Preventive Effect of 7,8-Dihydroxyflavone against Oxidative Stress Induced Genotoxicity
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- Zhang Rui
- School of Medicine and Applied Radiological Science Research Institute, Cheju National University
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- Kang Kyoung Ah
- School of Medicine and Applied Radiological Science Research Institute, Cheju National University
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- Piao Mei Jing
- School of Medicine and Applied Radiological Science Research Institute, Cheju National University
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- Ko Dong Ok
- School of Medicine and Applied Radiological Science Research Institute, Cheju National University
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- Wang Zhi Hong
- School of Medicine and Applied Radiological Science Research Institute, Cheju National University
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- Chang Weon Young
- School of Medicine and Applied Radiological Science Research Institute, Cheju National University
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- You Ho Jin
- Department of Pharmacology, College of Medicine, Chosun University
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- Lee In Kyung
- Department of Microbiology and Cancer Research Institute, Seoul National University College of Medicine
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- Kim Bum Joon
- Department of Microbiology and Cancer Research Institute, Seoul National University College of Medicine
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- Kang Sam Sik
- Natural Products Research Institute and College of Pharmacy, Seoul National University
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- Hyun Jin Won
- School of Medicine and Applied Radiological Science Research Institute, Cheju National University
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説明
We elucidated the protective effect of 7,8-dihydroxyflavone against hydrogen peroxide (H2O2)-induced DNA damage. We found that 7,8-dihydroxyflavone scavenges 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS). 7,8-Dihydroxyflavone with antioxidant effect prevented the H2O2-induced cellular DNA damage, as evidenced by comet tail, 8-hydroxy-2′-deoxyguanosine (8-OHdG) content, and phospho-histone H2A.X protein expression. Hence, 7,8-dihydroxyflavone was shown to protect cell via the inhibition of apoptosis induced by H2O2. This was substantiated by decreased apoptotic nuclear fragmentation, decreased sub-G1 cell population, and decreased DNA fragmentation. Furthermore, 7,8-dihydroxyflavone activated the protein kinase B (PKB, Akt) signal pathway, which is a major survival signal pathway. In addition, LY294002, which is phosphatidylinositol 3 kinase (PI3K, upstream of Akt) inhibitor, attenuated the protective effect of 7,8-dihydroxyflavone against H2O2-induced cell damage. In conclusion, 7,8-dihydroxyflavone was shown to possess cytoprotective properties against oxidative stress by scavenging intracellular ROS and enhancing Akt activity.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 32 (2), 166-171, 2009
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204628067456
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- NII論文ID
- 110007042167
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 9775023
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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