{"@context":{"@vocab":"https://cir.nii.ac.jp/schema/1.0/","rdfs":"http://www.w3.org/2000/01/rdf-schema#","dc":"http://purl.org/dc/elements/1.1/","dcterms":"http://purl.org/dc/terms/","foaf":"http://xmlns.com/foaf/0.1/","prism":"http://prismstandard.org/namespaces/basic/2.0/","cinii":"http://ci.nii.ac.jp/ns/1.0/","datacite":"https://schema.datacite.org/meta/kernel-4/","ndl":"http://ndl.go.jp/dcndl/terms/","jpcoar":"https://github.com/JPCOAR/schema/blob/master/2.0/"},"@id":"https://cir.nii.ac.jp/crid/1390001205227859968.json","@type":"Article","productIdentifier":[{"identifier":{"@type":"DOI","@value":"10.2198/jelectroph.49.5"}},{"identifier":{"@type":"NAID","@value":"10015570316"}},{"identifier":{"@type":"URI","@value":"https://search.jamas.or.jp/link/ui/2006205567"}}],"dc:title":[{"@language":"en","@value":"Two-dimensional electrophoretic profiling of normal human kidney: differential protein expression in glomerulus, cortex and medulla"}],"dc:language":"en","description":[{"type":"abstract","notation":[{"@language":"en","@value":"The diverse functions of the kidney are fundamentally fulfilled by the nephron, the functional unit of the kidney. The nephron consists of several functionally and structurally different segments, which spans the kidney from the cortex through the medulla. The functional specialization along the nephron and specific localization of the different segments should be reflected in the protein expression profiles of distinct anatomical compartments of the kidney. We performed a two-dimensional gel electrophoresis (2-DE)-based, quantitative, proteomic analysis to identify proteins differentially expressed in the cortex, medulla, and glomeruli purified from cortex of normal human kidney. The protein extracts of the three compartments obtained from 4 normal subjects were separated on 2-DE gels, and analyzed by using a 2-DE gel image analysis software. We detected 1,810 valid protein spots in the glomerulus, 1,758 in the cortex, and 1,275 spots in the medulla. Statistical analysis by one-way ANOVA followed by Tukey's post-hoc test revealed that 616 protein spots were differentially expressed in the glomeruli, 582 in the cortex, and 469 in the medulla. Among these protein spots, spots expressing in considerably higher relative amounts in one of the three compartments were selected and subjected to identification by peptide mass fingerprinting method with a MALDI-TOF mass spectrometer, which led to identification 19 protein spots preferentially expressed in the glomerulus, 35 in the cortex, and 21 in the medulla. The distinct protein expression profiles in different kidney compartments may serve as a resource for further study of physiology and pathophysiology of the human kidney.<br>"}],"abstractLicenseFlag":"disallow"}],"creator":[{"@id":"https://cir.nii.ac.jp/crid/1410001205227859969","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000002603093"}],"foaf:name":[{"@language":"en","@value":"Xu Bo"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Structural Pathology, Graduate School of Medical and Dental Sciences, Niigata University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001204568420481","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000006036809"},{"@type":"NRID","@value":"9000401732210"}],"foaf:name":[{"@language":"en","@value":"Yoshida Yutaka"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Structural Pathology, Graduate School of Medical and Dental Sciences, Niigata University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001205227859971","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000238412787"}],"foaf:name":[{"@language":"en","@value":"Zhang Ying"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Structural Pathology, Graduate School of Medical and Dental Sciences, Niigata