Evaluation of an ODS Column Modified with Zwitterionic/Nonionic Mixed Surfactants and Its Application to Direct Injection Determination of Inorganic Anions
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- HASEGAWA Takuya
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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- UMEMURA Tomonari
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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- KOIDE Akira
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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- CHIBA Koichi
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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- UEKI Yuji
- Department of Chemistry, Faculty of Engineering, Gunma University
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- TSUNODA Kin-ichi
- Department of Chemistry, Faculty of Engineering, Gunma University
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- HARAGUCHI Hiroki
- Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
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説明
An octadecylsilica (ODS) column modified with zwitterionic/nonionic mixed surfactants was evaluated for the direct injection determination of inorganic anions in biological fluids by ion chromatography. A zwitterionic surfactant (sulfobetaine-type) and a nonionic surfactant (polyoxyethylene-type) were used for a stationary-phase modification. When aqueous electrolyte solutions with concentrations of sub-mM to several mM were used as a mobile phase, the zwitterionic surfactant coated on the ODS surface exhibited unique separation selectivity for ionic species, while the nonionic surfactant coated on the ODS might have formed a hydrophilic network over the ODS surface and restricted matrix proteins from adsorbing on the stationary phase. Consequently, the mixed surfactant-modified column system allowed an efficient ion chromatographic separation of inorganic anions as well as a size-exclusive removal of column-fouling proteins. This separation system was applied to the direct injection determination of UV-absorbing anions in human saliva. The detection limits for nitrite, nitrate, iodide and thiocyanate were 3.1, 2.7, 4.5 and 25 µM, respectively, with UV detection at 210 nm (injection volume; 20 µl), and their relative standard deviations for 5 replicate measurements of saliva samples spiked with 100 µM each of those anions were 1.4, 0.9, 2.2 and 5.5%, respectively.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 21 (8), 913-916, 2005
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204258494848
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- NII論文ID
- 130004441116
- 10016644477
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD2MXoslGgt7k%3D
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- ISSN
- 13482246
- 09106340
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- HANDLE
- 2237/8754
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- NDL書誌ID
- 7383904
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- PubMed
- 16122160
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- Web Site
- https://nagoya.repo.nii.ac.jp/records/7080
- http://id.ndl.go.jp/bib/7383904
- https://ndlsearch.ndl.go.jp/books/R000000004-I7383904
- https://link.springer.com/content/pdf/10.2116/analsci.21.913.pdf
- https://link.springer.com/article/10.2116/analsci.21.913/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/21/8/21_8_913/_pdf
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- 本文言語コード
- en
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