{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390001204900682112.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2220/biomedres.27.117"}},{"identifier":{"@type":"PMID","@value":"16847357"}},{"identifier":{"@type":"NAID","@value":"130004470657"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2007131304"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Nitric oxide synthase activity in rat gastric mucosa contributes to mucin synthesis elicited by calcitonin gene-related peptide"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"The majority of research for the calcitonin gene-related peptide (CGRP) in the stomach has been devoted to the submucosal blood flow, and only slight attention has been paid to its involvement in the gastric epithelial function. In this study, we examined the age-related change in the CGRPcontaining nerves and its effects on the mucus metabolism. We compared the immunoreactivity for CGRP in the gastric mucosa of 7-week-old rats (young) to that of 52-week-old animals (middle-aged). The effects of CGRP on the mucin biosynthesis were compared using the stomachs from both young and middle-aged rats. The nitric oxide synthase (NOS) activity was measured in the surface and deep mucosa of the gastric corpus. The density of the CGRP nerve fibers was reduced in both the lamina propria and submucosa of the middle-aged rats compared to the young rats. CGRP stimulated the mucin biosynthesis in the cultured corpus mucosa from the 7-week-old rats, but not from the 52-week-old rats. The total NOS activity of the surface layer in the corpus mucosa was markedly reduced in the middle-aged rats compared to the young rats. These findings demonstrate the age-dependent reduction in the CGRP-induced mucin biosynthesis, as well as in the density of the CGRP fibers in the rat stomach. The decreased NOS activity in the surface layer of the oxyntic mucosa in the aged rats may also be a principal cause for the lack of regulation of the mucin biosynthesis by CGRP."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420001326219127680","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"30245378"},{"@type":"NRID","@value":"1000030245378"},{"@type":"NRID","@value":"9000003072186"},{"@type":"NRID","@value":"9000391612797"},{"@type":"NRID","@value":"9000257922990"},{"@type":"NRID","@value":"9000356606134"},{"@type":"NRID","@value":"9000019065771"},{"@type":"NRID","@value":"9000283496347"},{"@type":"NRID","@value":"9000257923326"},{"@type":"NRID","@value":"9000278512211"},{"@type":"NRID","@value":"9000283496526"},{"@type":"NRID","@value":"9000257923067"},{"@type":"NRID","@value":"9000257923946"},{"@type":"NRID","@value":"9000408777166"},{"@type":"NRID","@value":"9000272995099"},{"@type":"NRID","@value":"9000272995028"},{"@type":"NRID","@value":"9000273022320"},{"@type":"NRID","@value":"9000412051483"},{"@type":"NRID","@value":"9000020366818"},{"@type":"NRID","@value":"9000257770547"},{"@type":"NRID","@value":"9000000724241"},{"@type":"NRID","@value":"9000107333367"},{"@type":"NRID","@value":"9000265566468"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0024344"}],"foaf:name":[{"@language":"en","@value":"ICHIKAWA Takafumi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Departments of Biochemistry, Kitasato University School of Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658352591745","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80117663"},{"@type":"NRID","@value":"1000080117663"},{"@type":"NRID","@value":"9000343347587"},{"@type":"NRID","@value":"9000343347325"},{"@type":"NRID","@value":"9000253266865"},{"@type":"NRID","@value":"9000403960781"},{"@type":"NRID","@value":"9000347073505"},{"@type":"NRID","@value":"9000008972172"},{"@type":"NRID","@value":"9000000344050"},{"@type":"NRID","@value":"9000343347524"},{"@type":"NRID","@value":"9000253266801"},{"@type":"NRID","@value":"9000283496346"},{"@type":"NRID","@value":"9000343347073"},{"@type":"NRID","@value":"9000343347381"},{"@type":"NRID","@value":"9000343347382"},{"@type":"NRID","@value":"9000402919452"},{"@type":"NRID","@value":"9000253266293"},{"@type":"NRID","@value":"9000392136589"},{"@type":"NRID","@value":"9000008432131"},{"@type":"NRID","@value":"9000343348225"},{"@type":"NRID","@value":"9000380967563"},{"@type":"NRID","@value":"9000253045990"},{"@type":"NRID","@value":"9000283496530"},{"@type":"NRID","@value":"9000257923068"},{"@type":"NRID","@value":"9000343347457"},{"@type":"NRID","@value":"9000414725041"},{"@type":"NRID","@value":"9000253266578"},{"@type":"NRID","@value":"9000020198856"},{"@type":"NRID","@value":"9000343347199"},{"@type":"NRID","@value":"9000392137674"},{"@type":"NRID","@value":"9000343347588"},{"@type":"NRID","@value":"9000343347161"},{"@type":"NRID","@value":"9000343347257"},{"@type":"NRID","@value":"9000347045903"},{"@type":"NRID","@value":"9000343347458"},{"@type":"NRID","@value":"9000022013936"},{"@type":"NRID","@value":"9000347045520"},{"@type":"NRID","@value":"9000347070080"}],"foaf:name":[{"@language":"en","@value":"KUSAKABE