University"}]},{"@id":"https://cir.nii.ac.jp/crid/1030003658314434817","@type":"Researcher","personIdentifier":[{"@type":"KAKEN_RESEARCHERS","@value":"00157950"},{"@type":"NRID","@value":"1000000157950"},{"@type":"NRID","@value":"9000391972411"},{"@type":"NRID","@value":"9000254451449"},{"@type":"NRID","@value":"9000261672847"},{"@type":"NRID","@value":"9000347092654"},{"@type":"NRID","@value":"9000399375056"},{"@type":"NRID","@value":"9000347092593"},{"@type":"NRID","@value":"9000006036810"},{"@type":"NRID","@value":"9000347091038"},{"@type":"NRID","@value":"9000347091919"},{"@type":"NRID","@value":"9000347092216"},{"@type":"NRID","@value":"9000021264528"},{"@type":"NRID","@value":"9000347091217"},{"@type":"NRID","@value":"9000391961008"},{"@type":"NRID","@value":"9000391970886"},{"@type":"NRID","@value":"9000347093554"},{"@type":"NRID","@value":"9000410910475"},{"@type":"NRID","@value":"9000391975916"},{"@type":"NRID","@value":"9000391975383"},{"@type":"NRID","@value":"9000347091318"},{"@type":"NRID","@value":"9000254060618"},{"@type":"NRID","@value":"9000254450076"},{"@type":"NRID","@value":"9000021457501"},{"@type":"NRID","@value":"9000391961674"},{"@type":"NRID","@value":"9000347092227"},{"@type":"NRID","@value":"9000399375525"},{"@type":"NRID","@value":"9000257888107"},{"@type":"NRID","@value":"9000391973280"},{"@type":"NRID","@value":"9000254450945"},{"@type":"NRID","@value":"9000391966651"},{"@type":"NRID","@value":"9000391969452"},{"@type":"NRID","@value":"9000257888022"},{"@type":"NRID","@value":"9000256085564"},{"@type":"NRID","@value":"9000347091242"},{"@type":"NRID","@value":"9000347092022"},{"@type":"NRID","@value":"9000254452154"},{"@type":"NRID","@value":"9000256085569"},{"@type":"NRID","@value":"9000399221131"},{"@type":"RESEARCHMAP","@value":"https://researchmap.jp/4zuhfuku-801ta"}],"foaf:name":[{"@language":"en","@value":"Yaoita Eishin"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Structural Pathology, Graduate School of Medical and Dental Sciences, Niigata University"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001205227859968","@type":"Researcher","personIdentifier":[{"@type":"NRID","@value":"9000001477200"}],"foaf:name":[{"@language":"en","@value":"Osawa Tetsuo"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Urology, Niigata City General Hospital"}]},{"@id":"https://cir.nii.ac.jp/crid/1410001205227859973","@type":"Researcher","foaf:name":[{"@language":"en","@value":"Yamamoto Tadashi"}],"jpcoar:affiliationName":[{"@language":"en","@value":"Department of Structural Pathology, Graduate School of Medical and Dental Sciences, Niigata University"}]}],"publication":{"publicationIdentifier":[{"@type":"PISSN","@value":"13499394"},{"@type":"EISSN","@value":"13499408"},{"@type":"CODEN","@value":"JEOLAS"},{"@type":"NCID","@value":"AA12015033"}],"prism:publicationName":[{"@language":"en","@value":"Journal of Electrophoresis"},{"@language":"en","@value":"J. Electrophor"}],"dc:publisher":[{"@language":"en","@value":"Japanese Electrophoresis Society"},{"@language":"ja","@value":"日本電気泳動学会"}],"prism:publicationDate":"2005","prism:volume":"49","prism:number":"1","prism:startingPage":"5","prism:endingPage":"13"},"reviewed":"false","dcterms:accessRights":"http://purl.org/coar/access_right/c_abf2","url":[{"@id":"https://search.jamas.or.jp/link/ui/2006205567"}],"availableAt":"2005","foaf:topic":[{"@id":"https://cir.nii.ac.jp/all?q=human%20kidney","dc:title":"human kidney"},{"@id":"https://cir.nii.ac.jp/all?q=glomerulus","dc:title":"glomerulus"},{"@id":"https://cir.nii.ac.jp/all?q=cortex","dc:title":"cortex"},{"@id":"https://cir.nii.ac.jp/all?q=medulla","dc:title":"medulla"},{"@id":"https://cir.nii.ac.jp/all?q=2-DE","dc:title":"2-DE"},{"@id":"https://cir.nii.ac.jp/all?q=differential%20expression","dc:title":"differential expression"}],"relatedProduct":[{"@id":"https://cir.nii.ac.jp/crid/1360004236789423616","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Comparison of human glomerulus proteomic profiles obtained from low quantities of samples by different mass spectrometry with the comprehensive database"}]},{"@id":"https://cir.nii.ac.jp/crid/1360848657249788544","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981468962900096","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"A thousand points of light: The application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics"}]},{"@id":"https://cir.nii.ac.jp/crid/1361981470892725632","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Differential expression of cytosolic proteins in the rat kidney cortex and medulla: Preliminary proteomics"}]},{"@id":"https://cir.nii.ac.jp/crid/1362262946073323392","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Differential expression of proteins in renal cortex and medulla: A proteomic approach11See Editorial by Bonventre, p. 1470."}]},{"@id":"https://cir.nii.ac.jp/crid/1362825896279707264","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Proteomics: the move to mixtures"}]},{"@id":"https://cir.nii.ac.jp/crid/1363670321184248960","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Gene Expression in the Normal Adult Human Kidney Assessed by Complementary DNA Microarray"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233268483181568","@type":"Article","relationType":["references"],"jpcoar:relatedTitle":[{"@value":"Two‐dimensional electrophoretic profiling of normal human kidney glomerulus proteome and construction of an extensible markup language (XML)‐based database"}]},{"@id":"https://cir.nii.ac.jp/crid/1364233270122785024","@type":"Article","relationType":["references","cites"],"jpcoar:relatedTitle":[{"@value":"Quantitation of microgram amounts of protein in two‐dimensional polyacrylamide gel electrophoresis sample buffer"}]},{"@id":"https://cir.nii.ac.jp/crid/1520572358344393728","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@value":"プロテオミクスの試料調製"},{"@language":"ja-Kana","@value":"プロテオミクス ノ シリョウ チョウセイ"}]},{"@id":"https://cir.nii.ac.jp/crid/1570009751802008192","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Differential expression of cytosolic proteins in the rat kidney cortex and medulla : proliminary proteomics"}]},{"@id":"https://cir.nii.ac.jp/crid/1570291225223679872","@type":"Article","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Proteomics : an infinite problem with infinite potential"}]},{"@id":"https://cir.nii.ac.jp/crid/1570572701188669056","@type":"Article","relationType":["isCitedBy"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Profiling of kidney vascular endothelial cell plasma membrane proteins by liquid chromatography-tandem mass spectrometry"}]},{"@id":"https://cir.nii.ac.jp/crid/1573950401485887872","@type":"Article","relationType":["cites"]},{"@id":"https://cir.nii.ac.jp/crid/1574231874897632512","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["cites"],"jpcoar:relatedTitle":[{"@language":"en","@value":"Two dimensional electrophoretic profiling of normal human kidney glomerulus proteome and construction of an extensible markup language (XML)-based database"}]},{"@id":"https://cir.nii.ac.jp/crid/2050588892097411200","@type":"Article","resourceType":"学術雑誌論文(journal article)","relationType":["isReferencedBy"],"jpcoar:relatedTitle":[{"@value":"Profiling of kidney vascular endothelial cell plasma membrane proteins by liquid chromatography-tandem mass spectrometry"}]}],"dataSourceIdentifier":[{"@type":"JALC","@value":"oai:japanlinkcenter.org:0024849753"},{"@type":"CROSSREF","@value":"10.2198/jelectroph.49.5"},{"@type":"CIA","@value":"10015570316"},{"@type":"OPENAIRE","@value":"doi_dedup___::c61643fa8b270023647dedd07a4e71ce"},{"@type":"CROSSREF","@value":"10.1016/j.jprot.2011.03.001_references_DOI_4h5z6J1PTk6JLOQNg08WkekqRBS"},{"@type":"CROSSREF","@value":"10.1007/s10157-012-0708-1_references_DOI_4h5z6J1PTk6JLOQNg08WkekqRBS"},{"@type":"CROSSREF","@value":"10.1186/1477-5956-9-47_references_DOI_4h5z6J1PTk6JLOQNg08WkekqRBS"}]}