the Nucling/Apaf-1/pro-caspase-9 complex to the nuclear fraction. These findings suggest that Nucling recruits and transports the apoptosome complex during stress-induced apoptosis."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1384233268616555659","@type":"Researcher","foaf:name":[{"@value":"Takashi Sakai"}]},{"@id":"https://cir.nii.ac.jp/crid/1384233268616555650","@type":"Researcher","foaf:name":[{"@value":"Li Liu"}]},{"@id":"https://cir.nii.ac.jp/crid/1384233268616555651","@type":"Researcher","foaf:name":[{"@value":"Xichuan Teng"}]},{"@id":"https://cir.nii.ac.jp/crid/1384233268616555653","@type":"Researcher","foaf:name":[{"@value":"Rika Mukai-Sakai"}]},{"@id":"https://cir.nii.ac.jp/crid/1384233268616555648","@type":"Researcher","foaf:name":[{"@value":"Hidenori 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Vectors"},{"@id":"https://cir.nii.ac.jp/all?q=Down-Regulation","dc:title":"Down-Regulation"},{"@id":"https://cir.nii.ac.jp/all?q=Apoptosis","dc:title":"Apoptosis"},{"@id":"https://cir.nii.ac.jp/all?q=Mice,%20Transgenic","dc:title":"Mice, Transgenic"},{"@id":"https://cir.nii.ac.jp/all?q=Cysteine%20Proteinase%20Inhibitors","dc:title":"Cysteine Proteinase Inhibitors"},{"@id":"https://cir.nii.ac.jp/all?q=Transfection","dc:title":"Transfection"},{"@id":"https://cir.nii.ac.jp/all?q=Amino%20Acid%20Chloromethyl%20Ketones","dc:title":"Amino Acid Chloromethyl Ketones"},{"@id":"https://cir.nii.ac.jp/all?q=Cell%20Line","dc:title":"Cell Line"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=In%20Situ%20Nick-End%20Labeling","dc:title":"In Situ Nick-End 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