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Sodium dodecyl sulfate gel electrophoresis showed that the molecular weight of the main band was about 230kDa. Although the molecular weight was quite similar to that of the heavy chain of muscle myosin (myosin II), the motor protein was soluble at low ionic strength and antibody raised against the main band did not recognize smooth muscle myosin. The antibody also did not recognize the actin based motor protein from lily pollen tube. The motor protein translocated fluorescently labeled actin filaments at about 25μm/s in the <i>in vitro</i> motility assay. The MgATPase activity of the motor protein was enhanced by F-actin about 150 fold. Calcium ion concentration had little effect on both the motor activity and the actin activated MgATPase activity."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410001204145615747","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020266302"}],"foaf:name":[{"@language":"en","@value":"YAMAMOTO Keiichi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Bioengineering, Faculty of Engineering, Soka University"}]},{"@id":"https://cir.nii.ac.jp/crid/1030285133407596673","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"90131010"},{"@type":"NRID","@value":"1000090131010"},{"@type":"NRID","@value":"9000000082697"},{"@type":"NRID","@value":"9000002737193"},{"@type":"NRID","@value":"9000005476149"},{"@type":"NRID","@value":"9000020509965"},{"@type":"NRID","@value":"9000391949462"},{"@type":"NRID","@value":"9000391927203"},{"@type":"NRID","@value":"9000242131853"},{"@type":"NRID","@value":"9000015686176"},{"@type":"NRID","@value":"9000391942751"},{"@type":"NRID","@value":"9000391946774"},{"@type":"NRID","@value":"9000391928648"},{"@type":"NRID","@value":"9000391935702"},{"@type":"NRID","@value":"9000001678627"},{"@type":"NRID","@value":"9000001427262"},{"@type":"NRID","@value":"9000006970786"},{"@type":"NRID","@value":"9000391946778"},{"@type":"NRID","@value":"9000007168806"},{"@type":"NRID","@value":"9000020266312"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0019672"}],"foaf:name":[{"@language":"en","@value":"KIKUYAMA Munehiro"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Biological Laboratory, Faculty of Liberal Arts, The University of the Air"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001204145615744","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000020266327"}],"foaf:name":[{"@language":"en","@value":"SUTOH-YAMAMOTO Noriko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Bioengineering, Faculty of Engineering, Soka University"}]},{"@id":"https://cir.nii.ac.jp/crid/1030566608940299779","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60111697"},{"@type":"NRID","@value":"1000060111697"},{"@type":"NRID","@value":"9000020698780"},{"@type":"NRID","@value":"9000005475639"},{"@type":"NRID","@value":"9000002456664"},{"@type":"NRID","@value":"9000253244026"},{"@type":"NRID","@value":"9000007303658"},{"@type":"NRID","@value":"9000256212103"},{"@type":"NRID","@value":"9000017625418"},{"@type":"NRID","@value":"9000253244065"},{"@type":"NRID","@value":"9000020683117"},{"@type":"NRID","@value":"9000017800167"},{"@type":"NRID","@value":"9000020266342"}],"foaf:name":[{"@language":"en","@value":"KAMITSUBO Eiji"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Biological Laboratory, Hitotsubashi University"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03862208"},{"@type":"EISSN","@value":"13492896"}],"prism:publicationName":[{"@language":"en","@value":"Proceedings of the Japan Academy, Series B"},{"@language":"ja","@value":"Ｐｒｏｃｅｅｄｉｎｇｓ　ｏｆ　ｔｈｅ　Ｊａｐａｎ　Ａｃａｄｅｍｙ．　Ｓｅｒ．　Ｂ：　Ｐｈｙｓｉｃａｌ　ａｎｄ　Ｂｉｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ"},{"@language":"en","@value":"Proc. Jpn. Acad., Ser. B"},{"@language":"en","@value":"Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences"},{"@language":"ja","@value":"Ｐｒｏｃｅｅｄｉｎｇｓ　ｏｆ　ｔｈｅ　Ｊａｐａｎ　Ａｃａｄｅｍｙ．　Ｓｅｒ．　Ｂ：　Ｐｈｙｓｉｃａｌ　ａｎｄ　Ｂｉｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ"}],"dc:publisher":[{"@language":"en","@value":"The Japan Academy"},{"@language":"ja","@value":"日本学士院"}],"prism:publicationDate":"1994","prism:volume":"70","prism:number":"10","prism:startingPage":"175","prism:endingPage":"180"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"http://www.jstage.jst.go.jp/article/pjab1977/70/10/70_10_175/_pdf"}],"availableAt":"1994","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Cytoplasmic%20streaming","dc:title":"Cytoplasmic streaming"},{"@id":"https://cir.nii.ac.jp/all?q=%3Ci%3EChara%20corallina%3C/i%3E","dc:title":"<i>Chara corallina</i>"},{"@id":"https://cir.nii.ac.jp/all?q=motor%20protein","dc:title":"motor protein"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002215888391808","@type":"Article","resourceType":"学術雑誌論文(journal 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