Ectomycorrhizal formation on regenerated somatic pine plants after inoculation with Tricholoma matsutake

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  • 不定胚を経由したマツ苗へのマツタケ菌接種による外生菌根形成

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The formation of ectomycorrhizas of Tricholoma matsutake on somatic plants of three pine species (Pinus densiflora, Pin. thunbergii and Pin. taeda) was described. Mycorrhizas, which were similar to those previously reported on pine seedlings, formed on somatic pine plants in sterilized pumice supplemented with a nutrient solution containing glucose, dry yeast and FeCl_<3->. The shoot growth of the pine plants improved after mycorrhizal formation, whereupon the root to shoot ratio of the plant decreased. Mycorrhizal somatic pine plants can assist a molecular approach for investigating the metabolic mechanisms underlying symbiotic relationships between plants and mycorrhizal fungi.

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