{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1364233268647148672.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1093/mutage/geu062"}},{"identifier":{"@type":"URI","@value":"http://academic.oup.com/mutage/article-pdf/30/2/227/17163126/geu062.pdf"}},{"identifier":{"@type":"PMID","@value":"25392148"}}],"dc:title":[{"@value":"Acrylamide induces specific DNA adduct formation and gene mutations in a carcinogenic target site, the mouse lung"}],"description":[{"notation":[{"@value":"Acrylamide (AA) is a contaminant in heated foods and is carcinogenic in multiple organs of rodents. There have been many reports regarding AA-induced DNA modification and genotoxicity. However, the data are insufficient to understand fully the relationship between the two events. A recent report demonstrated carcinogenicity in the mouse lung. The lung is advantageous for investigation of AA-induced genotoxicity because DNA adduct levels are relatively high in this organ. In the present study, reporter gene mutation assays and quantitative analyses of specific DNA adducts were performed in the lungs of mature gpt delta mice treated with AA at doses of 100, 200 and 400 p.p.m. in drinking water for 4 weeks. N7-GA-Gua was detected in all AA-treated mice in a dose-dependent manner. gpt mutant frequencies (MFs) were significantly increased in the middle- and high-dose groups. In the analysis of mutation spectra, significant increases in GC-TA transversions and single base deletion mutations were observed in the high-dose group. Spi(-) MFs were significantly increased in the high-dose group. Analysis of Spi(-) mutants revealed significant increases in the frequencies of single base deletion mutation in runs of G/C and A/T. Analyses of immature mice under the same experimental conditions showed that there were no differences of susceptibility to AA-induced genotoxicity in the two age classes. The overall data clearly show the causal relationship between AA-induced DNA adducts and the gene mutations at carcinogenic target sites."}]}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1384233268647148676","@type":"Researcher","foaf:name":[{"@value":"Yuji Ishii"}]},{"@id":"https://cir.nii.ac.jp/crid/1384233268647148673","@type":"Researcher","foaf:name":[{"@value":"Kohei Matsushita"}]},{"@id":"https://cir.nii.ac.jp/crid/1384233268647148545","@type":"Researcher","foaf:name":[{"@value":"Ken Kuroda"}]},{"@id":"https://cir.nii.ac.jp/crid/1384233268647148547","@type":"Researcher","foaf:name":[{"@value":"Yuh Yokoo"}]},{"@id":"https://cir.nii.ac.jp/crid/1384233268647148675","@type":"Researcher","foaf:name":[{"@value":"Aki Kijima"}]},{"@id":"https://cir.nii.ac.jp/crid/1420845751135437952","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00597891"},{"@type":"NRID","@value":"1000000597891"},{"@type":"NRID","@value":"9000317128298"},{"@type":"NRID","@value":"9000018986317"},{"@type":"NRID","@value":"9000386308177"},{"@type":"NRID","@value":"9000397862491"},{"@type":"NRID","@value":"9000283730394"},{"@type":"NRID","@value":"9000345266629"},{"@type":"NRID","@value":"9000396134189"},{"@type":"NRID","@value":"9000403275105"},{"@type":"NRID","@value":"9000397862775"},{"@type":"NRID","@value":"9000317127584"},{"@type":"NRID","@value":"9000365518379"},{"@type":"NRID","@value":"9000345267484"},{"@type":"NRID","@value":"9000257820905"},{"@type":"NRID","@value":"9000347140704"},{"@type":"NRID","@value":"9000258714183"},{"@type":"NRID","@value":"9000010567597"},{"@type":"NRID","@value":"9000347192452"},{"@type":"NRID","@value":"9000240043066"},{"@type":"NRID","@value":"9000410150720"},{"@type":"NRID","@value":"9000317127578"},{"@type":"NRID","@value":"9000411486837"},{"@type":"NRID","@value":"9000317127921"},{"@type":"NRID","@value":"9000283730969"},{"@type":"NRID","@value":"9000258714401"},{"@type":"NRID","@value":"9000317127915"},{"@type":"NRID","@value":"9000240184360"},{"@type":"NRID","@value":"9000347138658"},{"@type":"NRID","@value":"9000001951686"},{"@type":"NRID","@value":"9000410150374"},{"@type":"NRID","@value":"9000317127490"},{"@type":"NRID","@value":"9000283729916"},{"@type":"NRID","@value":"9000001981045"},{"@type":"NRID","@value":"9000365515278"},{"@type":"NRID","@value":"9000242883281"},{"@type":"NRID","@value":"9000386308524"},{"@type":"NRID","@value":"9000347192522"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/reschmst"}],"foaf:name":[{"@value":"Shinji Takasu"}]},{"@id":"https://cir.nii.ac.jp/crid/1384233268647148672","@type":"Researcher","foaf:name":[{"@value":"Yukio Kodama"}]},{"@id":"https://cir.nii.ac.jp/crid/1384233268647148544","@type":"Researcher","foaf:name":[{"@value":"Akiyoshi Nishikawa"}]},{"@id":"https://cir.nii.ac.jp/crid/1384233268647148674","@type":"Researcher","foaf:name":[{"@value":"Takashi Umemura"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"14643804"},{"@type":"PISSN","@value":"02678357"}],"prism:publicationName":[{"@value":"Mutagenesis"}],"dc:publisher":[{"@value":"Oxford University Press (OUP)"}],"prism:publicationDate":"2014-11-12","prism:volume":"30","prism:number":"2","prism:startingPage":"227","prism:endingPage":"235"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://academic.oup.com/mutage/article-pdf/30/2/227/17163126/geu062.pdf"}],"createdAt":"2014-11-13","modifiedAt":"2022-04-22","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Acrylamide","dc:title":"Acrylamide"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Mutational%20Analysis","dc:title":"DNA Mutational Analysis"},{"@id":"https://cir.nii.ac.jp/all?q=DNA%20Adducts","dc:title":"DNA Adducts"},{"@id":"https://cir.nii.ac.jp/all?q=Mice","dc:title":"Mice"},{"@id":"https://cir.nii.ac.jp/all?q=Mutation","dc:title":"Mutation"},{"@id":"https://cir.nii.ac.jp/all?q=Carcinogens","dc:title":"Carcinogens"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Lung","dc:title":"Lung"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360285707357259264","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Evaluation of mutagenicity of acrylamide using RBC Pig-a and PIGRET assays by single peroral dose in rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1360286994458201216","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"An organoid-based carcinogenesis model induced by in vitro chemical 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