Changes in esterase zymograms of the silkworm, <I>Bombyx mori</I> L., during development

DOI
  • EGUCHI Masaharu
    Faculty of Textile Fibers, Kyoto University of Industrial Arts and Textile Fibers
  • SUGIMOTO Tatsuyoshi
    Faculty of Textile Fibers, Kyoto University of Industrial Arts and Textile Fibers

Bibliographic Information

Other Title
  • 家蚕の発育に伴なうエステラーゼ型の変化

Description

By means of thin layer electrophoresis in agar gel, esterase of homogenates of various organs from the silkworm was studied.<BR>Fifteen different esterase bands were detected with β-naphthyl acetate as the substrate and naphthanil diazoblue B as coupler. Thirteen bands migrated toward the anode, while two bands migrated toward the cathodal side.<BR>There is a stage and tissue specificity in the patterns observed as in other organisms. Gradual changes in enzyme activity occurred during development, and there were great changes from the late period of the fifth instar to the pupal stage. In the alimentary canal and gonads, the stage specificity is based on the relative intensity or upon the presence or absence of a particular band. On the other hand, G, H and I bands in zymograms of the fat body and haemolymph showed yellowish brown color in the early period of development. Then, after the fifth instar these bands changed into red color as others. From above results it seems that esterase in G, H and I bands is synthesized during development.

Journal

Details 詳細情報について

  • CRID
    1390001204530722048
  • NII Article ID
    130004029747
  • DOI
    10.11416/kontyushigen1930.33.321
  • ISSN
    1884796X
    00372455
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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