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Molecular Epidemiological Investigation of Vero toxin-producing <I>Escherichia coli</I>(VTEC) Isolated from Diarrhea Patients and Dairy Cattle

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Other Title
  • 人下痢症および乳牛から分離されたVero毒素産生性大腸菌 (VTEC) の分子疫学的検討
  • ヒト ゲリショウ オヨビ ニュウギュウ カラ ブンリ サレタ Vero ドクソ サンセイセイ ダイチョウキン VTEC ノ ブンシ エキガクテキ ケントウ
  • Molecular Epidemiological Investigation of Vero toxin-producing Escherichia coli(VTEC) Isolated from Diarrhea Patients and Dairy Cattle

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Abstract

To elucidate the source and route of VTEC infection, we performed pulse field gel electrophoresis (PFGE) an 50 isolates from human diarrhea typed as serotypes O157, O111, and O26, which were very frequently isolated from patients with VTEC infection between 1986 and 1997, and 32 isolates from dairy cattle, a total of 82 isolates. The isolates were genetically analyzed based on the electrophoresis patterns of DNA, and a phylogenetic tree was prepared. The isolates were classified based on similarity≥89. The following results of the molecular epidemiological investigation were obtained.<BR>1) Based on the electrophoresis patterns of DNA obtained by PFGE, 34 of the 49 0157 isolates (69.4%) were divided into groups 1-9, 15 of the 18 O111 isolates (83.3%) were divided into groups 1-3, and 12 of the 15 026 isolates (80%) were divided into groups 1-3. Of the grouped isolates, group 8 of 0157, groups 2 and 3 of 0111, and group 3 of 026 included isolates from human diarrhea and dairy cattle, but the other groups included isolates from only one of the two sources.<BR>2) With regard to regional investigation, groups 6 and 9 of O157 included human diarrhea-derived isolates from Yokohama and Ehime, and group 8 included a human diarrhea-derived isolate from Yokohama and a dairy cattle-derived isolate from Tokushima. Group 3 of 0111 included a human diarrhea-derived isolate from Ehime and a dairy cattle-derived isolate from Hokkaido. Group 3 of 026 included human diarrhea-derived isolate from Ehime and dairy cattle-derived isolate from Sagamihara and Hokkaido.<BR>Since the above findings showed that although the frequency was low, isolates from human diarrhea and dairy cattle were included in the same groups, it was demonstrated that dairy cattle are closely related to the human infectious disease of the intestinal tract as a source of infection. However, classification using the PFGE method is difficult due to diversity of the electrophoresis pattern of DNA. It is necessary to investigate the classification by a combination of the PFGE method with phage typing, ribotyping, and RAPD-PCR, and to investigate more numbers of patient-derived and animal-derived isolates.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 76 (2), 96-101, 2002

    The Japanese Association for Infectious Diseases

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