Application of Ribosomal Intergenic Spacer Analysis to the Characterization of Microbial Communities in Rhizospheric Soils of the Bt Transgenic Chrysanthemum

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  • Ikeda Seishi
    Gene Research Center, University of Tsukuba
  • Shinoyama Harue
    Fukui Agricultural Experiment Station
  • Yamanaka Shinsuke
    Plant Genetic Resources Laboratory, Genebank, National Institute of Agrobiological Sciences
  • Ytow Nozomi
    Gene Research Center, University of Tsukuba Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Ezura Hiroshi
    Gene Research Center, University of Tsukuba Graduate School of Life and Environmental Sciences, University of Tsukuba
  • Fujimura Tatsuhito
    Gene Research Center, University of Tsukuba Graduate School of Life and Environmental Sciences, University of Tsukuba

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Description

In the present study, we examined the feasibility of using a simplified ribosomal intergenic spacer analysis (RISA) technique to investigate the impact of chrysanthemum genotypes, including Bt transgenic lines, the control parental line and a wild species, on microbial communities in their rhizospheric soils. Differential bands among the plant genotypes were identified from RISA profiles. Sequence data revealed that some bands showed high levels of similarity to known species, such as a fungal group of Exidiopsis and Exidia and a protozoan, Tetrahymena tropicalis. None, however, shared homology to either pathogenic or beneficial microbes that have previously been identified in association with both animals and plants.<br>

Journal

  • Microbes and Environments

    Microbes and Environments 20 (4), 258-263, 2005

    Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

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