A Possible Procedure to Prepare Mono Fatty Acid-Enriched Plasma Membrane in Chicken Hepatocytes

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  • TAKAHASHI Toshihiro
    Department of Animal Science, Faculty of Agriculture, Tohoku University
  • SATO Kan
    Department of Animal Science, Faculty of Agriculture, Tohoku University
  • AKIBA Yukio
    Department of Animal Science, Faculty of Agriculture, Tohoku University

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  • Possible Procedure to Prepare Mono Fatty Acid-Enriched Plasma Membrane in Chicken Hepatocytes

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In a viewpoint to explore an experimental model to study the regulatory mechanism of hepatic lipogenesis by n-3 fatty acids on hepatic lipogenesis, three experiments were undertaken to prepare mono fatty acid-enriched plasma membrane phospholipid in chicken hepatocytes. Incubation with 500μM α-linolenic acid for 24h in the primary culture of chicken hepatocytes significantly increased α-linolenic acid content and decreased oleic acid content in the plasma membrane phospholipid. In a same fashion, addition of linoleic acid or oleic acid to the media resulted in the higher proportion of the corresponding fatty acid in the plasma membrane. In the subsequent incubation in a fatty acid-free media of α-linolenic acid-enriched cells, α-linolenic acid content decreased gradually with a half life of 24h. Our results suggest that fatty acid supplementation to the culture medium modifies the fatty acid composition of plasma membrane phospholipid of chicken hepatocytes. The present chicken primary culture system may be applicable for investigation of regulatory mechanism of lipid metabolism.

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