{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390001204766964352.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2530/jslsm.30.394"}},{"identifier":{"@type":"NAID","@value":"10026089039"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2010180377"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Preparation of Novel Organosilica Nanoparticles and Their Application for Photodynamic Therapy"},{"@language":"ja","@value":"新規な有機シリカナノ粒子の作製と光線力学的治療への応用"}],"dc:language":"ja","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Nanoparticles have been proposed as potential carriers for photosensitizers. Novel silica nanoparticles made of organosilica (Organo-NPs) have been successfully synthesized by using a St?ber method. We compared the Organo-NPs to classical silica nanoparticles made of tetraethoxysilane (TEOS NPs). The Organo-NPs were well dispersed in solution and have unique surface properties such as thiol residues on the surface and reduced zeta potential compared with TEOS NPs. The Organo-NPs with fluorescent dye distributed throughout the particle were synthesized by a method that deposited fluorescent dye on the silica network via a succinimidyl ester reaction with (3-aminopropyl) trimethoxysilane and via a maleimide reaction with organosilica. The resulting Organo-NPs with fluorescent were bright, non-aggregated in solution, and photostable. The fluorescence intensity and photostability of fluorescent Organo-NPs were sufficient for detection as a single fluorescent particle using flow cytometry and fluorescence microscopy. Protein-modified Organo-NPs were easily prepared by absorption and by maleimide coupling. The Organo-NPs did not exhibit death or cytotoxic findings on in vivo injection. The Organo-NPs containing photosensitizers showed phototoxic activity in culture cells. We demonstrate the usefulness of the Organo-NPs and their surface properties, and discuss their advantages for biological applications and possibility for photodynamic therapy."},{"@language":"ja","@value":"光線力学的治療に用いる光感受性物質をナノサイズのキャリアーに取り込ませナノ粒子化することにより様々な有用性が得られると考えられている．新規なナノ粒子である有機シリカナノ粒子を作製し，従来型シリカ粒子と比較すると共に光線力学的治療への応用を検討した．有機シリカナノ粒子は一段階の反応にて作製でき，サイズ制御，分散性も良好であった．粒子は作製した時点で表面ならび内部にチオール基などの官能基を有しており，内部機能化としてローダミンなどの蛍光色素が効率良く粒子内に取り込まれ蛍光有機シリカナノ粒子の作製も可能であった．表面機能化として粒子表面に抗体，アビジンなどのバイオ分子の吸着や化学修飾剤を用いた結合が容易であり，粒子の多機能化において高い発展性が認められた．また蛍光有機ナノシリカ粒子をマウスへ経静脈投与または腹腔内投与を行なったが明らかな毒性は認められなかった．さらに光感受性物質を含有した有機シリカナノ粒子を取り込んだ培養細胞に励起光を照射することにより細胞の形態変化が確認でき，ナノ粒子による細胞障害活性を示唆する所見が得られた．"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420282801193724160","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10314858"},{"@type":"NRID","@value":"1000010314858"},{"@type":"NRID","@value":"9000006261046"},{"@type":"NRID","@value":"9000003195396"},{"@type":"NRID","@value":"9000017712898"},{"@type":"NRID","@value":"9000403546175"},{"@type":"NRID","@value":"9000403296163"},{"@type":"NRID","@value":"9000391877666"},{"@type":"NRID","@value":"9000391904391"},{"@type":"NRID","@value":"9000391875349"},{"@type":"NRID","@value":"9000400189060"},{"@type":"NRID","@value":"9000391887451"},{"@type":"NRID","@value":"9000311066537"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/TR-Organosilica"}],"foaf:name":[{"@language":"en","@value":"Nakamura Michihiro"},{"@language":"ja","@value":"中村 教泰"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Anatomy and Cell Biology, Institute of Health Biosciences, The University of Tokushima Graduate School"},{"@language":"ja","@value":"徳島大学大学院　ヘルスバイオサイエンス部・顕微解剖学"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001204766964352","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Ishimura Kazunori"},{"@language":"ja","@value":"石村 和敬"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Anatomy and Cell Biology, Institute of Health Biosciences, The University of Tokushima Graduate School"},{"@language":"ja","@value":"徳島大学大学院　ヘルスバイオサイエンス部・顕微解剖学"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"02886200"},{"@type":"LISSN","@value":"02886200"},{"@type":"EISSN","@value":"18811639"},{"@type":"NCID","@value":"AN0035795X"}],"prism:publicationName":[{"@language":"en","@value":"The Journal of Japan Society for Laser Surgery and Medicine"},{"@language":"ja","@value":"日本レーザー医学会誌"},{"@language":"en","@value":"JJSLSM"},{"@language":"ja","@value":"日レ医誌"},{"@language":"en","@value":"Nippon Laser Igakkaishi"}],"dc:publisher":[{"@language":"en","@value":"Japan Society for Laser Surgery and Medicine"},{"@language":"ja","@value":"特定非営利活動法人 日本レーザー医学会"}],"prism:publicationDate":"2009","prism:volume":"30","prism:number":"4","prism:startingPage":"394","prism:endingPage":"398"},"reviewed":"false","url":[{"@id":"https://search.jamas.or.jp/link/ui/2010180377"}],"availableAt":"2009","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=organosilica%20nanoparticles","dc:title":"organosilica nanoparticles"},{"@id":"https://cir.nii.ac.jp/all?q=cytotoxicity","dc:title":"cytotoxicity"},{"@id":"https://cir.nii.ac.jp/all?q=imaging","dc:title":"imaging"},{"@id":"https://cir.nii.ac.jp/all?q=therapeutic%20particles.","dc:title":"therapeutic particles."},{"@id":"https://cir.nii.ac.jp/all?q=%E6%9C%89%E6%A9%9F%E3%82%B7%E3%83%AA%E3%82%AB%E3%83%8A%E3%83%8E%E7%B2%92%E5%AD%90","dc:title":"有機シリカナノ粒子"},{"@id":"https://cir.nii.ac.jp/all?q=%E7%B4%B0%E8%83%9E%E9%9A%9C%E5%AE%B3%E6%B4%BB%E6%80%A7","dc:title":"細胞障害活性"},{"@id":"https://cir.nii.ac.jp/all?q=%E3%82%A4%E3%83%A1%E3%83%BC%E3%82%B8%E3%83%B3%E3%82%B0","dc:title":"イメージング"},{"@id":"https://cir.nii.ac.jp/all?q=%E6%B2%BB%E7%99%82%E7%94%A8%E3%83%8A%E3%83%8E%E7%B2%92%E5%AD%90","dc:title":"治療用ナノ粒子"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282257052319744","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"21500409"},{"@type":"JGN","@value":"JP21500409"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21500409/"}],"notation":[{"@language":"ja","@value":"有機シリカ粒子技術による革新的マルチモーダルイメージングプローブの開発"},{"@language":"en","@value":"Development of innovative multimodal imaging probes using organosilica particle technology"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011145034652160","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"One-Pot Synthesis and Characterization of Three Kinds of Thiol−Organosilica Nanoparticles"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292620927310208","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Synthesis, Characterization, and Biological Applications of Multifluorescent Silica Nanoparticles"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262945084802432","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Organically Modified Silica Nanoparticles Co-encapsulating Photosensitizing Drug and Aggregation-Enhanced Two-Photon Absorbing Fluorescent Dye Aggregates for Two-Photon Photodynamic Therapy"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107368383380480","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Organically Modified 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