Tatsumi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Departments of Sport and Medical Science, Kokushikan University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001204900681984","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000257923070"}],"foaf:name":[{"@language":"en","@value":"SHIKAMA Nobuaki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Departments of Internal Medicine, Kitasato University School of Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001204900682114","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000257923069"}],"foaf:name":[{"@language":"en","@value":"GONO Yukari"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Departments of Internal Medicine, Kitasato University School of Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658355736704","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10238397"},{"@type":"NRID","@value":"1000010238397"},{"@type":"NRID","@value":"9000257923071"},{"@type":"NRID","@value":"9000021859754"},{"@type":"NRID","@value":"9000008973534"},{"@type":"NRID","@value":"9000001546671"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0161109"}],"foaf:name":[{"@language":"en","@value":"HIRUMA Hiromi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Departments of Physiology, Kitasato University School of Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658352591744","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"60177649"},{"@type":"NRID","@value":"1000060177649"},{"@type":"NRID","@value":"9000003720667"},{"@type":"NRID","@value":"9000363349715"},{"@type":"NRID","@value":"9000253266866"},{"@type":"NRID","@value":"9000256311766"},{"@type":"NRID","@value":"9000401651697"},{"@type":"NRID","@value":"9000000913833"},{"@type":"NRID","@value":"9000007149283"},{"@type":"NRID","@value":"9000253266802"},{"@type":"NRID","@value":"9000343346889"},{"@type":"NRID","@value":"9000257923072"},{"@type":"NRID","@value":"9000392728574"},{"@type":"NRID","@value":"9000253266579"},{"@type":"NRID","@value":"9000252978349"},{"@type":"NRID","@value":"9000343346827"},{"@type":"NRID","@value":"9000258132218"},{"@type":"NRID","@value":"9000006099923"},{"@type":"NRID","@value":"9000258707955"},{"@type":"NRID","@value":"9000283525754"},{"@type":"NRID","@value":"9000255894614"},{"@type":"NRID","@value":"9000415398646"},{"@type":"NRID","@value":"9000401655912"},{"@type":"NRID","@value":"9000005549724"},{"@type":"NRID","@value":"9000253319479"},{"@type":"NRID","@value":"9000401625966"},{"@type":"NRID","@value":"9000256259711"},{"@type":"NRID","@value":"9000343346626"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0019983"}],"foaf:name":[{"@language":"en","@value":"KAWAKAMI Tadashi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Departments of Physiology, Kitasato University School of Medicine"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658332769664","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"10104530"},{"@type":"NRID","@value":"1000010104530"},{"@type":"NRID","@value":"9000253178782"},{"@type":"NRID","@value":"9000272994816"},{"@type":"NRID","@value":"9000257922996"},{"@type":"NRID","@value":"9000283496348"},{"@type":"NRID","@value":"9000257923330"},{"@type":"NRID","@value":"9000253182941"},{"@type":"NRID","@value":"9000257923073"},{"@type":"NRID","@value":"9000272995105"},{"@type":"NRID","@value":"9000273022325"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0044237"}],"foaf:name":[{"@language":"en","@value":"ISHIHARA Kazuhiko"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Departments of Biochemistry, Kitasato University School of Allied Health Sciences"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"03886107"},{"@type":"LISSN","@value":"03886107"},{"@type":"EISSN","@value":"1880313X"}],"prism:publicationName":[{"@language":"en","@value":"Biomedical Research"},{"@language":"en","@value":"Biomed. Res."