{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390001205208758528.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.1584/jpestics.7.427"}},{"identifier":{"@type":"URI","@value":"https://agriknowledge.affrc.go.jp/RN/2010254022"}},{"identifier":{"@type":"NDL_BIB_ID","@value":"2461348"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/bib/2461348"}},{"identifier":{"@type":"URI","@value":"https://ndlsearch.ndl.go.jp/books/R000000004-I2461348"}},{"identifier":{"@type":"URI","@value":"https://dl.ndl.go.jp/pid/10873897"}},{"identifier":{"@type":"NAID","@value":"110001713722"}},{"identifier":{"@type":"URI","@value":"http://id.ndl.go.jp/digimeta/10873897"}},{"identifier":{"@type":"URI","@value":"http://www.jstage.jst.go.jp/article/jpestics1975/7/3/7_3_427/_pdf"}},{"identifier":{"@type":"URI","@value":"http://dl.ndl.go.jp/info:ndljp/pid/10873897"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Mechanism of Action of the Fungicide Buthiobate"},{"@language":"ja","@value":"殺菌剤ブチオベートの作用機構"},{"@language":"ja-Kana","@value":"サッキンザイ ブチオベート ノ サヨウ キコウ"}],"description":[{"type":"abstract","notation":[{"@language":"en","@value":"Buthiobate is effective in controlling powdery mildews of agricultural and ornamental crops. Microscopic observations indicated that the fungicide caused abnormal swelling of germ tubes of <i>Sphaerotheca fuliginea</i> and excessive branching of hyphae in <i>Monilinia fructigena</i> without inhibition of spore germination. Respiratory activity and the incorporations of radioactive substrates into proteins, nucleic acids and cell wall were not significantly inhibited in <i>M. fructigena</i> during early incubation periods with the fungicide. On the contrary, sterol biosynthesis was severely affected by the treatment. By gas-liquid chromatographic analyses, it was shown that an amount of ergosterol decreased in the treated culture with a concomitant accumulation of 24-methylenedihydrolanosterol and obtusifoliol. In cell-free homogenates of <i>Saccharomyces cerevisiae</i>, the conversion of acetate-U-<sup>14</sup><i>C</i> to 4-desmethyl sterols was also proved to be suppressed by the fungicide and radioactivity accumulated in the fraction of lanosterol. <sup>14</sup><i>C</i>-Labelled lanosterol was not converted to 4-desmethyl sterols in the presence of the fungicide, whereas the conversion of <sup>14</sup><i>C</i>-labelled 14-desmethyl-lanosterol was not inhibited. The results therefore indicate selective inhibition of demethylation at C-14 in the biosynthetic processes of ergosterol. The fungicide tridemorph was also found to be an inhibitor of ergosterol biosynthesis in <i>Botrytis cinerea</i>. Since fecosterol, ergosta-8, 22, 24(28)-trien-3β-ol and ergosta-8, 22-dien-3β-ol accumulated in the treated culture, the fungicide seemed to block the conversion of fecosterol to episterol, i. e. the Δ<sup>8</sup>→Δ<sup>7</sup> isomerization."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410009221074193923","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000256381828"},{"@type":"NRID","@value":"9000256382243"},{"@type":"NRID","@value":"9000003009083"},{"@type":"NRID","@value":"9000256382391"},{"@type":"NRID","@value":"9000256382725"},{"@type":"NRID","@value":"9000256382666"},{"@type":"NRID","@value":"9000256379115"},{"@type":"NRID","@value":"9000256382594"},{"@type":"NRID","@value":"9000256382082"},{"@type":"NRID","@value":"9000256382674"},{"@type":"NRID","@value":"9000255816068"},{"@type":"NRID","@value":"9000256378711"},{"@type":"NRID","@value":"9000256382628"},{"@type":"NRID","@value":"9000256379209"},{"@type":"NRID","@value":"9000256382658"},{"@type":"NRID","@value":"9000256382366"},{"@type":"NRID","@value":"9000255814447"},{"@type":"NRID","@value":"9000256382573"},{"@type":"NRID","@value":"9000256382664"},{"@type":"NRID","@value":"9000256379246"}],"foaf:name":[{"@language":"en","@value":"KATO Toshiro"},{"@language":"ja","@value":"加藤 寿郎"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Research Department, Pesticides Division, Sumitomo Chemical Co., Ltd."},{"@language":"ja","@value":"住友化学工業株式会社農薬事業部研究部"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"1348589X"},{"@type":"EISSN","@value":"13490923"},{"@type":"CODEN","@value":"JPSTCF"},{"@type":"NDL_BIB_ID","@value":"000000028711"},{"@type":"ISSN","@value":"03851559"},{"@type":"LISSN","@value":"03851559"},{"@type":"NCID","@value":"AN00196227"}],"prism:publicationName":[{"@language":"en","@value":"Journal of Pesticide Science"},{"@language":"ja","@value":"日本農薬学会誌（Ｊｏｕｒｎａｌ　ｏｆ　Ｐｅｓｔｉｃｉｄｅ　Ｓｃｉｅｎｃｅ）"},{"@language":"en","@value":"J. Pestic. Sci."},{"@language":"en","@value":"Journal of Pesticide Science"},{"@language":"ja","@value":"日本農薬学会誌（Ｊｏｕｒｎａｌ　ｏｆ　Ｐｅｓｔｉｃｉｄｅ　Ｓｃｉｅｎｃｅ）"}],"dc:publisher":[{"@language":"en","@value":"Pesticide Science Society of Japan"},{"@language":"ja","@value":"日本農薬学会"}],"prism:publicationDate":"1982","prism:volume":"7","prism:number":"3","prism:startingPage":"427","prism:endingPage":"437"},"reviewed":"false","url":[{"@id":"https://agriknowledge.affrc.go.jp/RN/2010254022"},{"@id":"http://id.ndl.go.jp/bib/2461348"},{"@id":"https://ndlsearch.ndl.go.jp/books/R000000004-I2461348"},{"@id":"https://dl.ndl.go.jp/pid/10873897"},{"@id":"http://id.ndl.go.jp/digimeta/10873897"},{"@id":"http://www.jstage.jst.go.jp/article/jpestics1975/7/3/7_3_427/_pdf"},{"@id":"http://dl.ndl.go.jp/info:ndljp/pid/10873897"}],"availableAt":"1982","dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0036538718"},{"@type":"IRDB","@value":"oai:irdb.nii.ac.jp:03420:0004152486"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R000000004-I2461348"},{"@type":"NDL_SEARCH","@value":"oai:ndlsearch.ndl.go.jp:R100000039-I10873897"},{"@type":"CROSSREF","@value":"10.1584/jpestics.7.427"},{"@type":"NDL_DC","@value":"info:ndljp/pid/10873897"},{"@type":"CIA","@value":"110001713722"}]}