Conformational Difference in HMGB 1 Proteins of Human Neutrophils and Lymphocytes Revealed by Epitope Mapping of a Monoclonal Antibody
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- Ito Ichiaki
- Department of Biological Science and Technology, Science University of Tokyo
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- Mitsuoka Nobuyoshi
- Department of Biological Science and Technology, Science University of Tokyo
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- Sobajima Junko
- Department of Rheumatology and Clinical Immunology, Kyoto University Graduate School of Medicine
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- Uesugi Hiroko
- Saiseikai Noe Hospital
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- Ozaki Shoichi
- Division of Rheumatology and Allergy, Department of Internal Medicine, St. Marianna University School of Medicine
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- Ohya Kazuhiko
- Department of Product Development, Medical and Biological Laboratories Company, Ltd.
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- Yoshida Michiteru
- Department of Biological Science and Technology, Science University of Tokyo
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抄録
HMGB 1 and HMGB 2 are abundant nonhistone chromosomal proteins in eukaryotic organisms. Their respective primary sequences are highly conserved. Our previous studies showed that these proteins are novel autoantigens of anti-neutrophil cytoplasmic antibodies in sera from patients with ulcerative colitis (UC), rheumatic disease and autoimmune hepatitis (AIH). In the present paper, we showed that antiHMGB 1 and HMGB 2 antibodies in sera of patients with UC do not recognize HMGB 1 in neutrophils while they recognize the protein in lymphocytes. Anti-HMGB 2 mono-clonal antibody FBH 7, recognizing HMGB 1 in lymphocytes, showed a similar profile to the antibodies in the patients' sera. In order to elucidate the difference in immuno-reactivity to HMGB 1 between neutrophils and lymphocytes, we mapped the epitope for FBH 7 by means of several methods. The results showed that FBH 7 recognizes the intact conformation composed of 52-56 residues of HMGB 1 in lymphocytes. This suggested that HMGB 1 in neutrophils is conformationally changed in the epitope or the peripheral structure of the epitope from the protein in lymphocytes. The apparent conformational change of HMGB 1 between neutrophils and lymphocytes will be important for understanding the functional difference of HMGBl in these cells.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 136 (2), 155-162, 2004
社団法人 日本生化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204966049408
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- NII論文ID
- 130003418159
- 10016201297
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- NII書誌ID
- AA00694073
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- COI
- 1:CAS:528:DC%2BD2cXpslKrs7Y%3D
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- ISSN
- 17562651
- 0021924X
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- NDL書誌ID
- 7068327
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- PubMed
- 15496585
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可