Production of somatic hybrid plant by means of a new electrofusion technique in a convergent electric field.

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  • 異種植物細胞間電気融合のための電界収束型電極を用いた基礎的研究
  • イシュ ショクブツ サイボウカン デンキ ユウゴウ ノ タメ ノ デンカイ シ

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New equipment for electrofusion of plant cells was developed for the purpose of automatic cell handling and fusion with electrical control of cell movement under a microscopic view, using the electrode configuration of a convergent electric field. Two cells (A and B) of different species introduced from separate inlet ports were moved toward the position of the convergent electric field by the applied 1MHz high frequency AC gradient field. The cells became attached to each other, making a pearl chain. When a pulsed electric field was applied to the electrodes, the two cells fused into one hybrid cell (A + B cell). The velocity of the dielectrophoretic cell movement and the operative conditions of the electrofusion were obtained using three kinds of plant cells to defermine the functions of applied AC voltage and frequency, pulse voltage and width. respectively.

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