Use of a Holder-vacuum Tube Device to Save On-site Hands in Preparing Urine Samples for Head-space Gas-chromatography, and Its Application to Determine the Time Allowance for Sample Sealing
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- KAWAI Toshio
- Osaka Occupational Health Service Center, Japan Industrial Health Safety and Health Association
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- SUMINO Kimiaki
- Osaka Occupational Health Service Center, Japan Industrial Health Safety and Health Association
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- OHASHI Fumiko
- Kyoto Industrial Health Association (Head office)
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- IKEDA Masayuki
- Kyoto Industrial Health Association (Head office)
Bibliographic Information
- Other Title
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- Use of a holder-vacuum tube to save on-site hands in preparing urine for head-space gas-chromatography, and its application to determine the time allowance for sample sealing
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Description
To facilitate urine sample preparation prior to head-space gas-chromatographic (HS-GC) analysis. Urine samples containing one of the five solvents (acetone, methanol, methyl ethyl ketone, methyl isobutyl ketone and toluene) at the levels of biological exposure limits were aspirated into a vacuum tube via holder, a device commercially available for venous blood collection (the vacuum tube method). The urine sample, 5 ml, was quantitatively transferred to a 20-ml head-space vial prior to HS-GC analysis. The loaded tubes were stored at +4℃ in dark for up to 3 d. The vacuum tube method facilitated on-site procedures of urine sample preparation for HS-GC with no significant loss of solvents in the sample and no need of skilled hands, whereas on-site sample preparation time was significantly reduced. Furthermore, no loss of solvents was detected during the 3-d storage, irrespective of hydrophilic (acetone) or lipophilic solvent (toluene). In a pilot application, high performance of the vacuum tube method in sealing a sample in an air-tight space succeeded to confirm that no solvent will be lost when sealing is completed within 5 min after urine voiding, and that the allowance time is as long as 30 min in case of toluene in urine. The use of the holder-vacuum tube device not only saves hands for transfer of the sample to air-tight space, but facilitates sample storage prior to HS-GC analysis.<br>
Journal
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- Industrial Health
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Industrial Health 49 (1), 24-29, 2011
National Institute of Occupational Safety and Health
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Details 詳細情報について
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- CRID
- 1390001204280270976
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- NII Article ID
- 130004828589
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- NII Book ID
- AA00672955
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- ISSN
- 18808026
- 00198366
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- NDL BIB ID
- 10972135
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed