{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390001205221689472.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2142/biophysics.11.67"}},{"identifier":{"@type":"URI","@value":"https://www.jstage.jst.go.jp/article/biophysics/11/0/11_67/_pdf"}},{"identifier":{"@type":"PMID","@value":"27493517"}},{"identifier":{"@type":"NAID","@value":"130004940815"}}],"resourceType":"学術雑誌論文(journal article)","dc:title":[{"@language":"en","@value":"<i>In vitro</i> directed evolution of alpha-hemolysin by liposome display"},{"@value":"In vitro directed evolution of alpha-hemolysin by liposome display"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"We have developed a method to enable <i>in vitro </i>directed evolution that can be applied to membrane proteins. This method, termed liposome display, uses liposomes as compartments in which membrane proteins are synthesized and as scaffolds for membrane protein integration. Thus, the synthesized membrane proteins are displayed on the surface of the liposome and exhibit their functions. A randomly mutated DNA library of the membrane protein was generated, encapsulated in the liposomes at the single-molecule level, and used to generate a liposome library. Liposomes displaying the desired membrane protein function were selected, thus accumulating the DNA molecule encoding the desired membrane protein. We have applied this method to alpha-hemolysin, a membrane protein derived from <i>Staphylococcus aureus</i>. Alpha-hemolysin forms a nanopore in the membrane, which allows the penetration of small molecules. We aimed to improve this nanopore activity by using the liposome display method. Consequently, alphahemolysin evolved and attained a higher specific affinity for the liposome membrane. In this review, we describe the essential characteristics of liposome display and the properties of the evolved alpha-hemolysin obtained by this method."}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1420845751151694720","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"50362653"},{"@type":"NRID","@value":"1000050362653"},{"@type":"NRID","@value":"9000006148139"},{"@type":"NRID","@value":"9000006132768"},{"@type":"NRID","@value":"9000002735627"},{"@type":"NRID","@value":"9000238282362"},{"@type":"NRID","@value":"9000356631356"},{"@type":"NRID","@value":"9000238279827"},{"@type":"NRID","@value":"9000259306392"},{"@type":"NRID","@value":"9000414354979"},{"@type":"NRID","@value":"9000018802283"},{"@type":"NRID","@value":"9000238862584"},{"@type":"NRID","@value":"9000288542259"},{"@type":"NRID","@value":"9000259304281"},{"@type":"NRID","@value":"9000303640400"},{"@type":"NRID","@value":"9000402800107"},{"@type":"NRID","@value":"9000006865558"},{"@type":"NRID","@value":"9000303639550"},{"@type":"NRID","@value":"9000259304284"},{"@type":"NRID","@value":"9000283593253"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/read0121158"}],"foaf:name":[{"@language":"en","@value":"Matsuura Tomoaki"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Japan Science and Technology (JST), ERATO, Yomo Dynamical Micro-scale Reaction Environment Project"},{"@language":"en","@value":"Graduate School of Engineering, Osaka University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001205221689473","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000283593254"}],"foaf:name":[{"@language":"en","@value":"Yomo Tetsuya"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Japan Science and Technology (JST), ERATO, Yomo Dynamical Micro-scale Reaction Environment Project"},{"@language":"en","@value":"Graduate School of Information Science and Technology, Osaka University"},{"@language":"en","@value":"Graduate School of Frontier Biosciences, Osaka University"},{"@language":"en","@value":"Earth-Life Science Institute, Tokyo Institute of Technology"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001205221689472","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000018278148"},{"@type":"NRID","@value":"9000239878919"},{"@type":"NRID","@value":"9000283593252"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/membrane"}],"foaf:name":[{"@language":"en","@value":"Fujii Satoshi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Japan Science and Technology (JST), ERATO, Yomo Dynamical Micro-scale Reaction Environment Project"}]}],"publication":{"publicationIdentifier":[{"@type":"EISSN","@value":"13492942"}],"prism:publicationName":[{"@language":"en","@value":"BIOPHYSICS"},{"@language":"en","@value":"BIOPHYSICS"}],"dc:publisher":[{"@language":"en","@value":"The Biophysical Society of Japan"},{"@language":"ja","@value":"日本生物物理学会"}],"prism:publicationDate":"2015","prism:volume":"11","prism:number":"0","prism:startingPage":"67","prism:endingPage":"72"},"reviewed":"false","url":[{"@id":"https://www.jstage.jst.go.jp/article/biophysics/11/0/11_67/_pdf"}],"availableAt":"2015","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=fluorescence-activated%20cell%20sorter","dc:title":"fluorescence-activated cell sorter"},{"@id":"https://cir.nii.ac.jp/all?q=membrane%20protein","dc:title":"membrane protein"},{"@id":"https://cir.nii.ac.jp/all?q=PURE%20system","dc:title":"PURE system"}],"project":[{"@id":"https://cir.nii.ac.jp/crid/1040282257217876864","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"25282239"},{"@type":"JGN","@value":"JP25282239"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25282239/"}],"notation":[{"@language":"ja","@value":"無細胞翻訳系を用いた膜タンパク質進化分子工学的手法の開発と応用"},{"@language":"en","@value":"Directed evolution of membrane proteins using cell-free protein synthesis system"}]},{"@id":"https://cir.nii.ac.jp/crid/1040282257254944768","@type":"Project","projectIdentifier":[{"@type":"KAKEN","@value":"26102528"},{"@type":"JGN","@value":"JP26102528"},{"@type":"URI","@value":"https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-26102528/"}],"notation":[{"@language":"ja","@value":"リポソーム内膜タンパク質合成系を用いた細胞膜動態の再構成"}]}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360002216881529088","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A controllable gene expression system in liposomes that includes a positive feedback loop"}]},{"@id":"https://cir.nii.ac.jp/crid/1360004233656754688","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Functional analysis of membranous Fo-<i>a</i> subunit of F1Fo-ATP synthase by <i>in vitro</i> protein 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