Amplification and identification of cry II genes from Bacillus thuringiensis by PCR procedure.

DOI

Bibliographic Information

Other Title
  • ＰＣＲ法によるＢａｃｉｌｌｕｓ　ｔｈｕｒｉｎｇｉｅｎｓｉｓ　ｃｒｙ　ＩＩ遺伝子の増幅と同定

Description

Oligonucleotide primers including a specific domain of cry II gene sequences were synthesized in order to identify cry II genes which encode the expression of the P2 protein in Bacillus thuringiensis (Bt) strains. The cry II genes of the Bt isolates were amplified by polymerase chain reaction (PCR) and the amplified DNA was used as DNA probe for identification. Cry II B was distinguished from cry II A by Hinc II digestion. The cry II genes in the Bt isolates were identified by agarose gel electrophoresis. Based on the identification of the Bt subspecies, kurstaki HD-1, aizawai IPL and galleriae harboured both cry II A and cry II B genes, which kenyae harboured only the cry II A gene. This procedure was effective for the identification of Bt isolates including both lepidopteracidal and dipteracidal proteins.

Journal

Details 詳細情報について

  • CRID
    1390001204528749056
  • NII Article ID
    130003834789
  • DOI
    10.11416/kontyushigen1930.62.223
  • ISSN
    1884796X
    00372455
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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