Purification of Pyridylaminated Oligosaccharides Using 1,2-Dichloroethane Extraction
-
- SUZUKI Yusuke
- College of Science and Technology, Nihon University
-
- OKANO Aya
- College of Science and Technology, Nihon University
-
- KABAYAMA Kazuya
- Department of Chemistry, Graduate School of Science, Osaka University
-
- NISHINA Atsuyoshi
- College of Science and Technology, Nihon University
-
- TANIGAWA Minoru
- College of Science and Technology, Nihon University
-
- NISHIMURA Katsushi
- College of Science and Technology, Nihon University
-
- KUSHI Yasunori
- College of Science and Technology, Nihon University
この論文をさがす
説明
Fluorescence derivatization of the oligosaccharides released from glycoconjugates is widely used for precise structural characterization. To ensure labeling of the oligosaccharides, a large excess of fluorescence reagents is usually added to the reaction tube. Therefore, any excess reagents and by-products of the labeling reaction should be removed by several column chromatographies, including using a cellulose cartridge or spin columns. However, these purification steps are often time-consuming, expensive, and laborious. In this study, we found that 1,2-dichloroethane extraction could effectively and easily purify pyridylaminated oligosaccharides with a high recovery rate.
収録刊行物
-
- Analytical Sciences
-
Analytical Sciences 32 (5), 487-490, 2016
社団法人 日本分析化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001204260163456
-
- NII論文ID
- 130005150941
-
- NII書誌ID
- AA10500785
-
- ISSN
- 13482246
- 09106340
-
- NDL書誌ID
- 027283510
-
- PubMed
- 27169645
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可