[Updated on Apr. 18] Integration of CiNii Articles into CiNii Research

68. Cyanamide-biosynthesizing organ in hairy vetch Vicia villosa subsp. varia

DOI

Bibliographic Information

Other Title
  • 68.ヘアリーベッチにおけるシアナミドの生合成部位(口頭発表)

Search this article

Abstract

Cyanamide (NH_2CN) has been isolated from hairy vetch Vicia villosa subsp. varia as a natural product for the first time. We have already clarified that the cyanamide content in hairy vetch seedlings cultivated in the growth chamber reaches its maximum at 3-4 week. The present study aims at elucidating the organ in which cyanamide is biosynthesized. The cyanamide contents in the leaves, the stems, and the roots of 4-week-old seedlings were 629 ± 209, 256 ± 134, and < 20 μg/gFW (average ± SD), respectively. This suggests that cyanamide is biosynthesized in the aerial parts (the leaves plus the stems). To investigate the cyanamide-biosynthesizing organ, we compared the ^^<15>N incorporation into cyanamide in the whole aerial parts to that in the apical portion including the younger leaves. The ^^<15>N isotope abundance ratio in the isolated cyanamide was 3.99 ± 0.61% (average ± SE) when the whole aerial parts was incubated with ^^<15>N-labeled nitrogen sources, which was significantly higher than that of control (0.28 ± 0.03%). In contrast, the cyanamide isolated from the apical portion was not ^^<15>N-labeled on the same condition. These results imply that cyanamide is biosynthesized in the mature leaves.

Journal

Details

  • CRID
    1390001204423356544
  • NII Article ID
    110006474761
  • NII Book ID
    AA11550064
  • DOI
    10.18978/jscrpanb.42.0_81
  • ISSN
    21896313
    09191887
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

Report a problem

Back to top