}],"dc:publisher":[{"@language":"en","@value":"Biomedical Research Press"},{"@language":"ja","@value":"バイオメディカルリサーチプレス"}],"prism:publicationDate":"2006","prism:volume":"27","prism:number":"3","prism:startingPage":"117","prism:endingPage":"124"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"https://search.jamas.or.jp/link/ui/2007131304"}],"availableAt":"2006","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=Male","dc:title":"Male"},{"@id":"https://cir.nii.ac.jp/all?q=Time%20Factors","dc:title":"Time Factors"},{"@id":"https://cir.nii.ac.jp/all?q=Calcitonin%20Gene-Related%20Peptide","dc:title":"Calcitonin Gene-Related Peptide"},{"@id":"https://cir.nii.ac.jp/all?q=Mucins","dc:title":"Mucins"},{"@id":"https://cir.nii.ac.jp/all?q=Cesium","dc:title":"Cesium"},{"@id":"https://cir.nii.ac.jp/all?q=Immunohistochemistry","dc:title":"Immunohistochemistry"},{"@id":"https://cir.nii.ac.jp/all?q=Rats","dc:title":"Rats"},{"@id":"https://cir.nii.ac.jp/all?q=Chlorides","dc:title":"Chlorides"},{"@id":"https://cir.nii.ac.jp/all?q=Gastric%20Mucosa","dc:title":"Gastric Mucosa"},{"@id":"https://cir.nii.ac.jp/all?q=Animals","dc:title":"Animals"},{"@id":"https://cir.nii.ac.jp/all?q=Nitric%20Oxide%20Synthase","dc:title":"Nitric Oxide Synthase"},{"@id":"https://cir.nii.ac.jp/all?q=Rats,%20Wistar","dc:title":"Rats, Wistar"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000781815114240","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"15590683"},{"@type":"JGN","@value":"JP15590683"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15590683/"}],"notation":[{"@language":"ja","@value":"神経網形成を促す神経栄養因子が消化管粘膜の恒常性維持及び損傷修復に果す役割の追究"},{"@language":"en","@value":"Neurotrophic factors and gastrointestinal mucosa"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282256861537536","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"16590629"},{"@type":"JGN","@value":"JP16590629"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16590629/"}],"notation":[{"@language":"ja","@value":"単クローン抗体で認識される消化管粘液中の酸性糖鎖の生体内における機能の研究"},{"@language":"en","@value":"Function of gastro-intestinal mucin-derived acidic oligosaccharaides recognized by anti-mucin monoclonal antibodies"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011144333316992","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A separating method for quantifying mucus glycoprotein localized in the different layer of rat gastric mucosa"}]},{"@id":"https://cir.nii.ac.jp/crid/1360011144994878208","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Peptide-containing nerve fibers in the stomach wall of rat and mouse"}]},{"@id":"https://cir.nii.ac.jp/crid/1360292619085279872","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Characterization of the peptidergic afferent innervation of the stomach in the rat, mouse and guinea-pig"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855568951048064","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Calcitonin gene-related peptide affords gastric mucosal protection by activating potassium channel in Wistar rat"}]},{"@id":"https://cir.nii.ac.jp/crid/1360855569674691712","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Incidence of Gastric Ulcer in Copenhagen County"}]},{"@id":"https://cir.nii.ac.jp/crid/1361137046267088384","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Influence of age on the signal transduction pathway of non‐adrenergic non‐cholinergic neurotransmitters in the rat gastric fundus"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418518533215488","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Localization of substance P, CGRP, VIP, neuropeptide Y, and somatostatin immunoreactive nerve fibers in the carotid labyrinths of some amphibian species"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418520608639872","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Distribution of certain peptide‐containing nerve fibres and endocrine cells in the gastrointestinal mucosa in five mammalian species"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418520632835072","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"CGRP modulates mucin synthesis in surface  mucus cells of rat gastric oxyntic mucosa"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418521265164416","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Distribution and characterization of calcitonin gene-related peptide immunoreactivity in the digestive system of normal and capsaicin-treated rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418521385204224","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Morphologic changes in gastric mucosa of aging rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1361418521408717824","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effect of CGRP antagonist, alpha-CGRP 8-37, on acid secretion in the dog"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981471086588160","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effect of colloidal bismuth subcitrate on age related gastric lesions in the rat."