Comparison of Serum Lipoprotein Quantitation between High Performance Liquid Chromatography and Agarose Gel Electrophoresis

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  • OKAZAKI Mitsuyo
    Laboratory of Chemistry, Department of General Education, Tokyo Medical and Dental University
  • TAKIZAWA Akira
    Department of Medical Technology, Tokyo Electronic College
  • HARA Ichiro
    Laboratory of Chemistry, Department of General Education, Tokyo Medical and Dental University

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Other Title
  • 血清リポタンパク質の定量における高速液体クロマトグラフィーとアガロースゲル電気泳動との比較
  • ケッセイ リポ タンパクシツ ノ テイリョウ ニ オケル コウソク エキタイ

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A new technique for serum lipoprotein quantitation by high performance liquid chromatography (HPLC) was evaluated by comparing the analytical data with the electrophoretic method using agarose gel film. The elution pattern of human serum (5μl) monitored by cholesterol gave three clear peaks according to particle-size differentiation: peak 1, chylomicrons+very low density lipoproteins (VLDL); peak 2, low density lipoproteins (LDL); peak 3, high density lipoproteins (HDL). As for a group (n=92) of normolipidemic men and women, cholesterol levels in these three fractions were determined by the HPLC method (X), and they were compared with those by the electrophoretic method (Y). A good correlation was obtained for peak 2 to β lipoprotein (r=0.961, Y=0.990X+3.43) and for peak 3 to α lipoprotein (r=0.911, Y=0.945X+7.21). Cholesterol level of peak 1 (chylomicrons +VLDL) was 5.3±4.0mg/dl, but that of pre-β liporrotein could not be measured by the electrophoretic method. Precision of determination (coefficient of variation, CV%) by the HPLC method was less than 1% both for peak 2 (LDL) and peak 3 (HDL). The HPLC method was found to have better precision and resolution for lipoprotein quantitation than the electrophoretic method.

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