Genotyping of the Q locus in wheat by a simple PCR-RFLP method
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- Asakura Nobuaki
- Laboratory of Biology, Faculty of Engineering, Kanagawa University
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- Mori Naoki
- Laboratory of Plant Genetics, Department of Biological and Environmental Science, Graduate School of Agricultural Science, Kobe University
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- Nakamura Chiharu
- Laboratory of Plant Genetics, Department of Biological and Environmental Science, Graduate School of Agricultural Science, Kobe University
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- Ohtsuka Ichiro
- Laboratory of Biology, Faculty of Engineering, Kanagawa University
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説明
The Q locus located on the long arm of chromosome 5A is a key factor in evolution and widespread cultivation of domesticated wheat. The Q locus pleiotropically affects many agronomically important traits including threshability, glume shape and tenacity, rachis fragility and others. Genotyping of the Q locus based on the complex traits is ambiguous due to their multi-genetic control through interactions with the Q locus. To determine the Q locus genotype of wheat accessions possessing A genome, we developed a method based on polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) analysis. The Q and q alleles were clearly distinguished by PCR-RFLP analysis at six conserved single nucleotide polymorphisms in common wheat and wild and cultivated einkorn, emmer and timopheevi wheat. The Q locus genotype of Triticum sinskajae, which is one of the einkorn wheat species and exhibits free-threshing trait, was determined to be qq as expected. This simple PCR-RLFP-based genotyping method should serve as a useful tool in studying the origin of Q and thus wheat evolution after domestication and the following widespread cultivation.<br>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 84 (3), 233-237, 2009
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390001205473392896
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- NII論文ID
- 10026091079
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- NII書誌ID
- AA11077421
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- COI
- 1:CAS:528:DC%2BD1MXhtlyqs7bP
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 10333076
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- PubMed
- 19745572
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可