Dissection of barley chromosomes 1H and 6H by the gametocidal system

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  • Ishihara Ayaka
    Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
  • Mizuno Nobuyuki
    Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
  • Islam Rafiqul A. K. M.
    School of Agriculture, Food and Wine, University of Adelaide
  • Doležel Jaroslav
    Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research
  • Endo Takashi R.
    Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research Palacký University Olomouc, Centre of the Region Haná for Biotechnological and Agricultural Research
  • Nasuda Shuhei
    Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University

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We dissected barley chromosomes 1H and 6H added to common wheat by the gametocidal system and identified structural changes of the chromosomes by fluorescence in situ hybridization and genomic in situ hybridization. We found five aberrations of chromosome 1H, all of which lacked the long arm: one small fragment with the subtelomeric HvT01 sequence, one terminal deletion, and three telocentric chromosomes of the short arm. We established 33 dissection lines carrying single aberrant 6H chromosomes, of which 15 were deletions, 16 were translocations and two were isochromosomes. We conducted PCR analysis of the aberrant barley chromosomes using 75 and 81 EST markers specific to chromosomes 1H and 6H, respectively. This enabled us to construct a cytological map of chromosome 6H and to compare it to the previously reported genetic map and also to the physical map, which were released by the International Barley Genome Sequencing Consortium. The marker orders on the three maps were largely in agreement. The cytological map had better resolution in the proximal region of chromosome 6H than the corresponding genetic map. We discuss some of the discrepancies in marker order between the three maps that might be due to intraspecific polymorphism and gene duplication, as well as to technical problems inherent in the physical mapping process.

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