Glucose Oxidase Immobilized in Alginate/Layered Double Hydroxides Hybrid Membrane and Its Biosensing Application

DOI PDF Web Site Web Site Web Site 82 References
  • DING Shou-Nian
    School of Chemistry & Chemical Engineering, Southeast University
  • SHAN Dan
    School of Chemistry & Chemical Engineering, Yangzhou University
  • XUE Huai-Guo
    School of Chemistry & Chemical Engineering, Yangzhou University
  • ZHU Dao-Bin
    School of Chemistry & Chemical Engineering, Yangzhou University
  • COSNIER Serge
    Départment de Chimie Moléculaire UMR 5250, ICMG FR 2607, CNRS Université Joseph Fourier

Bibliographic Information

Published
2009
DOI
  • 10.2116/analsci.25.1421
Publisher
The Japan Society for Analytical Chemistry

Search this article

Description

A new type of amperometric glucose biosensor based on alginate (Alg)/layered double hydroxides (LDHs) organic-inorganic composite film is described. This hybrid material combines the advantages of an organic biopolymer, Alg and inorganic LDHs. Glucose oxidase (GOD) immobilized in the material maintained its activity. The composite films were characterized by UV-Vis. The results indicated that GOD retained the essential feature of its native structure in the composite film. The Alg/LDHs/GOD-modified platinum electrode exhibited a fast response to glucose (achieve 95% of the maximum in 10 s), which provided a linear response to glucose over a concentration range of 1.6 × 10−5 − 2 × 10−3 M with a detection limit of 4 × 10−5 M based on S/N = 3. Furthermore, the biosensor exhibited excellent long-term stability, and satisfactory reproducibility. It retained 87% of its original activity after being used for 28 days.

Journal

  • Analytical Sciences

    Analytical Sciences 25 (12), 1421-1425, 2009

    The Japan Society for Analytical Chemistry

References(82)*help

See more

Report a problem

Back to top