}]},{"@id":"https://cir.nii.ac.jp/crid/1362262944277047168","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"CGRP antagonists enhance gastric acid secretion in 2-h pylorus-ligated rats"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544419146604160","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effects of acid-inhibitory antiulcer drugs on mucin biosynthesis in the rat stomach"}]},{"@id":"https://cir.nii.ac.jp/crid/1362544420456283392","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Role of gastric blood flow in impaired defense and repair of aged rat stomachs"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825893360120192","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Stimulation of mucus glycoprotein biosynthesis in rat gastric mucosa by gastrin"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825893916877696","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Age-related stimulation by tetragastrin of gastric mucin biosynthesis in rat"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894040273152","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Localization of constitutive isoforms of nitric oxide synthase in the gastric glandular mucosa of the rat"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825894610914432","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"The Role of Calcitonin Gene-Related Peptide and Nitric Oxide in Gastric Mucosal Hyperemia and Protection"}]},{"@id":"https://cir.nii.ac.jp/crid/1362825895155005440","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Increased susceptibility of rat gastric mucosa to ulcerogenic stimulation with aging"}]},{"@id":"https://cir.nii.ac.jp/crid/1363107370673312896","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Effects of Several Denervation Procedures on Distribution of Calcitonin Gene-Related Peptide and Substance P Immunoreactive in Rat Stomach"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388843836065280","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Fundic Atrophic Gastritis in an Elderly Population: Effect on Hemoglobin and Several Serum Nutritional Indicators"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388844669454336","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Isolation of Large Gel-Forming Mucins"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388845077200000","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Role of calcitonin gene-related peptide and nitric oxide in the gastroprotective effect of capsaicin in the rat"}]},{"@id":"https://cir.nii.ac.jp/crid/1363388846217677312","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mucin Biophysics"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670318333479808","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Sensory neurons mediate protective vasodilatation in rat gastric mucosa"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320447805184","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Neural emergency system in the stomach"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951793725601408","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Types of oligosaccharide sulphation, depending on mucus glycoprotein source, corpus or antral, in rat stomach"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951793881886720","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Nitric oxide mediates glutamate-linked enhancement of cGMP levels in the cerebellum."}]},{"@id":"https://cir.nii.ac.jp/crid/1363951794142553344","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Isolation and partial characterization of rat gastric mucous glycoprotein"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951794662760192","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Stimulant effect of nitric oxide generator and roxatidine on mucin biosynthesis of rat gastric oxyntic mucosa"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233270113592576","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Age-Dependent Changes of NO Synthase Activity in the Rat Brain"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001204900274944","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@language":"en","@value":"<b>TOPOGRAPHICAL DISTRIBUTION OF NEUROPEPTIDE-CONTAINING NERVE FIBERS IN THE RAT STOMACH: A QUANTITATIVE ANALYSIS USING ANTIBODIES AGAINST PROTEIN GENE PRODUCT 9.5 AND GASTRIC </b><b>MUCINS </b>"},{"@value":"TOPOGRAPHICAL DISTRIBUTION OF NEUROPEPTIDE-CONTAINING NERVE FIBERS IN THE RAT STOMACH: A QUANTITATIVE ANALYSIS USING ANTIBODIES AGAINST PROTEIN GENE PRODUCT 9.5 AND GASTRIC MUCINS"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0028149036"},{"@type":"CROSSREF","@value":"10.2220/biomedres.27.117"},{"@type":"PUBMED","@value":"16847357"},{"@type":"CIA","@value":"130004470657"},{"@type":"KAKEN","@value":"PRODUCT-18564640"},{"@type":"KAKEN","@value":"PRODUCT-18564666"},{"@type":"KAKEN","@value":"PRODUCT-18835263"},{"@type":"OPENAIRE","@value":"doi_dedup___::b249187820e55efe446e015cd228b433"}]}