Sarcoplasmic Reticulum Ca2+ Content Affects 4-CmC and Caffeine Contractures of Rat Skinned Skeletal Muscle Fibers.
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- Choisy Stéphanie
- Laboratoire de Physiologie Générale, CNRS UMR 6018, Faculté des Sciences et des Techniques de Nantes
Bibliographic Information
- Other Title
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- Sarcoplasmic Reticulum Ca〔2+〕 Content Affects 4-CmC and Caffeine Contractures of Rat Skinned SkeletaI Muscle Fibers
- Sarcoplasmic Reticulum Ca 2 Content Affects 4 CmC and Caffeine Contractures of Rat Skinned SkeletaI Muscle Fibers
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Description
This study investigated whether the sarcoplasmic reticulum Ca2+ content of rat skeletal muscle fibers affected contractile responses obtained by an application of 4-chloro-m-cresol (4-CmC) and caffeine. Contractures were elicited on saponin-skinned fibers under different Ca2+ loading conditions. The amplitude of 4-CmC and caffeine contractures of fast-twitch muscle fibers (edl, extensor digitorum longus) differed between the different loading conditions, and this is associated with a greater change in sensitivity to 4-CmC. When the sarcoplasmic reticulum was loaded with a low Ca2+ concentration for a short period, the 4-CmC concentration providing half-maximal response was tenfold higher than with a larger sarcoplasmic reticulum Ca2+ loading for a longer period, whereas for caffeine this concentration was only twofold higher in the same conditions. These findings indicate that 4-CmC contractile responses of edl muscle fibers are more dependent on luminal Ca2+ activity than those of caffeine are. Thus 4-CmC would appear to be of greater interest than caffeine for the study of muscle contractile responses where variations in intracellular Ca2+ activity exist.<br>
Journal
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- The Japanese Journal of Physiology
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The Japanese Journal of Physiology 51 (6), 661-669, 2001
THE PHYSIOLOGICAL SOCIETY OF JAPAN
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Details 詳細情報について
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- CRID
- 1390001205043679744
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- NII Article ID
- 130004435801
- 50001044548
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- NII Book ID
- AA00691224
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- COI
- 1:CAS:528:DC%2BD38XitVCrsro%3D
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- ISSN
- 18811396
- 0021521X
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- NDL BIB ID
- 6069526
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- PubMed
- 11846956
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed