Measurement of triiodothyronine in urine.

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  • YOSHIDA KATSUMI
    The Second Department of Internal Medicine, Tohoku University School of Medicine
  • SAKURADA TOSHIRO
    The Second Department of Internal Medicine, Tohoku University School of Medicine
  • KAISE NOBUKO
    The Second Department of Internal Medicine, Tohoku University School of Medicine
  • YAMAMOTO MAKIKO
    The Second Department of Internal Medicine, Tohoku University School of Medicine
  • KAISE KAZUO
    The Second Department of Internal Medicine, Tohoku University School of Medicine
  • SAITO SHINTARO
    The Second Department of Internal Medicine, Tohoku University School of Medicine
  • YOSHINAGA KAORU
    The Second Department of Internal Medicine, Tohoku University School of Medicine

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タイトル別名
  • Measurement of Triiodothyronine in Urin

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A radioimmunoassay procedure to measure triiodothyronine (T3) in unextracted urine is described. One hundred, μl of T3 standards or samples were incubated with 100, μl of T3 antiserum (1:40, 000), 100μl of tracer 125I-T3 and 700μl of 0.05M borate buffer, pH 8.6. Separation of free from bound antigen was achieved by dextran coated charcoal. The cross-reaction of L-T3 with L-T4 in this assay system was less than 0.2%. Dilution of high T3 urine was parallel to the standard curve. Recovery was 101±8%. Coefficients of variation were 3-8% within each assay and 13% between assays. Euthyroid subjects excreted 0.81±0.39μg (mean±S. D. ) in 24hr. Similar values were obtained in pregnant women, euthyroid patients with chronic thyroiditis, and a patient with thyroxine-binding globulin deficiency. Excretion of urinary T3 was high (7.48±3.32μg/24h) in patients with hyperthyroidism, and low (0.14±0.15μg/24hr) in patients with hypothyroidism. Urinary T3 excretion was almost undetectable in terminal renal failure. Positive correlations were found between urinary T3 and serum total T3 (r=0.89) and especially serum free T3 (r=0.97). A positive correlation was also found between urinary T3 and creatinine clearance (r=0.63). Mean urinary clearance of T3(CT3=Urinary T3 excretion/serum free T3 was 166ml/min and was significantly higher than their mean creatinine clearance (110ml/min). The measurement of T3 in urine is reliable and easy to perform, and may allow a new approach to the understanding of thyroid hormone metabolism.

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