Changes in esterase zymograms of the silkworm, <I>Bombyx mori</I> L., during development
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- EGUCHI Masaharu
- Faculty of Textile Fibers, Kyoto University of Industrial Arts and Textile Fibers
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- SUGIMOTO Tatsuyoshi
- Faculty of Textile Fibers, Kyoto University of Industrial Arts and Textile Fibers
Bibliographic Information
- Other Title
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- 家蚕の発育に伴なうエステラーゼ型の変化
Description
By means of thin layer electrophoresis in agar gel, esterase of homogenates of various organs from the silkworm was studied.<BR>Fifteen different esterase bands were detected with β-naphthyl acetate as the substrate and naphthanil diazoblue B as coupler. Thirteen bands migrated toward the anode, while two bands migrated toward the cathodal side.<BR>There is a stage and tissue specificity in the patterns observed as in other organisms. Gradual changes in enzyme activity occurred during development, and there were great changes from the late period of the fifth instar to the pupal stage. In the alimentary canal and gonads, the stage specificity is based on the relative intensity or upon the presence or absence of a particular band. On the other hand, G, H and I bands in zymograms of the fat body and haemolymph showed yellowish brown color in the early period of development. Then, after the fifth instar these bands changed into red color as others. From above results it seems that esterase in G, H and I bands is synthesized during development.
Journal
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- The Journal of Sericultural Science of Japan
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The Journal of Sericultural Science of Japan 33 (4), 321-326, 1964
The Japanese Society of Sericultural Science
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Details 詳細情報について
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- CRID
- 1390001204530722048
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- NII Article ID
- 130004029747
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- ISSN
- 1884796X
- 00372455
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed