{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390001205221374080.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2142/biophysics.9.123"}},{"identifier":{"@type":"COI","@value":"1:CAS:528:DC%2BC3sXhsVegu7jM"}},{"identifier":{"@type":"URI","@value":"https://www.jstage.jst.go.jp/article/biophysics/9/0/9_123/_pdf"}},{"identifier":{"@type":"PMID","@value":"27493550"}},{"identifier":{"@type":"NAID","@value":"130003366039"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"Development of a rapid Buffer-exchange system for time-resolved ATR-FTIR spectroscopy with the step-scan mode"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"Attenuated total reflectance (ATR)-FTIR spectroscopy has been widely used to probe protein structural changes under various stimuli, such as light absorption, voltage change, and ligand binding, in aqueous conditions. Time-resolved measurements require a trigger, which can be controlled electronically; therefore, light and voltage changes are suitable. Here we developed a novel, rapid buffer-exchange system for time-resolved ATR-FTIR spectroscopy to monitor the ligand- or ion-binding reaction of a protein. By using the step-scan mode (time resolution; 2.5 ms), we confirmed the completion of the buffer-exchange reaction within ~25 ms; the process was monitored by the infrared absorption change of a nitrate band at 1,350 cm<sup>-1</sup>. We also demonstrated the anion-binding reaction of a membrane protein, <i>Natronomonas pharaonis</i> halorhodopsin (<i>p</i>HR), which binds a chloride ion in the initial anion-binding site near the retinal chromophore. The formation of chloride- or nitrate-bound <i>p</i>HR was confirmed by an increase of the retinal absorption band at 1,528 cm<sup>-1</sup>. It also should be noted that low sample consumption (~1 μg of protein) makes this new method a powerful technique to understand ligand-protein and ion-protein interactions, particularly for membrane proteins.<br>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420282801190639616","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"80432285"},{"@type":"NRID","@value":"1000080432285"},{"@type":"NRID","@value":"9000018773692"},{"@type":"NRID","@value":"9000006332124"},{"@type":"NRID","@value":"9000238282285"},{"@type":"NRID","@value":"9000017712757"},{"@type":"NRID","@value":"9000006868234"},{"@type":"NRID","@value":"9000294356641"},{"@type":"NRID","@value":"9000238280725"},{"@type":"NRID","@value":"9000259304939"},{"@type":"NRID","@value":"9000238279830"},{"@type":"NRID","@value":"9000238280399"},{"@type":"NRID","@value":"9000303642466"},{"@type":"NRID","@value":"9000390407476"},{"@type":"NRID","@value":"9000362823800"},{"@type":"NRID","@value":"9000006866716"},{"@type":"NRID","@value":"9000331449273"},{"@type":"NRID","@value":"9000304619613"},{"@type":"NRID","@value":"9000017712072"},{"@type":"NRID","@value":"9000399805106"},{"@type":"NRID","@value":"9000238282998"},{"@type":"NRID","@value":"9000238282291"},{"@type":"NRID","@value":"9000240058858"},{"@type":"NRID","@value":"9000255702237"},{"@type":"NRID","@value":"9000024407860"},{"@type":"NRID","@value":"9000403597059"},{"@type":"NRID","@value":"9000018808015"},{"@type":"NRID","@value":"9000259304852"},{"@type":"NRID","@value":"9000259304858"},{"@type":"NRID","@value":"9000303640943"},{"@type":"NRID","@value":"9000261656059"},{"@type":"NRID","@value":"9000370759268"},{"@type":"NRID","@value":"9000238279832"},{"@type":"NRID","@value":"9000238279923"},{"@type":"NRID","@value":"9000265246833"},{"@type":"NRID","@value":"9000002047082"},{"@type":"NRID","@value":"9000017714993"},{"@type":"NRID","@value":"9000315141791"},{"@type":"NRID","@value":"9000018808034"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0069998"}],"foaf:name":[{"@language":"en","@value":"Furutani Yuji"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science"},{"@language":"en","@value":"Department of Structural Molecular Science, The Graduate University for Advanced Studies (SOKENDAI)"}]},{"@id":"https://cir.nii.ac.jp/crid/1420282801211805952","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"70506906"},{"@type":"NRID","@value":"1000070506906"},{"@type":"NRID","@value":"9000002740115"},{"@type":"NRID","@value":"9000238282281"},{"@type":"NRID","@value":"9000294356639"},{"@type":"NRID","@value":"9000399009156"},{"@type":"NRID","@value":"9000259304936"},{"@type":"NRID","@value":"9000412182460"},{"@type":"NRID","@value":"9000237872607"},{"@type":"NRID","@value":"9000399009441"},{"@type":"NRID","@value":"9000412001761"},{"@type":"NRID","@value":"9000331449271"},{"@type":"NRID","@value":"9000238282288"},{"@type":"NRID","@value":"9000240058859"},{"@type":"NRID","@value":"9000366505995"},{"@type":"NRID","@value":"9000259304313"},{"@type":"NRID","@value":"9000018807163"},{"@type":"NRID","@value":"9000303641792"},{"@type":"NRID","@value":"9000409645927"},{"@type":"NRID","@value":"9000329418313"},{"@type":"NRID","@value":"9000347203997"},{"@type":"NRID","@value":"9000017714492"},{"@type":"NRID","@value":"9000399009181"},{"@type":"NRID","@value":"9000414090190"},{"@type":"NRID","@value":"9000411276068"},{"@type":"NRID","@value":"9000259304853"},{"@type":"NRID","@value":"9000303640413"},{"@type":"NRID","@value":"9000370759267"},{"@type":"NRID","@value":"9000238279831"},{"@type":"NRID","@value":"9000265246834"},{"@type":"NRID","@value":"9000382215655"},{"@type":"NRID","@value":"9000018808030"},{"@type":"NRID","@value":"9000412274331"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/Tetsunari-Kimura"}],"foaf:name":[{"@language":"en","@value":"Kimura Tetsunari"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science"},{"@language":"en","@value":"Department of Structural Molecular Science, The Graduate University for Advanced Studies (SOKENDAI)"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001205221374082","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000240058860"}],"foaf:name":[{"@language":"en","@value":"Okamoto Kido"}],"jpcoar:affiliationName":[{"@language":"en","@value":"UNISOKU Co., Ltd."}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"13492942"}],"prism:publicationName":[{"@language":"en","@value":"BIOPHYSICS"},{"@language":"en","@value":"BIOPHYSICS"}],"dc:publisher":[{"@language":"en","@value":"The Biophysical Society of Japan"},{"@language":"ja","@value":"日本生物物理学会"}],"prism:publicationDate":"2013","prism:volume":"9","prism:number":"0","prism:startingPage":"123","prism:endingPage":"129"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"https://www.jstage.jst.go.jp/article/biophysics/9/0/9_123/_pdf"}],"availableAt":"2013","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=membrane%20protein","dc:title":"membrane protein"},{"@id":"https://cir.nii.ac.jp/all?q=ion-protein%20interaction","dc:title":"ion-protein interaction"},{"@id":"https://cir.nii.ac.jp/all?q=halorhodopsin","dc:title":"halorhodopsin"},{"@id":"https://cir.nii.ac.jp/all?q=attenuated%20total%20reflectance","dc:title":"attenuated total reflectance"},{"@id":"https://cir.nii.ac.jp/all?q=stopped-flow%20technique","dc:title":"stopped-flow technique"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040000782178725632","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"23687022"},{"@type":"JGN","@value":"JP23687022"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23687022/"}],"notation":[{"@language":"ja","@value":"低収量生体分子の時間分解計測を目指したマイクロ流体ミキサーの開発"},{"@language":"en","@value":"Development of microfluidic mixer for time-resolved spectroscopy applicable to low-yield biomolecules"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360011145030782336","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Ultrafast Pump−Probe Study of the Primary Photoreaction Process in <i>pharaonis</i> Halorhodopsin: Halide Ion Dependence and Isomerization Dynamics"}]},{"@id":"https://cir.nii.ac.jp/crid/1360283692106685312","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Dehydration of 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The Photochemical Cycle"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670319549460992","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Selective detection of the structural changes upon photoreactions of several redox cofactors in photosystem II by means of light-induced ATR-FTIR difference spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670320074549760","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Cooperative three-step motions in catalytic subunits of F1-ATPase correlate with 80° and 40° substep rotations"}]},{"@id":"https://cir.nii.ac.jp/crid/1363951795528417664","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Stopped-Flow Analysis on Anion Binding to Blue-Form Halorhodopsin from <i>Natronobacterium pharaonis</i>:  Comparison with the Anion-Uptake Process during the Photocycle"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233269992580992","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Mixing liquids in microseconds"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233270916579072","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Methods to probe protein transitions with ATR infrared spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233270934785664","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Resolving voltage-dependent structural changes of a membrane photoreceptor by surface-enhanced IR difference spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205282052992","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Molecular Mechanisms of Membrane Proteins Studied by Infrared Spectroscopy"},{"@language":"ja","@value":"赤外分光法による膜タンパク質の分子機構研究"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001205763161344","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Specific interactions between alkali metal cations and the KcsA channel studied using ATR-FTIR spectroscopy"}]},{"@id":"https://cir.nii.ac.jp/crid/1390001206535880448","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Development of Rapid-Buffer Exchange ATR-FTIR Spectroscopy for Membrane Proteins"},{"@language":"ja","@value":"急速緩衝液交換法による時間分解全反射赤外分光法の開発"},{"@value":"理論/実験 技術 急速緩衝液交換法による時間分解全反射赤外分光法の開発"},{"@language":"ja-Kana","@value":"リロン/ジッケン ギジュツ キュウソク カンショウエキ コウカンホウ ニ ヨル ジカン ブンカイ ゼン ハンシャ セキガイ ブンコウホウ ノ カイハツ"}]},{"@id":"https://cir.nii.ac.jp/crid/1390282680198060672","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Mapping of the local environmental changes in proteins by cysteine scanning"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:1002220707"},{"@type":"CROSSREF","@value":"10.2142/biophysics.9.123"},{"@type":"CIA","@value":"130003366039"},{"@type":"KAKEN","@value":"PRODUCT-14313518"},{"@type":"OPENAIRE","@value":"doi_dedup___::6b9698303d8020e7b35377af34973e2f"},{"@type":"CROSSREF","@value":"10.2142/biophysics.10.1_references_DOI_N3jOqZSyv8ZqfVU73bdrlyi8FxS"},{"@type":"CROSSREF","@value":"10.2142/biophysico.12.0_37_references_DOI_N3jOqZSyv8ZqfVU73bdrlyi8FxS"},{"@type":"CROSSREF","@value":"10.1007/s12551-017-0337-8_references_DOI_N3jOqZSyv8ZqfVU73bdrlyi8FxS"},{"@type":"CROSSREF","@value":"10.2142/biophys.54.272_references_DOI_N3jOqZSyv8ZqfVU73bdrlyi8FxS"},{"@type":"CROSSREF","@value":"10.1364/oe.22.029611_references_DOI_N3jOqZSyv8ZqfVU73bdrlyi8FxS"},{"@type":"CROSSREF","@value":"10.3175/molsci.8.a0067_references_DOI_N3jOqZSyv8ZqfVU73bdrlyi8FxS"},{"@type":"CROSSREF","@value":"10.1016/j.bbabio.2014.07.020_references_DOI_N3jOqZSyv8ZqfVU73bdrlyi8FxS"}]}